A5051175802- Microbial Natural Products and Biosynthesis
- Gastroesophageal reflux and treatments
- Esophageal and GI Pathology
- Gastric Cancer Management and Outcomes
- Genomics and Phylogenetic Studies
- Eosinophilic Esophagitis
- Dysphagia Assessment and Management
- Metastasis and carcinoma case studies
- Plant biochemistry and biosynthesis
- Gastrointestinal disorders and treatments
- Gastrointestinal Tumor Research and Treatment
- Esophageal Cancer Research and Treatment
- Plant Disease Resistance and Genetics
- Synthetic Organic Chemistry Methods
- Helicobacter pylori-related gastroenterology studies
- Magnetic Bearings and Levitation Dynamics
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Railway Systems and Energy Efficiency
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Steroid Chemistry and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Electric Motor Design and Analysis
Kitasato University
2014-2024
Keio University
2024
Keio University Shonan Fujisawa
2024
Showa University Koto Toyosu Hospital
2015-2023
Showa University
2014-2023
The Japanese Society of Gastroenterological Surgery
2020-2023
Hiroshima University Hospital
2023
Hudson Institute
2023
Iwate Medical University
2020-2023
Koto Hospital
2014-2021
Species of the genus Streptomyces are major pharmaceutical interest because they synthesize a variety bioactive secondary metabolites. We have determined complete nucleotide sequence linear chromosome avermitilis. S. avermitilis produces avermectins, group antiparasitic agents used in human and veterinary medicine. The genome contains 9,025,608 bases (average GC content, 70.7%) encodes at least 7,574 potential open reading frames (ORFs). Thirty-five percent ORFs (2,664) constitute 721...
Streptomyces avermitilis is a soil bacterium that carries out not only complex morphological differentiation but also the production of secondary metabolites, one which, avermectin, commercially important in human and veterinary medicine. The major interest this genus diversity its metabolites as an industrial microorganism. A factor prominence producer variety possession several metabolic pathways for biosynthesis. Here we report sequence analysis S. avermitilis, covering 99% genome. At...
ABSTRACT We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists 8,545,929 base pairs (bp), with average G+C content 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) 132,910 bp each....
Significance Terpenes are generally considered to be plant or fungal metabolites, although a small number of odoriferous terpenes bacterial origin have been known for many years. Recently, extensive genome sequencing and bioinformatic analysis deduced proteins using profile based on hidden Markov model revealed 262 distinct predicted terpene synthases. Although these presumptive synthase genes seem silent in their parent microorganisms, controlled expression an engineered heterologous...
To construct a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis, the genome industrial microorganism Streptomyces avermitilis was systematically deleted to remove nonessential genes. A region more than 1.4 Mb stepwise from 9.02-Mb S. linear chromosome generate series defined deletion mutants, corresponding 83.12–81.46% wild-type chromosome, that did not produce any major endogenous metabolites found in parent strain. The suitability mutants...
<b>Background and study aims:</b> Resection of submucosal tumors by means endoscopy has been reported using a variety techniques, but cannot be performed safely in originating from the muscularis propria. Using tunnel created technique peroral endoscopic myotomy (POEM), we report first series describing new tumor resection (SET) for esophagus cardia. <b>Patients methods:</b> SET was attempted nine consecutive patients with (size > 2 cm) either or cardia clinical indications lesion removal....
An industrial microorganism, Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as versatile model host for heterologous expression genes encoding secondary metabolite biosynthesis. Twenty the entire biosynthetic gene clusters metabolites were successively cloned and introduced into S. avermitilis SUKA17 or 22. Almost all transformants carrying cluster produced result introduced. A few unable to produce metabolites, but their...
Analysis of the gene cluster from Streptomyces avermitilis that governs biosynthesis polyketide anthelmintic avermectin revealed it contains four large ORFs encoding giant multifunctional polypeptides synthase (AVES 1, AVES 2, 3, and 4). These clustered genes responsible for together encode 12 homologous sets enzyme activities (modules), each catalyzing a specific round chain elongation. The are organized as two six modular repeats, aveA1-aveA2 aveA3-aveA4 , which convergently transcribed....
To identify the genes for biosynthesis of off-flavor terpenoid alcohol, 2-methylisoborneol (2-MIB), key encoding monoterpene cyclase were located in bacterial genome databases by using a combination hidden Markov models, protein-family search, and sequence alignment their gene products. Predicted terpene cyclases classified into three groups: sesquiterpene, diterpene, other cyclases. Genes group that form an operon with S-adenosyl-l-methionine (SAM)-dependent methyltransferase found data...
Gram-positive bacteria of the genus Streptomyces are industrially important microorganisms, producing >70% commercially antibiotics. The production these compounds is often regulated by low-molecular-weight bacterial hormones called autoregulators. Although 60% strains may use γ-butyrolactone–type molecules as autoregulators and some furan-type molecules, little known about signaling used to regulate antibiotic in many other members this genus. Here, we purified a molecule (avenolide)...
Abstract Background Peroral endoscopic myotomy (POEM) has become the minimally invasive treatment for achalasia; however, gastroesophageal reflux (GER) post-POEM been reported. A pilot study was conducted in which an fundoplication added to standard POEM (POEM + F) procedure overcome this issue. We report technical details of F and short-term safety results. Methods performed 21 patients. After completing myotomy, endoscope advanced from submucosal tunnel into peritoneal cavity. partial...
Abstract Polyketides form many clinically valuable compounds. However, manipulation of their biosynthesis remains highly challenging. An understanding gene cluster evolution provides a rationale for reprogramming the biosynthetic machinery. Herein, we report characterization giant modular polyketide synthases (PKSs) responsible production aminopolyol polyketides. Heterologous expression over 150 kbp clusters successfully afforded products, whose stereochemistry was established by taking...
ABSTRACT Mycosporines and mycosporine-like amino acids (MAAs), including shinorine (mycosporine-glycine-serine) porphyra-334 (mycosporine-glycine-threonine), are UV-absorbing compounds produced by cyanobacteria, fungi, marine micro- macroalgae. These MAAs have the ability to protect these organisms from damage environmental UV radiation. Although no reports described production of corresponding genes involved in MAA biosynthesis Gram-positive bacteria date, genome mining bacterial database...
Versipelostatin (VST) is an unusual 17-membered macrocyclic polyketide product that contains a spirotetronate skeleton. In this study, the entire VST biosynthetic gene cluster (vst) spanning 108 kb from Streptomyces versipellis 4083-SVS6 was identified by heterologous expression using bacterial artificial chromosome vector. Here, we demonstrate enzyme, VstJ, catalyzes stereoselective [4+2]-cycloaddition between conjugated diene and exocyclic olefin of newly tetronate-containing intermediate...
Abstract Background The incidence of proton pump inhibitor (PPI)-refractory gastroesophageal reflux disease (GERD) has been increasing. While surgical intervention with Laparoscopic Nissen Fundoplication remains the gold standard, less invasive anti-reflux interventions are desired. We have developed a minimally mucosal ablation (ARMA) treatment. Herein, we report its technical details and describe feasibility, safety, efficacy in PPI-refractory GERD. Methods conducted prospective...