A5052011893- Glycosylation and Glycoproteins Research
- Occupational and environmental lung diseases
- Epigenetics and DNA Methylation
- Salivary Gland Tumors Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Chromosomal and Genetic Variations
- Medical Imaging and Pathology Studies
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Head and Neck Surgical Oncology
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Genetic Mapping and Diversity in Plants and Animals
- Drug Transport and Resistance Mechanisms
- Renal cell carcinoma treatment
- Hedgehog Signaling Pathway Studies
- Renal and related cancers
- Polyamine Metabolism and Applications
- Bone and Dental Protein Studies
- Molecular Biology Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Research and Splicing
Vanderbilt University Medical Center
2011-2023
Vanderbilt University
2010-2023
Kemerovo State University
2023
Kuban State Agrarian University
2022
Yale University
2003-2017
Yale Cancer Center
2012-2013
Science for Life Laboratory
2012
Stockholm University
2012
Karolinska Institutet
2012
The University of Texas MD Anderson Cancer Center
2012
To discover genes involved in von Hippel-Lindau (VHL)-mediated carcinogenesis, we used renal cell carcinoma lines stably transfected with wild-type VHL-expressing transgenes. Large-scale RNA differential display technology applied to these identified several differentially expressed genes, including an alpha carbonic anhydrase gene, termed CA12 . The deduced protein sequence was classified as a one-pass transmembrane CA possessing apparently intact catalytic domain the extracellular module....
The inability to forecast outcomes for malignant mesothelioma prevents clinicians from providing aggressive multimodality therapy the most appropriate individuals who may benefit such an approach. We investigated whether specific microRNAs (miR) could segregate a largely surgically treated group of mesotheliomas into good or bad prognosis categories. A training set 44 and test 98 tumors were analyzed by custom miR platform, along with 9 cell lines 3 normal mesothelial lines. Functional...
The human genome encodes several hundred microRNA (miRNA) genes that produce small (21–23n) single strand regulatory RNA molecules. Although abnormal expression of miRNAs has been linked to cancer progression, the mechanisms this dysregulation are poorly understood. Malignant mesothelioma (MM) pleura is an aggressive and highly lethal resistant conventional therapies. We others previously loss 9p21.3 chromosome in MM with short time tumor recurrence. In study, we report cell lines derived...
CHL1 gene (also known as CALL) on 3p26.3 encodes a one-pass trans-membrane cell adhesion molecule (CAM). Previously CAMs of this type, including L1, were shown to be involved in cancer growth and metastasis.We used Clontech Cancer Profiling Arrays (19 different types cancers, 395 samples) analyze expression the gene. The results further validated by RT-qPCR for breast, renal lung cancer. revealed differential gene: down-regulation/silencing majority primary tumors up-regulation associated...
Abstract Purpose: Human papillomavirus (HPV) is linked with a subset of head and neck squamous cell carcinomas (HNSCC). HPV-positive HNSCCs show better prognosis than HPV-negative HNSCCs, which may be explained by sensitivity the to ionizing radiation (IR). Although molecular mechanism behind IR in unresolved, DNA damage response (DDR) might significant determinant sensitivity. An important player DDR, SMG-1 (suppressor morphogenetic effect on genitalia), potential tumor suppressor therefore...
It has been previously suggested that overexpression of mitochondrial superoxide dismutase (SOD) attenuates cancer development; however, the exact mechanism remains unclear. In this work, we have studied direct effect mitochondria-targeted scavenger, (2-(2,2,6,6-tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride (mitoTEMPO), on B16-F0 mouse melanoma cells and tumor growth in a nude model human melanoma. We show scavenging inhibited cell growth, reduced viability,...
Salivary adenoid cystic carcinoma (ACC) is an insidious slow-growing cancer with the propensity to recur and metastasise distant sites. Basal-like breast (BBC) a molecular subtype that constitutes 15–20% of cancers, shares histological similarities basal cell markers ACC, lacks expression ER (oestrogen receptor), PR (progesterone HER2 (human epidermal growth factor receptor 2), and, similar metastasises predominantly lung brain. Both cancers lack targeted therapies owing poor understanding...
In this study, we focus on different modes of regulation STRA13, a human ortholog the mouse basic helix-loop-helix transcriptional factor, previously identified by us as new von Hippel-Lindau tumor suppressor gene (VHL) target. The was overexpressed in VHL-deficient cell lines and tumors, specifically clear renal carcinomas hemangioblastomas. Introduction wild type VHL transgene into carcinoma restored low level expression STRA13. Overexpression also detected many common malignancies with an...
Malignant brain tumors exhibit distinct metabolic characteristics. Despite high levels of lactate, the intracellular pH is more alkaline than normal brain. Additionally, with increasing malignancy, display intratumoral hypoxia. Carbonic anhydrase (CA) IX and XII are transmembrane isoenzymes that induced by tissue They participate in regulation homeostasis catalyzing reversible hydration carbon dioxide. The aim our study was to investigate whether different histology grade malignancy express...
Although the existence of cancer stem cells (CSC) in adenoid cystic carcinoma (ACC) has been proposed, lack assays for their propagation and uncertainty about molecular markers prevented characterization. Our objective was to isolate CSC from ACC provide insight into signaling pathways that support propagation.To characterize them, we used ROCK inhibitor-supplemented cell culture, immunomagnetic sorting, andin vitro/in vivoassays viability tumorigenicity.We identified CD133-positive...
Abstract Pleural malignant mesothelioma (MM) is an aggressive cancer with a very long latency and short median survival. Little known about the genetic events that trigger MM their relation to poor outcome. The goal of our study was characterize major genomic gains losses associated origin progression assess clinical significance. We performed Representative Oligonucleotide Microarray Analysis (ROMA) on DNA isolated from tumors 22 patients who recurred at variable interval disease after...
Abstract Exposure of human lung cells to carcinogenic nickel compounds in the presence oxygen up‐regulated carbonic anhydrase IX (CA IX) and NDRG1/Cap43, both known as intrinsic hypoxia markers cancer‐associated genes. This suggests that factors other than a shortage may be involved this induction. Both proteins can also induced by culturing these high density without medium change. The intracellular ascorbate measurements revealed its rapid depletion metal‐ density‐exposed cells. Nickel...
Hypoxia-inducible factor (HIF-1) regulates the expression of genes that facilitate tumor cell survival by making them more resistant to therapeutic intervention. Recent evidence suggests activation other transcription factors, in cooperation with HIF-1 or acting alone, is involved upregulation hypoxia-inducible genes. Here we report high density, a condition might mimic physiologic situation growing and most probably representing nutritional starvation, upregulates This can occur...
Fus1 has been established as mitochondrial tumor suppressor, immunomodulator, and antioxidant protein, but molecular mechanism of these activities remained to be identified. Based on putative calcium-binding myristoyl-binding domains that we identified in Fus1, explored our hypothesis regulates calcium handling calcium-coupled processes.Fus1 loss resulted reduced rate uptake calcium-loaded epithelial cells, splenocytes, activated CD4(+) T cells. The Fus1-deficient cells correlated with...
Bromine and peroxidasin (an extracellular peroxidase) are essential for generating sulfilimine cross-links between a methionine hydroxylysine within collagen IV, basement membrane protein. The increase the structural integrity of membranes. formation depends on ability to use bromide hydrogen peroxide substrates produce hypobromous acid (HOBr). Once cross-link is created, released into space becomes available reutilization. Whether HOBr generated by used very selectively creating or whether...