D.A. Gorbachev
A5038797124- Particle physics theoretical and experimental studies
- High-Energy Particle Collisions Research
- Quantum Chromodynamics and Particle Interactions
- Advanced Fluorescence Microscopy Techniques
- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Particle Detector Development and Performance
- Photodynamic Therapy Research Studies
- Cancer Research and Treatments
- Superconducting Materials and Applications
- Photosynthetic Processes and Mechanisms
- Radiation Detection and Scintillator Technologies
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Luminescence and Fluorescent Materials
- Medical Imaging Techniques and Applications
- Genomics and Chromatin Dynamics
- Dark Matter and Cosmic Phenomena
- Ultrasound and Hyperthermia Applications
- Click Chemistry and Applications
- Advanced X-ray and CT Imaging
- PARP inhibition in cancer therapy
- Light effects on plants
- COVID-19 diagnosis using AI
- Coal Combustion and Slurry Processing
Institute of Bioorganic Chemistry
2015-2024
Planta
2024
Skolkovo Institute of Science and Technology
2019-2021
Bauman Moscow State Technical University
2019-2021
All-Russian Scientific Research Institute of Radio Engineering
2019
Lomonosov Moscow State University
2015-2017
Budker Institute of Nuclear Physics
1998-2008
Siberian Branch of the Russian Academy of Sciences
2008
Yale University
2004
Novosibirsk State University
2000-2003
The cross-section of the process e+e−→π+π− has been measured using about 11 4000 events collected by CMD-2 detector at VEPP-2M e+e− collider in center-of-mass energy range from 0.61 to 0.96 GeV. Results pion form factor determination with a 0.6% systematic uncertainty are presented. following values ρ- and ω-meson parameters were found: Mρ=(776.09±0.81) MeV, Γρ=(144.46±1.55) Γ(ρ→e+e−)=(6.86±0.12) keV, Br(ω→π+π−)=(1.33±0.25)%. Implications for hadronic contribution muon anomalous magnetic...
Abstract The discovery of the bioluminescence pathway in fungus Neonothopanus nambi enabled engineering eukaryotes with self-sustained luminescence. However, brightness luminescence heterologous hosts was limited by performance native fungal enzymes. Here we report optimized versions that enhance one to two orders magnitude plant, and mammalian hosts, enable longitudinal video-rate imaging.
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation cellular proteins. Here, we report an orange mutant red fluorescent protein KillerRed becomes toxic blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore is novel for photosensitizers. We show...
A genetically encoded fluorescent tag for live cell microscopy is presented. This composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative green (GFP)-like chromophore with red fluorescence. The reversible binding the fluorogen accompanied by three orders magnitude increase in fluorescence (580-650 nm). proposed dye instantly stains target cellular proteins fused FAST, washes out minute timescale, exhibits higher photostability signal confocal...
Abstract Epigenetic modifications (methylation, acetylation, etc.) of core histones play a key role in regulation gene expression. Thus, the epigenome changes strongly during various biological processes such as cell differentiation and dedifferentiation. Classical methods analysis epigenetic mass-spectrometry chromatin immuno-precipitation, work with fixed cells only. Here we present genetically encoded fluorescent probe, MPP8-Green, for detecting H3K9me3, histone modification associated...
The cross section of the process e+e−→π+π−π0 has been measured in c.m. energy range 984–1060 MeV with CMD-2 detector at VEPP-2M collider. obtained value Br(ϕ→e+e−)Br(ϕ→π+π−π0)=(4.51±0.16±0.11)×10−5 is good agreement previous measurements and best accuracy. Analysis Dalitz plot was performed. contributions dominant ϕ→ρπ mechanism as well a small direct ϕ→3π amplitude were determined.
Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation target proteins, DNA damage, and cell killing. Only two such GFP-based (FPs), KillerRed its monomeric variant SuperNova, were described up to date. Here, we present crystallographic study their orange successors, dimeric KillerOrange mKillerOrange, at 1.81 1.57 Å resolution, respectively. They are the first orange-emitting protein with...
The process $e^+e^- \to K^0_L K^0_S$ has been studied with the CMD-2 detector using about 950 events detected in center-of-mass energy range from 1.05 to 1.38 GeV. cross section exceeds expectation based on contributions of rho(770), omega(782) and phi(1020) mesons only.
The e+e- -> pi+pi-pi+pi- cross section has been measured using 5.8/pb of integrated luminosity collected with the CMD-2 detector at VEPP-2M collider. Analysis data a refined efficiency determination and use both three- four-track events allowed doubling sample reduction systematic errors to 5-7%.
We report the creation of a novel group ABDI‐BF 2 fluorescent dyes based on conformationally locked GFP chromophore. studied intramolecular mechanism radiationless deactivation fluorophore by introducing various substituents at nitrogen atom. The results this study and our previous work allowed us to claim that in case is determined formation nonfluorescent internal charge transfer exited state with planar quinoidal structure. electronic effects have greater impact than conformation. Thus,...
Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution this and identified SuperNova2, a with S10R substitution that results enhanced brightness, chromophore maturation phototoxicity bacterial mammalian...
In this paper, we propose a fluorescence-lifetime imaging microscopy (FLIM) multiplexing system based on the fluorogen-activating protein FAST. This genetically encoded fluorescent labeling platform employs FAST mutants that activate same fluorogen but provide different fluorescence lifetimes for each specific protein-dye pair. All proposed probes with varying possess nearly identical and smallest-in-class size, along quite similar steady-state optical properties. live mammalian cells,...