A5034496794- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Hypothalamic control of reproductive hormones
- Neuroendocrine regulation and behavior
- Neurotransmitter Receptor Influence on Behavior
- Olfactory and Sensory Function Studies
- Attention Deficit Hyperactivity Disorder
- Effects and risks of endocrine disrupting chemicals
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Receptors and Signaling
- Advanced Proteomics Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Pain Mechanisms and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Plant Genetic and Mutation Studies
- Biochemical Analysis and Sensing Techniques
- Medicinal Plants and Neuroprotection
- Genomics, phytochemicals, and oxidative stress
- Diet and metabolism studies
- RNA Research and Splicing
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Plant Stress Responses and Tolerance
Toho University
2012-2023
National Institute for Environmental Studies
2008-2014
National Institute of Advanced Industrial Science and Technology
2002-2012
The University of Tokyo
2010
Seoul National University
2008
Hokkaido University
2008
Fukuoka Dental College
2008
Tokyo Women's Medical University
2007
New Energy and Industrial Technology Development Organization
2002-2004
University of Utah
1998
MALAT‐1, a long non‐coding RNA, is associated with metastasis, but its role in the metastatic process remains unknown. Here, we show that short‐interfering RNA‐mediated MALAT‐1 silencing impaired vitro cell motility of lung cancer cells and influenced expression numerous genes. In these genes, knockdown any one CTHRC1 , CCT4 HMMR or ROD1 clearly inhibited migration. cells, pre‐mRNA levels were decreased some not all Thus, our findings suggest novel class RNA promotes through transcriptional...
MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote localization and also provide evidence involved in regulation gene expression. Heterokaryon assays revealed does not shuttle between nucleus cytoplasm. RNAi-mediated repression speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted diffusion nucleoplasm. We demonstrated contains two...
Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis gene(s) has revolutionized the 21st century biological science due its tremendous application quantitative genotyping, genetic variation inter intra organisms, early diagnosis disease, forensic, name a few. We comprehensively review various aspects PCR, including technological refinement all scientific fields ranging from medical...
Ozone (O3), a serious air pollutant, is known to significantly reduce photosynthesis, growth, and yield cause foliar injury senescence. Here, integrated transcriptomics, proteomics, metabolomics approaches were applied investigate the molecular responses of O3 in leaves 2-week-old rice (cv. Nipponbare) seedlings exposed 0.2 ppm for period 24 h. On basis morphological alteration O3-exposed leaves, transcript profiling genes was performed 1, 12, h using DNA microarray chip. A total 1535...
Abstract We examined the effects of bisphenol A, an endocrine disruptor, on rat behavioral and cellular responses. Single intracisternal administration A (0.2‐20 μg) into 5‐day‐old male Wistar rats caused significant hyperactivity at 4–5 weeks age. Rats were about 1.6‐fold more active in nocturnal phase after both 2 20 μg than control rats. The response was dose‐dependent. Based DNA macroarray analyses midbrain, decreased by twofold gene expression levels dopamine D4 receptor 4 age...
Royal jelly (RJ) is an exclusive food for queen honey bee (Apis mellifera L.) that synthesized and secreted by young worker bees. RJ also widely used in medical products, cosmetics, as health foods. However, little known about functionality the total protein components, although recent research attempting to unravel proteome. We have embarked on a detailed investigation of proteome, using modified extraction protocol two complementary proteomics approaches, one- two-dimensional gel...
Helicobacter pylori infection, which causes peptic ulcers and gastric cancer, is considered a possible cause of halitosis. Recently, the oral cavity was identified as H. reservoir, particularly in presence periodontal disease, The purpose this study to evaluate by PCR prevalence saliva subjects complaining Samples were obtained from 326 non-dyspeptic subjects, comprising 251 with actual malodour 75 without DNA extracted samples, periodontopathic bacteria including Porphyromonas gingivalis,...
To investigate the mechanisms underlying motor hyperactivity, we performed intracisternal injection of 6-hydroxydopamine or endocrine disruptors in rats on postnatal day 5. 6-Hydroxydopamine (100 microg, 488 nmol) caused a significant increase spontaneous activities at 4 weeks age. Gene-expression profiling using cDNA membrane array revealed alterations several classes gene 8 In midbrain, expression was enhanced dopamine transporter 1; platelet-derived growth factor receptor; receptor D4;...
Abstract Endocrine disruptors possibly exert effects on neuronal functions leading, in particular, to behavioural alterations. In this study, we examined the of dicyclohexylphthalate (DCHP), an endocrine disruptor, rat and cellular responses. Single intracisternal administration DCHP (0.87–87 nmol) into 5‐day‐old male Wistar rats caused significant hyperactivity at 4–5 weeks age. It was about 1.4‐fold more active nocturnal phase after 87 nmol than control ( p < 0.001). The response had a...
Essential oils are mainly administered by inhalation. Administration inhalation is considered to occur through two pathways, neurological transfer and pharmacological transfer. However, the relationship between routes not clear. To clarify this relationship, we α-pinene, which has an anxiolytic-like effect, mice. Emotional behavior accumulation expression of relevant mRNAs in brain (brain-derived neurotrophic factor (BDNF); tyrosine hydroxylase (TH)) were examined following inhaled...