A5001137629- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Reproductive biology and impacts on aquatic species
- Insect and Arachnid Ecology and Behavior
- Advanced Chemical Sensor Technologies
- Aquaculture Nutrition and Growth
- Insect and Pesticide Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hemoglobinopathies and Related Disorders
- Marine Toxins and Detection Methods
- Microbial Community Ecology and Physiology
- Tardigrade Biology and Ecology
- Invertebrate Taxonomy and Ecology
- Marine and coastal ecosystems
- Sperm and Testicular Function
- Marine Biology and Ecology Research
- Erythrocyte Function and Pathophysiology
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Neonatal Health and Biochemistry
- Coral and Marine Ecosystems Studies
- Isotope Analysis in Ecology
- Venomous Animal Envenomation and Studies
Chubu University
2016-2025
Imamura Hospital
2025
University of Missouri
2024
Kasugai Municipal Hospital
2023
Aichi University
2023
Nagoya University
2009-2020
Thrombosis Research Institute
2018
University College London
2018
National Institute for Basic Biology
1997-2012
ARC Centre of Excellence for Coral Reef Studies
2007
Bioluminescence is found across the entire tree of life, conferring a spectacular set visually oriented functions from attracting mates to scaring off predators. Half dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which only bacteria. Here, we report identification fungal luciferase and three other key enzymes together form biosynthetic cycle caffeic...
Fireflies and their luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin bioluminescence within beetles remains unclear. To shed light on this long-standing question, we sequenced genomes two species that diverged over 100 million-years-ago: North American Photinus pyralis Japanese Aquatica lateralis. compare bioluminescent origins, also genome a related click beetle, Caribbean Ignelater...
Abstract Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in chemical reactions that light have not been reported. We identified compound luciferin 3‐hydroxyhispidin, which is biosynthesized by oxidation precursor hispidin, and plant secondary metabolite. The does share structural similarity with other eight luciferins. Furthermore, it was shown 3‐hydroxyhispidin leads to bioluminescence extracts from four diverse...
Study of fungal bioluminescence mechanisms generates development a multicolor enzymatic chemiluminescence system.
Involvement of Ad4BP/SF-1 in the ovarian cytochrome P-450 aromatase (oP450arom) gene expression was investigated using follicles Nile tilapia, possessing an average 14-day spawning cycle. The promoter region (5' flanking region) oP450arom cloned from tilapia contains two Ad4 binding sites. Subsequently, a cDNA encoding follicles. It is expressed gonadal tissues, brain, and kidney. Oligonucleotide probes containing putative orphan nuclear receptor motifs (derived gene) formed complexes with...
Firefly luciferase can catalyze the formation of fatty acyl‐CoA via acyl‐adenylate from acid in presence ATP, Mg 2+ and coenzyme A (CoA). long chain (C 16 –C 20 ), produced by a North American firefly ( Photinus pyralis ) Japanese Luciola cruciata was isolated identified matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry analysis. Of number substrates tested, linolenic 18:3 arachidonic 20:4 appear to be suitable for synthesis. This evidence suggests that within...
Renovascular hypertension (RVHT) is most commonly caused by renal artery stenosis (RAS) secondary to arteriosclerosis. Other causes of RVHT include fibromuscular dysplasia (FMD) and other rare causes, such as Takayasu arteritis (TA). A male patient in his early 20s presented with hypertension. Laboratory findings were positive for hypokalemia well elevations plasma renin activity aldosterone concentration. Plain computed tomography revealed atrophy the right kidney, magnetic resonance...
Venomous mammals are rare, and their venoms have not been characterized. We purified characterized the blarina toxin (BLTX), a lethal mammalian venom with tissue kallikrein-like activity from submaxillary sublingual glands of short-tailed shrew Blarina brevicauda . Mice administered BLTX i.p. developed irregular respiration, paralysis, convulsions before dying. Based on amino acid sequence protein, we cloned cDNA. It consists prosequence an active form 253 aa typical catalytic triad serine...
Click beetles (Coleoptera: Elateridae) represent one of the largest groups beetle insects. Some click in larval form, known as wireworms, are destructive agricultural pests. Morphological identification is generally difficult and requires taxonomic expertise. This study reports on DNA barcoding Japanese to enable their rapid accurate identification. We collected assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% common species found...
Marine polychaetes Odontosyllis undecimdonta , commonly known as fireworms, emit bright blue-green bioluminescence. Until the recent identification of luciferase enzyme, little progress had been made toward characterizing key components this bioluminescence system. Here we present biomolecular mechanisms enzymatic (leading to light emission) and nonenzymatic (dark) oxidation pathways newly described O. luciferin. Spectral studies, including 1D 2D NMR spectroscopy, mass spectrometry, X-ray...
Resurrection of extinct firefly luciferases sheds light on the evolutionary history ancient glow beetles.
Meiotic maturation in fish is accomplished by maturation-inducing hormones. 17α,20β-Dihydroxy-4-pregnen-3-one (17α,20β-DP) was identified as the hormone of several teleosts, including Nile tilapia. A cDNA encoding 20β-hydroxysteroid dehydrogenase (20β-HSD), enzyme that converts 17α-hydroxyprogesterone to 17α,20β-DP, cloned from ovarian follicle Genomic Southern analysis indicated 20β-HSD probably exists a single copy genome. The Escherichia coli-expressed product oxidized both carbonyl and...
Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property protein structure of firefly luciferase have been investigated, its cDNA has used for various assay systems. chemical luciferin was identified as the D-form 1963 studies on biosynthesis began early 1970's. Incorporation experiments using (14)C-labeled compounds were performed, cysteine benzoquinone/hydroquinone proposed to be biosynthetic component luciferin. However,...
Bioluminescent enzyme of the golden sweeper is supplied from luminous crustacean diet.