Huntington's disease (HD) is a fatal neurodegenerative disorder. Despite tremendous effort to develop therapeutic tools in several HD models, there no effective cure at present. Acidosis has been observed previously cellular and vivo models as well the brains of patients. Here we challenged with amiloride (Ami) derivative benzamil (Ben), chemical agent used rescue acid-sensing ion channel (ASIC)-dependent acidotoxicity, examine whether chronic acidosis an important part pathomechanism these...

Osteoclasts and osteoblasts are responsible for strict bone maintenance with a balance between formation resorption by interacting each other. Recently, it has been revealed that osteoblasts/stromal cells regulate differentiation of osteoclasts/hematopoietic two factors, the receptor activator nuclear factor-kappaB (NF-kappaB) ligand (RANKL) on plasma membrane, secreted osteoprotegerin (OPG). However, no factors have yet reported which cells. To elucidate possibility signal transduction from...

Diagnostic biomarkers of major depressive disorder, bipolar and schizophrenia are urgently needed, because none currently available.We performed a comprehensive metabolome analysis plasma samples from drug-free patients with disorder (n=9), (n=6), (n=17), matched healthy controls (n=19) (cohort 1) using liquid chromatography time-of-flight mass spectrometry. A significant effect diagnosis was found for 2 metabolites: nervonic acid cortisone, being the most significantly altered. The...

Pyrroloquinoline quinone (PQQ) is believed to be an important factor for mammalian growth and development has, therefore, been declared a vitamin by some researchers. However, this issue remains controversial, from nutritional viewpoint, accurate determination of PQQ levels in variety foods very important. Here, we describe simple, highly sensitive, selective method quantitative analysis PQQ. Liquid or aqueous extracts solid were analyzed using high-performance liquid chromatography (HPLC)...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKeramamides B .apprx. D, novel peptides from the Okinawan marine sponge Theonella spJunichi Kobayashi, Fumio Itagaki, Hideyuki Shigemori, Masami Ishibashi, Kazuhiko Takahashi, Michiko Ogura, Shigeharu Nagasawa, Takemichi Nakamura, Hiroshi Hirota, and Cite this: J. Am. Chem. Soc. 1991, 113, 20, 7812–7813Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September...

A novel peptide, keramamide 1, has been isolated from the Okinawan marine sponge Theonella sp. and structure established as a unique hexapeptide containing hitherto unknown amino acid 6-chloro-5-hydroxy-N-methyltryptophan, possessing an unusual ureido bond. The structural assignment was made on basis of spectroscopic results (two-dimensional NMR: 1H–1H COSY, NOESY, ROESY, COLOC, HMQC, HMBC HOHAHA; FAB MS/MS).

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKeramamide F, a new thiazole-containing peptide from the Okinawan marine sponge Theonella spFumio Itagaki, Hideyuki Shigemori, Masami Ishibashi, Takemichi Nakamura, Takuma Sasaki, and Junichi KobayashiCite this: J. Org. Chem. 1992, 57, 20, 5540–5542Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September...

We propose a novel method for the identification and C-terminal characterization of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Proteins were digested in buffer solution containing 50% 18O-labeled water, mixtures 18O/16O-labeled peptides analyzed nanoelectrospray Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). This was evaluated using horse skeletal muscle myoglobin as model protein SDS gel. The high resolution FT-ICR MS...

A mollusk-specific toxin, TxVIIA, having potent paralytic activity was isolated from the venom of sea snail Conus textile (Fainzilber M et al., 1991, Eur J Biochem 202:589-595). The structure reported above based upon amino acid analysis and Edman degradation. We have recently reinvestigated this toxin employing some most novel techniques in mass spectrometry. now report a revised primarily on high-energy collision-induced dissociation two Asp17-N peptides reduced, pyridinylethyl derivative...

We previously found that phosphatidylglucoside (PtdGlc), a novel glycolipid expressed in HL60 cells, plays role forming signaling microdomains involved cellular differentiation. Because cells contain minute levels of PtdGlc, pure PtdGlc is very difficult to isolate. Thus, its complete structure has never been assessed. To aid analyzing we generated PtdGlc-specific monoclonal antibody, DIM21, by immunizing mice with detergent-insoluble membranes isolated from [Yamazaki, Y., et al. (2006) J....

The structures of intact choline phospholipids were determined by positive and negative ion mode fast atom bombardment mass spectrometry, tandem B2/E B/E constant linked scan spectrometry. molecular weight the lipid could be clearly appearance [M + H]+ or Na]+ in triplet ions, e.g., - 15]-, 60]-, 86]-, mode. ions assigned to CH3]-, HN(CH3)3]-, CH2 = CHN(CH3)3]-, respectively, MS/MS each ion, origin was found as matrix-ion adduct target molecule using technique. polar group identified...

CASMI (Critical Assessment of Small Molecule Identification) is a contest in which participants identify the molecular formula and chemical structure challenging molecules using blind mass spectra as challenge data. Seven research teams participated CASMI2013. The winner CASMI2013 was team Andrew Newsome Dejan Nikolic, University Illinois at Chicago, IL, USA. identified 15 among 16 by manually interpreting data searching in-house public spectral databases, substance literature databases....

A novel γ-carboxyglutamate-containing peptide, designated γ-conotoxin-PnVIIA, is described from the venom of molluscivorous snail Conus pennaceus. γPnVIIA triggers depolarization and firing action potential bursts in caudodorsal neurons Lymnaea. This effect due to activation or enhancement a slow inward cation current that may underly endogenous bursting activity these neurons. The amino acid sequence was determined as DCTSWFGRCTVNSγCCSNSCDQTYCγLYAFOS (where γ γ-carboxyglutamate, O...

Abstract Telomestatin, a strong telomerase inhibitor with G-quadruplex stabilizing activity, is potential therapeutic agent for treating cancers. Difficulties in isolating telomestatin from microbial cultures and chemical synthesis are bottlenecks impeding the wider use. Therefore, improvement production structural diversification required further utilization application. Here, we discovered gene cluster responsible biosynthesis, achieved of by heterologous expression this engineered...

A new cinnamic acid derivative, (E)-3-[4-hydroxy-3-((E)-3-formyl-2-butenyl)phenyl]-2- propenoic (20) has been isolated from the ethanol extract of Brazilian green propolis along with three known derivatives, 3,4-dihydroxy-5-prenyl-(E)-cinnamic (4), capillartemisin (6), and 2,2-dimethylchromene-6-(E)-propenoic (8), a flavonoid, dihydrokaempferide (16) by liquid–liquid participation, series column chromatography preparative HPLC. Their structures have determined spectroscopic analyses chemical...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTheonezolide a: a novel polyketide natural product from the Okinawan marine sponge Theonella spJunichi Kobayashi, Kazuhiko Kondo, Masami Ishibashi, Markus R. Walchli, and Takemichi NakamuraCite this: J. Am. Chem. Soc. 1993, 115, 15, 6661–6665Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/ja00068a024https://doi.org/10.1021/ja00068a024research-articleACS...