A5019088814- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hematological disorders and diagnostics
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- CAR-T cell therapy research
- Autoimmune Bullous Skin Diseases
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
Institute of Bioorganic Chemistry
2021-2025
Institute of Molecular and Cell Biology
2024-2025
Skolkovo Institute of Science and Technology
2021-2024
Pirogov Russian National Research Medical University
2024
Institute of Bioorganic Chemistry
2024
Abstract Epigenetic modifications (methylation, acetylation, etc.) of core histones play a key role in regulation gene expression. Thus, the epigenome changes strongly during various biological processes such as cell differentiation and dedifferentiation. Classical methods analysis epigenetic mass-spectrometry chromatin immuno-precipitation, work with fixed cells only. Here we present genetically encoded fluorescent probe, MPP8-Green, for detecting H3K9me3, histone modification associated...
Abstract Cancer immunotherapy is predominantly based on T cell–centric approaches. At the same time, adaptive immune response in tumor environment also includes clonally produced immunoglobulins and clonal effector/memory B cells that participate antigen-specific decisions through their interactions with cells. Here, we investigated role of infiltrating bladder cancer via patient dataset analysis intratumoral immunoglobulin repertoires. We showed IgG1/IgA ratio a prognostic indicator for...
Introduction The functional programs of CD4 + T helper (Th) cell clones play a central role in shaping immune responses to different challenges. While advances single-cell RNA sequencing (scRNA-Seq) have significantly improved our understanding the diversity Th cells, relationship between scRNA-Seq clusters and traditionally characterized subsets remains ambiguous. Methods In this study, we introduce TCR-Track, method leveraging repertoire data map phenotypically sorted onto profiles....
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a debilitating disease caused by impairments in the junctions dermis and basement membrane epidermis. Mutations COL7A1 gene induce multiple abnormalities, including chronic inflammation profibrotic changes skin. However, correlations between specific mutations their phenotypic output remain largely unexplored. gene, described here, were found DEB register. Among them, two homozygous cases compound heterozygous identified. We...
Abstract The functional programs chosen by B and T cell clones fundamentally determine the architecture of immune response to distinct challenges. Advances in scRNA-Seq have improved our understanding diversity stability these programs, but it has proven difficult link this information with known lymphocyte subsets. Here, we introduce Sort-Seq, an repertoire-based method that allows exact positioning phenotypically defined subsets within data. Sort-Seq outperformed CITE-Seq for accurate...
Single-cell RNA sequencing (scRNA-Seq) provides invaluable insights in cell biology. Current scRNA-Seq analytic approaches do not distinguish between spliced and unspliced mRNA. velocity paradigm suggests that the presence of mRNA reflects transitional states, informative for studies dynamic processes such as embryogenesis or tissue regeneration. Alternatively, stable subsets may also maintain reservoirs prompt initiation transcription-independent expression. Based on latter paradigm, we...
Abstract Our understanding of the heterogeneity tumor-infiltrating B cells (TIBs) evolves in parallel with advances high-dimensional flow cytometry and single-cell RNA sequencing (scRNA-seq). Subpopulations TIBs have been described diverse even opposite roles tumor control, based on surface molecule, cytokine transcriptional factor expression. Furthermore, recently predictive role IgA IgG expression tumors was shown for melanoma KRAS-mutated, but not KRAS wild type, lung adenocarcinoma. This...
<div>Abstract<p>Cancer immunotherapy is predominantly based on T cell–centric approaches. At the same time, adaptive immune response in tumor environment also includes clonally produced immunoglobulins and clonal effector/memory B cells that participate antigen-specific decisions through their interactions with cells. Here, we investigated role of infiltrating bladder cancer via patient dataset analysis intratumoral immunoglobulin repertoires. We showed IgG1/IgA ratio a...
Supplementary Data from Accounting for B-cell Behavior and Sampling Bias Predicts Anti–PD-L1 Response in Bladder Cancer
<div>Abstract<p>Cancer immunotherapy is predominantly based on T cell–centric approaches. At the same time, adaptive immune response in tumor environment also includes clonally produced immunoglobulins and clonal effector/memory B cells that participate antigen-specific decisions through their interactions with cells. Here, we investigated role of infiltrating bladder cancer via patient dataset analysis intratumoral immunoglobulin repertoires. We showed IgG1/IgA ratio a...
Supplementary Data from Accounting for B-cell Behavior and Sampling Bias Predicts Anti–PD-L1 Response in Bladder Cancer
ABSTRACT Tumor-infiltrating B cells and intratumorally-produced immunoglobulins (IG) play important roles in the tumor microenvironment response to immunotherapy 1–5 . IgG antibodies produced by intratumoral may drive antibody-dependent cellular cytotoxicity (ADCC) enhance antigen presentation dendritic 6–8 Furthermore, are efficient antigen-specific presenters that can essentially modulate behaviour of helper T 9–11 Here we investigated role IG isotype clonality bladder cancer. Our results...