A5006502944- bioluminescence and chemiluminescence research
- Spaceflight effects on biology
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Laser Applications in Dentistry and Medicine
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Light effects on plants
- Plant and Biological Electrophysiology Studies
- Nonmelanoma Skin Cancer Studies
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Cardiac electrophysiology and arrhythmias
- Transgenic Plants and Applications
- Medical and Biological Ozone Research
- Plant Pathogens and Fungal Diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Microbial Natural Products and Biosynthesis
- Photodynamic Therapy Research Studies
- Plant-Microbe Interactions and Immunity
- Bacillus and Francisella bacterial research
- Biofield Effects and Biophysics
Institute of Bioorganic Chemistry
2021-2024
Planta
2019-2024
Institute of Theoretical and Experimental Biophysics
2008-2021
Russian Academy of Sciences
2008-2010
Bioluminescence is found across the entire tree of life, conferring a spectacular set visually oriented functions from attracting mates to scaring off predators. Half dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which only bacteria. Here, we report identification fungal luciferase and three other key enzymes together form biosynthetic cycle caffeic...
Abstract The discovery of the bioluminescence pathway in fungus Neonothopanus nambi enabled engineering eukaryotes with self-sustained luminescence. However, brightness luminescence heterologous hosts was limited by performance native fungal enzymes. Here we report optimized versions that enhance one to two orders magnitude plant, and mammalian hosts, enable longitudinal video-rate imaging.
Jasmonic and salicylic acids are the major hormones involved in plant response to pests pathogens. Here, we engineered autoluminescent plants that report activity of these with up 53-fold contrast. Using consumer-grade cameras, imaged reporter Arabidopsis thaliana Nicotiana benthamiana throughout normal development, attacks
The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. starts from hispidin biosynthesis—a step catalyzed by a large polyketide synthase that requires posttranslational modification for activity. Here, we report identification of alternative compact synthases encoded phylogenetically diverse group plants. A hybrid combines plant and genes is more compact, not dependent on availability machinery...
Abstract In contrast to fluorescent proteins, light emission from luciferase reporters requires exogenous addition of a luciferin substrate. Bacterial bioluminescence has been the single exception, where an operon five genes is sufficient produce autonomously. Although commonly used in prokaryotic hosts, toxicity aldehyde substrate limited its use eukaryotes 1 . Here we demonstrate autonomous luminescence multicellular eukaryotic organism by incorporating recently discovered fungal...
Noncoding RNAs play a crucial role in various cellular processes living organisms, and RNA functions heavily depend on molecule structures composed of stems, loops, tertiary motifs. Among those, the most frequent are A-minor interactions, which often involved formation more complex motifs such as kink-turns pseudoknots. We present novel classification A-minors terms secondary structure where each nucleotide an is attributed to stem or loop, pair nucleotides their relative position within...
We report a systematic comparison of 19 plant promoters and 20 promoter-terminator combinations in two expression systems: agroinfiltration Nicotiana benthamiana leaves, tabacum BY-2 cell packs. The set tested comprised those not present previously published work, including several computationally predicted synthetic validated here for the first time. EGFP driven by different varied more than orders magnitude was largely consistent between systems. confirmed previous reports significant...
Across-bulged мотив -новый третичный РНК, содержащий А-минор взаимодействияБаулин Е.Ф. 1, 2 , Фахранурова Л
The effect of artificial sunlight (AS) from a xenon source and converted AS with an additional orange-red luminescent (λ<sub>MAX</sub>=626 nm) component (AS+L) on the development mouse zygotes was investigated. A plastic screen photoluminophore layer used for production this (L) component. single short-term (15 min) exposure produced long-term stable positive early embryo mice, which persisted during several days. After to AS+L, stimulating influence preimplantation observed, in...
We propose a "useful sun" strategy with application of photoluminophore that absorbs part the UV component sunlight and converts it into visible light. As result, "harmful" sun radiation becomes useful. The present study was designed to determine effect additional luminescent λ<sub>m</sub>=626nm on physical endurance in 12-week-old male mice. Four groups animals were used: Control I, intact animals; II, exposure standard artificial day light 5 B<sub>T/M2</sub>; III, solar absorbed...