A5036198815- Electron Spin Resonance Studies
- Synthesis and Reactions of Organic Compounds
- Synthesis and Characterization of Heterocyclic Compounds
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Synthesis and Biological Evaluation
- Magnetism in coordination complexes
- Fluorine in Organic Chemistry
- Lanthanide and Transition Metal Complexes
- Free Radicals and Antioxidants
- Synthesis of heterocyclic compounds
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Radical Photochemical Reactions
- Analytical Chemistry and Sensors
- Synthesis and biological activity
- Photochemistry and Electron Transfer Studies
- Chemical Reaction Mechanisms
- Organic Chemistry Cycloaddition Reactions
- Oxidative Organic Chemistry Reactions
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Chemical Reactions and Mechanisms
- Organophosphorus compounds synthesis
- Electrochemical Analysis and Applications
- Spectroscopy and Quantum Chemical Studies
Novosibirsk Institute of Organic Chemistry
2009-2021
Russian Federal Centre of Forensic Science of the Ministry of Justice of the Russian Federation
2018-2020
Siberian Branch of the Russian Academy of Sciences
1999-2017
Meshalkin National Medical Research Center
2014-2017
Russian Academy of Sciences
2002-2015
Scientific Research Institute of Biochemistry
2015
Nikolaev Institute of Inorganic Chemistry
2007-2010
Novosibirsk State University
2003-2010
International Tomography Center
2010
North Carolina State University
2004-2008
Superoxide (O2•−) has been implicated in the pathogenesis of many human diseases, but detection O2•− radicals biological systems is limited due to inefficiency spin trapping and lack site-specific information. This work studied production extracellular, intracellular mitochondrial neutrophils, cultured endothelial cells isolated mitochondria using a new set cationic, anionic neutral hydroxylamine probes with various lipophilicity cell permeability. Cyclic hydroxylamines rapidly react O2•−,...
It has been previously suggested that overexpression of mitochondrial superoxide dismutase (SOD) attenuates cancer development; however, the exact mechanism remains unclear. In this work, we have studied direct effect mitochondria-targeted scavenger, (2-(2,2,6,6-tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride (mitoTEMPO), on B16-F0 mouse melanoma cells and tumor growth in a nude model human melanoma. We show scavenging inhibited cell growth, reduced viability,...
Approach for in vivo real-time assessment of tumor tissue extracellular pH (pH(e)), redox, and intracellular glutathione based on L-band EPR spectroscopy using dual function redox nitroxide probe disulfide biradical, is described. These parameters were monitored PyMT mice bearing breast cancer tumors during treatment with granulocyte macrophage colony-stimulating factor. It was observed that pH(e) about 0.4 units lower than normal mammary gland tissue. Treatment factor decreased the value by...
The nitroxides of 7-azadispiro[5.1.5.2]pentadecane and 7-azadispiro[5.1.5.2]pentadeca-14-ene series have been prepared, including thiol-specific methane thiosulfonate spin label for site-directed labeling. effect spirocyclohexane moieties on chemical spectral properties has studied. obtained temperature dependencies electron relaxation parameters demonstrate that new may be suitable PELDOR distance measurements at 80–120 K. Moreover, the demonstrated much higher stability toward reduction by...
The synthesis of 2,2,5,5-tetraethylimidazole nitroxides from 3-ethylpent-2-ene is described. newly synthesized nitroxides, namely 4-methyl-2,2,5,5-tetraethyl-2,5-dihydro-1H-imidazol-1-yloxy (1), 3,4-dimethyl-2,2,5,5-tetraethylperhydroimidazol-1-yloxy (2) and 2,2,5,5-tetraethyl-4-pyrrolidin-1-yl-2,5-dihydro-1H-imidazol-1-oxyl (3), were found to be pH sensitive spin probes, with pK values 1.2, 4.95 7.4, respectively. most important finding was the fact that these new 20–30 times more stable in...
A variable radio frequency proton–electron double-resonance imaging (VRF PEDRI) approach for pH mapping of aqueous samples has been recently developed (Efimova et al. J. Magn. Reson. 2011, 209, 227−232). map is extracted from two PEDRI acquisitions performed at electron paramagnetic resonance (EPR) frequencies protonated and unprotonated forms a pH-sensitive probe. To translate VRF to an in vivo setting, advanced probe was synthesized. Probe deuteration resulted narrow spectral line 1.2 G...
In the last decade, joint efforts of biologists, chemists and physicists have helped in understanding dominant factors determining specificity directionality transmembrane transfer processes proteins. this endeavor, electron paramagnetic resonance (EPR) spectroscopy has played an important role. Characteristic examples such are hydrogen-bonding patterns polarity effects microenvironment protein sites involved process. These may undergo characteristic changes during reaction and, thereby,...
A series of 4-dialkylamino-2,5-dihydroimidazole nitroxides with pyridine-4-yl, 4-dimethylaminophenyl or 4-hydroxyphenyl groups in position 2 the imidazole ring were prepared using reaction RMgBr corresponding 5-dialkylamino-4,4-dimethyl-4H-imidazole 3-oxides. The EPR spectra shown to be pH-sensitive due consecutive protonation amidino moiety and basic group(s) at ring. 5,5-dimethyl-4-(dimethylamino)-2-ethyl-2-pyridine-4-yl-2,5-dihydro-1H-imidazol-1-oxyl showed a monotonic increase isotropic...
A sterically hindered bis-spirocyclic C(2)-symmetric chiral pyrrolidine-type nitroxide has been successfully synthesized starting from an l-tartaric derived nitrone. Starting a pyrrolidine flanked by two methylene groups, complete quaternization of the α-carbon atoms accomplished through iteration completely regio- and stereoselective intramolecular cycloaddition reactions organometallic additions to key nitrone intermediates, formed in turn oxidation procedures. This method appears be very...
Spin trapping compounds are used frequently to detect free radicals released by cells. Their cytotoxicity has be considered in order prevent perturbations of normal cell growth and viability. Eleven spin traps (eight nitrones three nitroso traps) have been tested for their effects on bovine aortic endothelial cells (toxicity range, 50% survival rate). The lowest was found 5,5‐dimethylpyrroline‐1‐oxide 2,2,4‐trimethyl‐2 H ‐imidazole‐1‐oxide whereas nitrosobenzene 2‐methyl‐2‐nitrosopropane...
The oxidant properties of the series 2,2,5,5-tetraalkyl imidazoline and imidazolidine nitroxides were investigated. An increase in number bulky alkyl substituents leads to a decrease rate reduction with ascorbate, which makes electrochemical potential more negative shifts equilibrium mixture nitroxide reference hydroxylamine (3-carboxy-1-hydroxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl-1-(15)N) toward starting compounds. effect structural factors on these reactions was analyzed by means...
This communication describes the use of a methanethiosulfonate derivative an imidazolidine nitroxide, methanethiosulfonic acid S-(1-oxyl-2,2,3,5,5-pentamethyl-imidazolidin-4-ylmethyl) ester, IMTSL, for site-directed pKa determination peptides by electron paramagnetic resonance. spin label is covalently attached to thiol group unique cysteines incorporated into peptide structures. The tertiary amine nitrogen N3 readily participates in proton exchange reactions, which are monitored through...