A5069633631- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Antimicrobial Peptides and Activities
- Ion-surface interactions and analysis
- Photoreceptor and optogenetics research
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Advanced Glycation End Products research
- Retinal Development and Disorders
- Advanced Chemical Sensor Technologies
- Enzyme Structure and Function
- Cholinesterase and Neurodegenerative Diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Viral Infectious Diseases and Gene Expression in Insects
- Biopolymer Synthesis and Applications
- Microbial metabolism and enzyme function
- Biochemical and Structural Characterization
- Antioxidant Activity and Oxidative Stress
- Diabetes and associated disorders
- Photochromic and Fluorescence Chemistry
- Retinoids in leukemia and cellular processes
National Institute of Advanced Industrial Science and Technology
2015-2025
KM Biologics (Japan)
2022
Japan Advanced Institute of Science and Technology
2018
Yokohama City University
2008
National Institute of Infectious Diseases
1999-2005
University of Electro-Communications
1993-2000
Nagoya University
1999
Shizuoka University
1999
University of Oklahoma Health Sciences Center
1994-1997
The turning point between apoptosis and necrosis induced by hydrogen peroxide (H2O2) have been investigated using human T-lymphoma Jurkat cells. Cells treated with 50 microM H2O2 exhibited caspase-9 caspase-3 activation, finally leading to apoptotic cell death. Treatment 500 did not exhibit caspase activation changed the mode of death necrosis. On other hand, release cytochrome c from mitochondria was observed under both conditions. H2O2, but caused a marked decrease in intracellular ATP...
Fine metal or oxide powder as an alternative to conventional organic matrices in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been utilized successfully for lower molecular analytes, poly(ethylene glycol) 200 (PEG 200) and methyl stearate. Eleven kinds of particle, Al, Mn, Mo, Si, Sn, SnO2, TiO2, W, WO3, Zn ZnO, were evaluated. The analyte was mixed with a (inorganic matrix) particle diameter tens micrometers liquid dispersant, followed by...
Fine metal or oxide powder as an alternative to conventional organic matrices in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been utilized successfully for lower molecular analytes, poly(ethylene glycol) 200 (PEG 200) and methyl stearate. Eleven kinds of particle, Al, Mn, Mo, Si, Sn, SnO2, TiO2, W, WO3, Zn ZnO, were evaluated. The analyte was mixed with a (inorganic matrix) particle diameter tens micrometers liquid dispersant, followed by...
A multivalent polymer of vancomycin, synthesized via ring-opening metathesis polymerization (ROMP), exhibited significant enhancement antibacterial activity against vancomycin-resistant enterococi (VRE).
Main text Inherited metabolic disorders affect approximately 1 in 1000 babies; amino acid are less frequent, at 6000. Babies diagnosed with require constant monitoring of blood concentration, especially for disease states such as phenylketonuria. In addition, measurement may aid the evaluation several other neurological and nutritional disorders. Free acids healthy patients typically low mg/kg range but, example, elevated phenylalanine indicates phenylketonuria state. Evidence successful...
We have developed a novel amino acid analysis method using derivatization of multiple functional groups (amino, carboxyl, and phenolic hydroxyl groups).
Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received the skin to spinal cord. Here we undertook a proteomic approach uncover polarity of primary fibers. Lumbar nerve segments, peripheral central DRG, were dissected 5-wk-old Wistar rats lysates subjected large-sized 2-DE at pH 5-6. Among approximately 800 protein spots detected fractions, one unique fraction with MW 60 kDa pI 5.6 was identified as an...
CASMI (Critical Assessment of Small Molecule Identification) is a contest in which participants identify the molecular formula and chemical structure challenging molecules using blind mass spectra as challenge data. Seven research teams participated CASMI2013. The winner CASMI2013 was team Andrew Newsome Dejan Nikolic, University Illinois at Chicago, IL, USA. identified 15 among 16 by manually interpreting data searching in-house public spectral databases, substance literature databases....
The oxidative modification of low-density lipoprotein (LDL) and subsequent alteration endothelial cell function are generally accepted as an important early event in the pathogenesis atherosclerosis. To understand mechanism by which oxidized LDL (oxLDL) causes dysfunction cells, human umbilical vein cells (HUVEC) were exposed to oxLDL at a concentration that induces cellular dysfunction, proteomic analysis was carried out, together with lipid peroxidation products. Time-dependent...
The reagents 19 hydrazide and 14 hydrazine were examined to function as reactive matrices for matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) detect gaseous aldehydes. Among them, two (2‐hydroxybenzohydrazide 3‐hydroxy‐2‐naphthoic acid hydrazide) [2‐hydrazinoquinoline 2,4‐dinitrophenylhydrazine (DNPH)] found react efficiently with carbonyl groups of aldehydes (formaldehyde, acetaldehyde propionaldehyde); these are the main factors sick building syndrome operate...
To examine the role of thioredoxin-1 (TRX-1), an endogenous protein with a variety redox-related roles, in formation choroidal neovascularization (CNV).CNV was induced by laser photocoagulation ocular fundus wild-type and transgenic mice overexpressing human TRX-1 (TRX-1 Tg). Mice were injected intraperitoneally TRX-1, mutant TRX, or vehicle. The incidence CNV evaluated lectin staining. Leukocyte recruitment C3b deposition after injury determined immunohistochemistry Western blotting....