A5090457409- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chromosomal and Genetic Variations
- Lymphoma Diagnosis and Treatment
- Genomics and Phylogenetic Studies
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Environmental DNA in Biodiversity Studies
- CAR-T cell therapy research
- Chronic Myeloid Leukemia Treatments
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Protist diversity and phylogeny
- Synthesis and Characterization of Heterocyclic Compounds
- Genomic variations and chromosomal abnormalities
- Mosquito-borne diseases and control
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- T-cell and Retrovirus Studies
- Epigenetics and DNA Methylation
- Organophosphorus compounds synthesis
- Synthesis of heterocyclic compounds
N.D. Zelinsky Institute of Organic Chemistry
2003-2024
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
2017-2023
Institute of Bioorganic Chemistry
2015-2023
Emirates Foundation
2023
Genomics (United Kingdom)
2022-2023
Abu Dhabi National Oil (United Arab Emirates)
2023
Ministry of Health of the Russian Federation
2022
Institute of Bioorganic Chemistry
2009-2020
Russian Academy of Sciences
2012-2013
Moscow State University of Fine Chemical Technologies
2009-2012
T cell receptor (TCR) repertoire data contain information about infections that could be used in disease diagnostics and vaccine development, but extracting remains a major challenge. Here we developed statistical framework to detect TCR clone proliferation contraction from longitudinal data. We applied this three pairs of identical twins immunized with the yellow fever vaccine. identified 600 1,700 responding TCRs each donor validated them using independent assays. While were mostly...
The fusion gene MLL/AF4 defines a high-risk subtype of pro-B acute lymphoblastic leukemia. Relapse can be associated with lineage switch from to myeloid leukemia, resulting in poor clinical outcomes caused by resistance chemotherapies and immunotherapies. In this study, the relapses shared oncogene breakpoints their matched lymphoid presentations originated various differentiation stages immature progenitors through committed B-cell precursors. Lineage switching is linked substantial changes...
Retroelement activity is a common source of polymorphisms in human genome. The mechanism whereby retroelements contribute to the intraindividual genetic heterogeneity by inserting into DNA somatic cells gaining increasing attention. Brain tissues are suspected accumulate as result activity. This study aims expand our understanding role play generating mosaicism neural tissues. Whole-genome Alu and L1 profiling genomic extracted from cerebellum, frontal cortex, subventricular zone, dentate...
We report incidence and deep molecular characteristics of lineage switch in 182 pediatric patients affected by B-cell precursor acute lymphoblastic leukemia (BCP-ALL), who were treated with blinatumomab. documented six cases that occurred after or during blinatumomab exposure. Therefore, conversion was found 17.4% all resistance (4/27) 3.2% relapses (2/63). Half switched completely from BCP-ALL to CD19-negative myeloid leukemia, others retained CD19-positive B-blasts acquired an additional...
High-throughput sequencing of adaptive immune receptor repertoires is a valuable tool for receiving insights in immunity studies. Several powerful TCR/BCR repertoire reconstruction and analysis methods have been developed the past decade. However, detecting correcting discrepancy between real experimentally observed lymphocyte clone frequencies are still challenging. Here, we discovered hallmark anomaly ratio read count count-based non-functional clonotypes multiplex PCR-based repertoires....
Introduction The functional programs of CD4 + T helper (Th) cell clones play a central role in shaping immune responses to different challenges. While advances single-cell RNA sequencing (scRNA-Seq) have significantly improved our understanding the diversity Th cells, relationship between scRNA-Seq clusters and traditionally characterized subsets remains ambiguous. Methods In this study, we introduce TCR-Track, method leveraging repertoire data map phenotypically sorted onto profiles....
We aimed to develop an antibody panel and data analysis algorithm for multicolor flow cytometry (MFC), which is a reliable method minimal residual disease (MRD) detection in patients with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) treated CD19-directed therapy. The development of the approach, was adapted case possible CD19 loss, based on additional B-lineage marker expression obtained from study primary BCP-ALL patients, immunophenotypic changes that occur during blinatumomab...
Abstract A straightforward, practical, and atom‐economical three‐component synthesis of phosphoryl thioamides from phosphinic chlorides, elemental sulfur, aliphatic amines has been developed. The scope limitations this transformation have investigated. This protocol is distinguished by metal‐ organic solvent‐free conditions, high tolerance to various functional groups; it can be adapted for scaled‐up synthesis. magnified image
The solvent-free sulfur-mediated reactions of phosphinic chlorides with alkyl diamines were developed for the practical synthesis unknown phosphoryl-substituted 4,5-dihydro-1H-imidazoles, 1,4,5,6-tetrahydropyrimidines, and thioamides. Their good tolerance to functional groups, broad substrate scope, easy scalability shown. chemoselective preparation a variety bis(thioamides) was accomplished via adjustment solvent.
Summary Rearrangements of T‐ and B‐cell receptor (TCR BCR) genes are useful markers for clonality assessment as well minimal residual disease (MRD) monitoring during the treatment haematological malignancies. Currently, rearrangements three out four TCR all BCR loci used this purpose. The fourth gene, TRA , has not been so far due to lack a method its rearrangement detection in genomic DNA. Here we propose first high‐throughput sequencing based identification clonal gene at DNA level. is on...
The development and implementation of vaccines have been growing exponentially, remaining one the major successes healthcare over last century. Nowadays, active regular immunizations prevent epidemics many viral diseases, including tick-borne encephalitis (TBE). Along with generation virus-specific antibodies, a highly effective vaccine should induce T cell responses providing long-term immune defense. In this study, we performed longitudinal high-throughput receptor (TCR) sequencing to...
Abstract Introduction B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) is the most common neoplasm in children. One of long known recurrent rearrangements BCP‐ALL t(1;19)(q23;p13.3)/ TCF3 :: PBX1 . However, other gene were also described that are associated with significant difference ALL prognosis. Methods The current study aimed to analyze spectrum children Russian Federation. A cohort 203 patients was selected based on FISH screening and studied by karyotyping, FISH, RT‐PCR high...
New 5-acetyl-4-alkylthio-6-methyl-2(1H)-pyrimidinethiones were prepared from diacetylketene N,S-acetals and isothiocyanates.They converted into 4-amino derivatives, which can be applied for the construction of functionalized pyrido [2,3-d]pyrimidines pyrimido [4,5d]pyrimidines.
T-cell receptor (TCR) repertoire data contain information about infections that could be used in disease diagnostics and vaccine development, but extracting remains a major challenge. Here we developed statistical framework to detect TCR clone proliferation contraction from longitudinal data. We applied this three pairs of identical twins immunized with the yellow fever vaccine. identified 500-1500 responding TCRs each donor validated them using independent assays. While were mostly private,...
T-cell receptor (TR) diversity of the variable domains is generated by recombination both alpha (TRA) and beta (TRB) chains. The textbook process TRB chain production starts with TRBD TRBJ gene rearrangement, followed rearrangement a TRBV to partially rearranged D-J gene. Unsuccessful V-D-J rearrangements lead apoptosis cell. Here, we performed deep sequencing poorly explored pool partial TRBD1-TRBD2 in genomic DNA. We reconstructed full repertoires human using novel validated them detecting...
Simultaneous multilineage hematologic malignancies are uncommon and associated with poorer prognosis than single-lineage leukemia or lymphoma. Here, we describe a concomitant malignant neoplasm in 4-year-old boy. The child presented massive lymphoproliferative syndrome, nasal breathing difficulties, snoring. Morphological, immunocytochemical, flow cytometry diagnostics showed coexistence of acute myeloid (AML) peripheral T-cell lymphoma (PTCL). Molecular examination revealed rare...
There is increasing evidence that the transpositional activity of retroelements (REs) not limited to germ line cells, but often occurs in tumor and normal somatic cells. Somatic transpositions were found several human tissues are especially typical for brain. Several computational experimental approaches detection retroelement insertions was developed past few years. These successfully applied detect clonally expanded At same time, identification presented small proportion such as neurons,...
Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts hematological malignancies, such acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia low insertional activity. Acute lymphoblastic (ALL) adults childhood leukemias not studied this context. We performed search for new insertions (Alu L1) 44 ALL, 14 adult ALL...
Acute lymphoblastic leukemia (ALL) with translocation t(17;19)(q21-q22;p13) TCF3::HLF (E2A::HLF) accounts for less than 1% of childhood B-lineage ALL. Since the first description, patients this type ALL are stratified into high-risk group. The disease often has a unique clinical presentation disseminated intravascular coagulation and hypercalcemia, that uncommon in other types This is characterized by an extremely poor prognosis despite intensive treatment hematopoietic stem cell...
Abstract Background Retroelements (REs) occupy a significant part of all eukaryotic genomes including humans. The majority retroelements in the human genome are inactive and unable to retrotranspose. Dozens active copies repressed most normal tissues by various cellular mechanisms. These can become germline brain or cancer, leading new retroposition events. consequences such events their role cell functioning carcinogenesis not yet fully understood. If insertions occur small portion cells...
Abstract The COVID-19 pandemic offers a powerful opportunity to develop methods for monitoring the spread of infectious diseases based on their signatures in population immunity. Adaptive immune receptor repertoire sequencing (AIRR-seq) has become method choice identifying T cell (TCR) biomarkers encoding pathogen specificity and immunological memory. AIRR-seq can detect imprints past ongoing infections facilitate study individual responses SARS-CoV-2, as shown many recent studies. Here, we...