A5049465583- Cardiac electrophysiology and arrhythmias
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Cardiac Ischemia and Reperfusion
- RNA Interference and Gene Delivery
- Cannabis and Cannabinoid Research
- Estrogen and related hormone effects
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- Sleep and Wakefulness Research
- Organic Chemistry Cycloaddition Reactions
- Cardiac Arrest and Resuscitation
- Hormonal Regulation and Hypertension
- Neuroscience and Neuropharmacology Research
- Heart Failure Treatment and Management
- Cardiac, Anesthesia and Surgical Outcomes
Tohoku University
2007-2025
TOA EIYO (Japan)
2013-2022
Graduate School USA
2017
Sendai University
2017
Toho University
2015-2016
Red-emissive fluorescent probes have been developed by integration of quinoline blue or thiazole red as the base surrogate into triplex-forming PNAs, allowing selective sensing a sequence double-stranded RNA.
Abstract Objectives The inflammatory response plays an important role in the pathogenesis of many diseases central nervous system. Cannabinoids exhibit diverse pharmacological actions including anti-inflammatory activity. In this study, we tried to elucidate possible effects cannabinoids on lipopolysaccharide (LPS)-induced expression cytokine mRNAs rat cerebellar granule cells. Methods Inhibitory induction cells were determined by RT-PCR method. Key findings these cells, both mRNA and...
The inhibitory effects of sodium 3-guanidinocarbonyl-2-methyl-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine-9-ylmethyl sulfate monoethanolate (TY-51924) are selective for Na(+)/H(+) exchanger (NHE)-1 in PS120 cells expressing human NHE isoforms assayed by NH(4)Cl prepulse technique. median concentrations (micromolar) TY-51924 were 0.095 ± 0.008 (NHE-1), 0.621 0.093 (NHE-2), and >100 (NHE-3). In anesthetized dogs subjected to 90 minutes ischemia/300 reperfusion, intravenous bolus at 5 10 mg/kg...
Abstract Cinaciguat, a soluble guanylate cyclase ( sGC ) activator, was under clinical development for use in acute decompensated heart failure ADHF ), but discontinued due to occurrence of hypotension. We hypothesized that short‐term activation patients would exert vasodilative effect without hypotension irrespective disease state, using novel short‐acting TY ‐55002. The objective this study investigate the vasodilation and hemodynamic effects ‐55002 comparison with those cinaciguat....
Atrial enlargement is thought to provide arrhythmogenic substrates, leading the induction of atrial fibrillation (AF). In this study, we investigated anatomical, molecular biological, and electrophysiological characteristics remodeled atria in an animal model with chronic volume overload. We used rats that underwent abdominal aorto-venocaval shunt (AVS) surgery. vivo studies, marked changes electrocardiogram parameters, such as P-wave duration, PR interval, QRS width, well prolongation...
Electrical remodeling plays a pivotal role in maintaining the reentry during atrial fibrillation. In this study, we assessed influence of electrical on pharmacological manipulation refractoriness rabbits. We used an model rabbit, subjected to rapid pacing (RAP; 600 beats/min) for 2–4 weeks, leading shortening effective refractory period (AERP). Intravenous administration dl-sotalol (6 mg/kg), bepridil (1 amiodarone (10 mg/kg) or vernakalant (3 significantly prolonged AERP both control and...
【目的】ウサギ生体位心臓の催不整脈特性を明らかにするため，房室ブロックに伴う徐脈が心室不整脈の発生に及ぼす影響を房室ブロック急性期と慢性期で検討した．【方法】麻酔下のウサギにカテーテルアブレーションを用いて完全房室ブロック術を施した．房室ブロック急性期の検討（実験I）では心室を60回/分で駆動し，ニフェカラント（0.03, 0.3, 3mg/kg）を静脈内投与して心電図の変化を観察した．房室ブロック慢性期の検討（実験II）では，自発的な心室補充調律を確認した後にウサギを麻酔から回復させ，ホルター心電計を用いて不整脈の発生を記録した．【結果】実験Iで，ニフェカラントはQT間隔を延長させ，6例中5例に多形性心室頻拍（torsade de pointes : TdP）を出現させた．実験IIの検討では，23例中14例で術後に安定した補充調律が認められ，TdPが出現した．【結語】ウサギ心臓では，慢性期モデルと同様に，急性徐脈によりQT延長薬存在下で催不整脈特性が顕在化することが示された．
三重鎖形成ペプチド核酸（PNA）は，現在までに開発された合成分子のなかで，RNA二重鎖へ塩基配列選択的に結合し，かつDNA二重鎖よりも強く結合できる唯一の分子である．その特性を上手く活用することができれば，一本鎖RNA構造（領域）を標的とした従来の核酸プローブとは質的に異なる，全く新しい分析技術の創製につながると期待できる．そのためには，明瞭な蛍光シグナル機能を付与するとともに，三重鎖核酸形成のボトルネックである「塩基配列の制限」と「酸性条件」を克服する必要がある．本稿では，著者らが開発を進めたtriplex-forming forced intercalation of thiazole orange（tFIT）プローブについて報告する．