A5026201343- Cancer, Hypoxia, and Metabolism
- Adrenal and Paraganglionic Tumors
- Pituitary Gland Disorders and Treatments
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- Plant Micronutrient Interactions and Effects
- Cancer, Lipids, and Metabolism
- Plant Molecular Biology Research
- RNA modifications and cancer
- Gut microbiota and health
- Agricultural Science and Fertilization
- Rice Cultivation and Yield Improvement
- Genomics and Phylogenetic Studies
- Genetics, Aging, and Longevity in Model Organisms
- Colorectal Cancer Treatments and Studies
- Diet and metabolism studies
- Phytoestrogen effects and research
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Liver Disease and Transplantation
- Genetic factors in colorectal cancer
Engelhardt Institute of Molecular Biology
2016-2025
Ulyanovsk State University
2024
Independent University of Moscow
2024
National Medical Research Center of Cardiology
2016-2019
P.A. Hertzen Moscow Oncology Research Institute
2014-2019
Ministry of Health of the Russian Federation
2014-2019
Moscow Institute of Physics and Technology
2015-2017
Russian Cancer Research Center NN Blokhin
2016
Russian Academy of Sciences
1992-2014
St Petersburg University
2013
Activated sludge (AS) plays a crucial role in the treatment of domestic and industrial wastewater. AS is biocenosis microorganisms capable degrading various pollutants, including organic compounds, toxicants, xenobiotics. We performed 16S rRNA gene sequencing incoming sewage three wastewater plants (WWTPs) responsible for processing with different origins: municipal wastewater, slaughterhouse refinery sewage. In contrast to taxonomic structure was found become stable time, each WWTP...
The molecular mechanisms that determine the organism's response to a variety of doses and modalities stress factors are not well understood. We studied effects ionizing radiation (144, 360 864 Gy), entomopathogenic fungus (10 100 CFU), starvation (16 h), cold shock (+4, 0 -4°C) on an viability indicators (survival locomotor activity) transcriptome changes in Drosophila melanogaster model. All but resulted decrease lifespan proportional dose treatment. However, stress-factors affected...
Gut dysbiosis and subclinical intestinal damage are common in cirrhosis. The aim of this study was to examine the association biomarkers (diamine oxidase [DAO], claudin 3, fatty acid binding protein [I-FABP; FABP2]) with state gut microbiota blood levels DAO were inversely correlated lipopolysaccharide (LPS), presepsin, TNF-α, severity cirrhosis according Child–Pugh scores. level I-FABP directly 3 but not that DAO. Patients small bacterial overgrowth (SIBO) had lower than patients without...
Abstract Sestrin1 and Sestrin2 (SESN1&2) are evolutionarily conserved, stress-responsive proteins that regulate cell growth viability. The primary target of Sestrins is the mTORC1 protein kinase, an activator anabolic processes autophagy inhibitor. Our previous studies showed inactivating SESN1&2 in lung adenocarcinoma A549 cells accelerates proliferation confers resistance to death without affecting activity, suggesting modulate cellular via mTORC1-independent mechanisms. This work...
CHL1 gene (also known as CALL) on 3p26.3 encodes a one-pass trans-membrane cell adhesion molecule (CAM). Previously CAMs of this type, including L1, were shown to be involved in cancer growth and metastasis.We used Clontech Cancer Profiling Arrays (19 different types cancers, 395 samples) analyze expression the gene. The results further validated by RT-qPCR for breast, renal lung cancer. revealed differential gene: down-regulation/silencing majority primary tumors up-regulation associated...
This study aimed to clarify genetic and epigenetic alterations that occur during lung carcinogenesis design perspective sets of newly identified biomarkers. The original method includes chromosome 3 specific NotI-microarrays containing 180 NotI clones associated with genes for hybridization 40 paired normal/tumor DNA samples primary tumors: 28 squamous cell carcinomas (SCC) 12 adenocarcinomas (ADC). NotI-microarray data were confirmed by qPCR bisulfite sequencing analyses. Forty-four showed...
Colorectal cancer is one of the most common cancers in world. It well known that chronic inflammation can promote progression colorectal (CRC). Recently, a number studies revealed potential association between inflammation, progression, and infection caused by enterotoxigenic Bacteroides fragilis (ETBF). Bacterial enterotoxin activates spermine oxidase (SMO), which produces spermidine H2O2 as byproducts polyamine catabolism, which, turn, enhances tissue injury. Using qPCR analysis, we...
The fundamental mission of the Chromosome-Centric Human Proteome Project (C-HPP) is research human proteome diversity, including rare variants. Liver tissues, HepG2 cells, and plasma were selected as one major objects for C-HPP studies. proteogenomic approach, a recently introduced technique, powerful method predicting validating proteoforms coming from alternative splicing, mutations, transcript editing. We developed PPLine, Python-based pipeline providing automated single-amino-acid...
DNA double-strand breaks (DSBs) are involved in many cellular mechanisms, including replication, transcription, and genome rearrangements. The recent observation that hot spots of DSBs human chromosomes delimit domains possess coordinately expressed genes suggests a strong relationship between the organization transcription patterns DSBs. In this study, we performed mapping 43-kb ribosomal (rDNA) repeated unit. We observed rDNA units corresponded to most fragile sites these possessed at...
Genetic and epigenetic alterations in cervical carcinomas were investigated using NotI-microarrays containing 180 cloned sequences flanking all NotI-sites associated with genes on chromosome 3. In total, 48 paired normal/tumor DNA samples, specifically enriched NotI-sites, hybridized to NotI-microarrays. Thirty genes, including tumor suppressors or candidates (for example, VHL, RBSP3/CTDSPL, ITGA9, LRRC3B, ALDH1L1, EPHB1) previously unknown as cancer-associated (ABHD5, C3orf77, PRL32,...
Flax (Linum usitatissimum L.) is a crop plant used for fiber and oil production. Although potentially high-yielding flax varieties have been developed, environmental stresses markedly decrease Among biotic stresses, Fusarium oxysporum f. sp. lini recognized as one of the most devastating pathogens. It causes wilt disease that major limiting factors production worldwide. Breeding cultivation resistant to F. effective method controlling disease. mechanisms response actively studied, data on...
Colorectal cancer (CRC) is a common worldwide. The main cause of death in CRC includes tumor progression and metastasis. At molecular level, these processes may be triggered by epithelial-mesenchymal transition (EMT) necessitates specific alterations cell metabolism. Although several EMT-related metabolic changes have been described CRC, the mechanism still poorly understood. Using CrossHub software, we analyzed RNA-Seq expression profile data derived from Cancer Genome Atlas (TCGA) project....
The modulation of longevity genes and aging-associated signaling pathways using pharmacological agents is one the potential ways to prolong lifespan increase vitality an organism. Phytochemicals flavonoids non-steroidal anti-inflammatory drugs have a large as geroprotectors. goal present study was investigate effects long-term short-term consumption quercetin, (-)-epicatechin, ibuprofen on lifespan, resistance stress factors (paraquat, hyperthermia, γ-radiation, starvation), well...
About 30% of the world's ice-free land area is occupied by acid soils. In soils with pH below 5, aluminum (Al) releases to soil solution and becomes highly toxic for plants. Therefore, breeding varieties that are resistant Al needed. Flax (Linum usitatissimum L.) grown worldwide fiber seed production. toxicity in a serious problem flax cultivation. However, very little known about mechanisms resistance genetics this resistance. present work, we sequenced 16 transcriptomes cultivars (Hermes...
The SEMA3B gene is located in the 3p21.3 LUCA region, which frequently affected different types of cancer. objective our study was to expand knowledge as a tumor suppressor and mechanisms its inactivation. In this study, several experimental approaches were used: growth analyses apoptosis assays vitro SCID mice, expression methylation other. With use small cell lung cancer line U2020 we confirmed function suppressor, showed that suppression can be realized through induction and, possibly,...
This study aimed to clarify epigenetic and genetic alterations that occur during renal carcinogenesis. The original method includes chromosome 3 specific NotI-microarrays containing 180 NotI-clones associated with 188 genes for hybridization 23 paired normal/tumor DNA samples of primary clear cell carcinomas (ccRCC). Twenty-two showed methylation and/or deletion in 17–57% tumors. These include tumor suppressors or candidates ( VHL, CTDSPL, LRRC3B, ALDH1L1 , EPHB1 ) were not previously...