A5087357302- Ichthyology and Marine Biology
- Atmospheric chemistry and aerosols
- Air Quality and Health Impacts
- Fish Ecology and Management Studies
- Atmospheric Ozone and Climate
- Design Education and Practice
- Atmospheric aerosols and clouds
- Earthquake and Disaster Impact Studies
- Fish Biology and Ecology Studies
- Genomics and Phylogenetic Studies
- Usability and User Interface Design
- Marine animal studies overview
- Erosion and Abrasive Machining
- Atmospheric and Environmental Gas Dynamics
- Fish biology, ecology, and behavior
- Identification and Quantification in Food
- Isotope Analysis in Ecology
- Cardiac Valve Diseases and Treatments
- Environmental DNA in Biodiversity Studies
- Context-Aware Activity Recognition Systems
- Vehicle emissions and performance
- Cardiovascular Conditions and Treatments
- Air Quality Monitoring and Forecasting
- Mobile Health and mHealth Applications
- Paleontology and Evolutionary Biology
Okinawa Churashima Foundation
2015-2024
Nippon Steel (Japan)
2024
Senshu University
2013-2024
Asia Center for Air Pollution Research
2012-2023
Illinois Institute of Technology
2012-2023
University of Kochi
2023
Ochanomizu University
2023
National Institute of Biomedical Innovation, Health and Nutrition
2023
Niigata University
1986-2022
Saga University
1987-2022
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences 880 species, supplemented by partial mitogenome 160 elasmobranchs (sharks and rays). The target hypervariable region the 12S rRNA gene (163-185 bp), which contains sufficient information to identify fishes taxonomic family, genus species except some closely related congeners. To test versatility...
Abstract The profound changes in global SO 2 emissions over the last decades have affected atmospheric composition on a regional and scale with large impact air quality, deposition radiative forcing of sulfate aerosols. Reproduction historical pollution levels based aerosol models emission is crucial to prove that such are able predict future scenarios. Here, we analyze consistency trends observations sulfur components precipitation from major networks estimates six different 1990 until...
Modern cartilaginous fishes are divided into elasmobranchs (sharks, rays and skates) chimaeras, the lack of established whole-genome sequences for former has prevented our understanding early vertebrate evolution unique phenotypes elasmobranchs. Here we present de novo assemblies brownbanded bamboo shark cloudy catshark an improved assembly whale genome. These relatively large genomes (3.8-6.7 Gbp) contain sparse distributions coding genes regulatory elements exhibit reduced molecular...
Abstract Terrestrial animals must have frequent contact with water to survive, implying that environmental DNA ( eDNA ) originating from those should be detectable places containing in terrestrial ecosystems. Aiming detect the presence of mammals using forest samples, we applied a set universal PCR primers (MiMammal, modified version fish primers) for metabarcoding mammalian . The versatility MiMammal was tested silico and by amplifying s extracted tissues. results suggested are capable...
Abrasion tests were conducted using a brake dynamometer to determine the antimony (Sb) emission factor originating from automobiles. dusts commercially available pads (nonasbestos organic type) emitted into an enclosed chamber under various braking conditions in terms of initial driving speed and deceleration. Suspended inside collected on quartz fiber filter weighed. From experimental data, dust could be regressed as function kinetic energy loading time. Using regression function, factors...
Abstract Environmental DNA (eDNA) metabarcoding is a recently developed method to assess biodiversity based on high-throughput parallel sequencing applied present in the ecosystem. Although eDNA enables rapid assessment of biodiversity, it prone species detection errors that may occur at sequential steps field sampling, laboratory experiments, and bioinformatics. In this study, we illustrate how error rates metabarcoding-based can be accounted for by applying multispecies occupancy modelling...
Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most these employed disk fiber filters collect eDNA water samples, although number microbial in aquatic environments have filter cartridges, because cartridge has advantage accommodating large volumes and overall ease use. Here we provide protocol for filtration samples using extraction without having cut open housing. The main portions this consists 1) (water ≤4 L...
Air pollution is becoming a prominent social problem in fast-growing Asian economies. Taking the Bangkok Metropolitan Region (BMR) as case, we conducted an observational study of fine particulate matter (PM2.5) and acid deposition, consisting their continuous monitoring at two sites. To find major contributing sources PM2.5, PM composition data were analyzed by receptor modeling approach while load from BMR to air was characterized emission inventory. Our show generally alarming levels PM2.5...
Abstract Brassica self-incompatibility (SI) is controlled by SLG and SRK expressed in the stigma SP11/SCR anther. We determined sequences of S domains 36 alleles, 13 14 SP11 alleles from oleracea B. rapa. found three haplotypes lacking genes rapa, confirming that not essential for SI recognition system. Together with reported sequences, nucleotide diversities per synonymous nonsynonymous site (πS πN) at SRK, SLG, loci within were computed. The ratios πN:πS hypervariable region significantly...
APM was collected and trace elements existing in the particles were monitored since May 1995 this study. sample separately by size (d < 2 μm, 2–11 μm >11 μm) on roof of university building (45 m above ground) campus Faculty Science Engineering, Chuo University, Tokyo, Japan, using an Anderson low volume air sampler. The digested HNO3, H2O2 HF a microwave oven, major (Na, Mg, Al, K, Ca Fe) measured ICP-AES, (Li, Be, Ti, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Sb, Ba Pb) ICP-MS. It observed...
The emission, concentration levels, and transboundary transport of particulate polycyclic aromatic hydrocarbons (PAHs) in Northeast Asia were investigated using PAH measurements, the newly developed emission inventory (Regional Emission ASia for Persistent Organic Pollutants version, REAS-POP), chemical model Air Quality Model ver2 POPs RAQM2-POP). simulated concentrations nine PAHs agreed well with measured concentrations, results firmly established efficacy REAS/RAQM2-POP. It was found...
Abstract. The Model Inter-Comparison Study for Asia (MICS-Asia) phase III was conducted to promote understanding of regional air quality and climate change in Asia, which have received growing attention due the huge amount anthropogenic emissions worldwide. This study provides an overview acid deposition. Specifically, dry wet deposition following species analyzed: S (sulfate aerosol, sulfur dioxide (SO2), sulfuric (H2SO4)), N (nitrate nitrogen monoxide (NO), (NO2), nitric (HNO3)), A...
The Headquarters for Earthquake Research Promotion was established in the wake of devastating 1995 Hanshin Awaji earthquake with a mission to compile and statistically analyze vast amounts seismic data order make long-range assessments likelihood major earthquakes future. agency has reported that within next 30 years, Nankai region 60% probability magnitude (M) 8.4 earthquake, Tōnankai 60–70% chance M8.1 Tokyo metropolitan area 70% M7 earthquake. Tōkai faces most alarming odds an 87% M8...
Abstract. Land use in a catchment area has significant impacts on nitrate eluted from the catchment, including atmospheric deposited onto and remineralised produced within area. Although stable isotopic compositions of can be useful tracer to quantify land influences sources behaviour nitrate, it is best determine these for portion separately unprocessed obtain more accurate precise quantification influences. In this study, we determined spatial distribution seasonal variation than 30...
Genomic studies of vertebrate chromosome evolution have long been hindered by the scarcity chromosome-scale DNA sequences some key taxa. One those limiting taxa has elasmobranchs (sharks and rays), which harbor species often with numerous chromosomes enlarged genomes. Here, we report genome assembly for zebra shark Stegostoma tigrinum, an endangered that a relatively small among sharks (3.71 Gb), as well whale Rhincodon typus Our analysis using male-female comparison identified X Chromosome,...
Developing usable and desirable products requires an understanding of how users build close relationships with objects these can be controlled by developers. This paper discusses the importance concept affordances as instrument useful for between technical functions user tasks. The approach introduces a Function-Task Design Matrix to link tasks capture relevant affordance-level requirements throughout product architecture generation. Functional Operational Affordance levels are introduced...
Abstract. A new aerosol chemical transport model, the Regional Air Quality Model 2 (RAQM2), was developed to simulate Asian air quality. We implemented a simple version of triple-moment modal dynamics model (MADMS) and achieved completely dynamic (non-equilibrium) solution gas-to-particle mass transfer over wide range diameters from 1 nm super-μm. To consider variety atmospheric properties, category approach utilized in which aerosols were distributed into four categories: particles Aitken...
Atmospheric emissions of reactive nitrogen have increased significantly on a global scale due to increases the use artificial fertilizer and burning fossil fuels. The Asian region has been identified as high-risk area for deposition effects ecosystems. This paper describes measurement-based assessment carried out in cooperation with Acid Deposition Monitoring Network East Asia (EANET). investigation aimed understand status variability dry, wet total oxidized reduced over 10-year period...
Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most these employed disk fiber filters collect eDNA water samples, although number microbial in aquatic environments have filter cartridges, because cartridge has advantage accommodating large volumes and overall ease use. Here we provide protocol for filtration samples using extraction without having cut open housing. The main portions this consists 1) (water ≤4 L...