A5021951075- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Advanced ceramic materials synthesis
- Inflammatory Bowel Disease
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Liver Disease Diagnosis and Treatment
- Trauma Management and Diagnosis
- RNA modifications and cancer
- Thermal and Kinetic Analysis
- Receptor Mechanisms and Signaling
- Cancer Research and Treatments
- Pancreatic function and diabetes
- Amino Acid Enzymes and Metabolism
- Biochemical Analysis and Sensing Techniques
- Inorganic Chemistry and Materials
- Vitamin C and Antioxidants Research
- Protein Kinase Regulation and GTPase Signaling
- Thermal properties of materials
- Heat shock proteins research
- Psoriasis: Treatment and Pathogenesis
- Microwave Dielectric Ceramics Synthesis
- Organic and Molecular Conductors Research
Tohoku University
2010-2025
Jichi Medical University
2024
Fujita Health University
2024
Fujita Health University Hospital
2024
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
Jichi Medical University Saitama Medical Center
2024
Janssen (United States)
2019
Sendai University
2014-2018
Graduate School USA
2014-2018
The role of calcium in cell injury has been the subject much recent investigation. movement and redistribution this cation from extra to intracellular compartments shifts between may well play a determinate cell's reaction injury. Therefore, data such their correlation with morphological, biochemical cytoskeletal studies will provide better understanding these processes. To study effects regulation on acute lethal anoxic inhibition respiration cyanide, three experimental systems were...
We have developed a new fluorescent sensing probe for double-stranded RNA (dsRNA) by integrating thiazole orange (TO) as base surrogate into triplex-forming PNA. Our forms the thermally stable triplex with target dsRNA at acidic pH; and formation is accompanied remarkable light-up response of TO unit. The binding our to proceeds very rapidly, allowing real-time monitoring formation. Importantly, we found in functions universal pair opposite unit Furthermore, significantly more responsive...
Significance _ 0 \\\ K\\K\ -\\ + X0 -ao \\\\y }s\A8FNN NN N\ \\N D Figure 1 Conclusions for increasing values of theobserved difference (sample size and variance being equal).For given sample variance, large differences can reach significance; small (eventually) allow rejection the hypothesis that treatments differ by smallest relevant difference.In-between may be a region indifference (no conclusion possible).This process, mentioned Feinstein (1975) as calculation of'PB' applied to...
Biomarker for usefulness in diagnosing advanced fibrosis nonalcoholic fatty liver disease (NAFLD) is expected. In order to discover novel biomarkers NAFLD and its pathogenesis, we performed matabolomics screening. (1) The initial cohort was 44 patients. (2) This validation 105 patients, 26 primary biliary cirrhosis (PBC) 48 healthy controls. Using capillary electrophoresis liquid chromatography with mass spectrometry, analyzed low molecular weight metabolites these groups. 1. the cohort,...
A series of triplex-forming peptide nucleic acid (TFP) probes carrying a thiazole orange (TO) base surrogate through an alkyl linker was synthesized, and the interactions between these so-called tFIT purine-rich sequences within double-stranded RNA (dsRNA) were examined. We found that TO significantly affected both binding affinity fluorescence response upon triplex formation with target dsRNA. Among examined, connected propyl in increased by factor ten while maintaining its function as...
Red-emissive fluorescent probes have been developed by integration of quinoline blue or thiazole red as the base surrogate into triplex-forming PNAs, allowing selective sensing a sequence double-stranded RNA.
We revealed an association mechanism for PNA–dsRNA triplex formation based on a set of kinetic and thermodynamic data.
Peptide nucleic acid (PNA)-thiazole orange (TO) conjugates are developed as fluorescent probes capable of selective recognition 3'-overhanging nucleotides siRNAs for an accurate analysis the siRNA delivery process.
We report on the use of a peptide nucleic acid (PNA)-based fluorescent probe for analysis siRNA delivery to living cells. The probe, Py-AA-TO, possesses thiazole orange (TO) and pyrene moieties in C- N-termini PNA, can function as light-up capable selective binding 3′-overhanging nucleotides target siRNAs. affinitylabeling siRNAs with Py-AA-TO facilitates fluorescence imaging cellular uptake polymer-based carriers encapsulating (polyplexes) through endocytosis subsequent sequestration into...
We developed a new fluorescent peptide nucleic acid (PNA) probe, COT capable of simultaneous recognition 3′-overhang and double stranded sequences target small interfering RNA (siRNA).
Carrier-mediated delivery of small interfering RNAs (siRNAs) into the living cells is important for realization siRNA therapeutics that can silence target genes through RNA interference. We recently proposed a new strategy analyzing process based on affinity labeling with peptide nucleic acid (PNA)-based fluorescent probe (PyAATO; Py: pyrene, A: adenine; TO: thiazole orange) capable selectively binding to overhanging structures siRNAs. have prepared probes improved by conjugation cationic...
We report on a fluorescent pteridine derivative modified with guanidino group for the selective detection of an orphan guanine in RNA duplexes dissociation constant 31 ± 7 nM (pH 7.0, T = 20 °C). The significant effect attached to ring binding selectivity and affinity is discussed based examination thermodynamic parameters.
Electrical stimulation of the frog glossopharyngeal (GP) nerve evoked slow hyperpolarizing potentials (HPs) in taste cells. This study aimed to clarify whether HPs were postsynaptically induced The recorded intracellularly with a microelectrode. When Ca2+ concentration blood plasma was decreased approximately 0.5 mM, amplitude reduced and their latency lengthened. increased 20 shortened. Addition Cd2+ greatly lengthened latency. These data suggest that are dependent on presynaptic activities...
Abstract: We identified and characterized 125 I‐endothelin‐1 ( I‐ET‐1) binding sites in tumor capillaries isolated from human glioblastomas, using the quantitative receptor autoradiographic technique with pellet sections. Quantification was done computerized radioluminographic imaging plate system. High‐affinity ET receptors were localized glioblastomas surrounding brain tissues K D = 4.7 ± 1.0 × 10 −10 1.6 0.3 M , respectively; B max 161 38 140 37 fmol/mg, mean SEM, n 5). BQ‐123, a...
We report on a significant effect of locked nucleic acid (LNA) nucleobases the binding amiloride for abasic site (AP)-containing DNA duplexes. Fluorescence titration experiments showed that affinity target thymine (T) opposite an AP significantly improves duplexes possessing LNA flank site, compared to corresponding normal In particular, flanking both 5'- and 3'-sides are found be effective enhancement affinity. From thermodynamic characterization binding, loss in entropy is remarkably...