A5057745822- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Advanced Proteomics Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Metabolomics and Mass Spectrometry Studies
- Hydrogels: synthesis, properties, applications
- Lipid Membrane Structure and Behavior
- Polymer Surface Interaction Studies
- Microbial Community Ecology and Physiology
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- Advanced Biosensing Techniques and Applications
- Axon Guidance and Neuronal Signaling
- Surgical Simulation and Training
- Viral Infectious Diseases and Gene Expression in Insects
- Machine Learning in Materials Science
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Retinal Development and Disorders
- Computational Drug Discovery Methods
University of Cambridge
2018-2024
University College London
2013-2020
MRC Laboratory for Molecular Cell Biology
2013-2020
Medical Research Council
2015
Scuola Superiore Sant'Anna
2011
Laboratoire des systèmes et applications des technologies de l'information et de l'énergie
2009
Accurate animal cell division requires precise coordination of changes in the structure microtubule-based spindle and actin-based cortex. Here, we use a series perturbation experiments to dissect relative roles actin, cortical mechanics, shape formation. We find that, whereas actin cortex is largely dispensable for rounding timely mitotic progression isolated cells, it needed drive enable unperturbed morphogenesis under conditions confinement. Using different methods limit height, show that...
Significance Animal cells undergo a remarkable series of shape changes as they pass through mitosis and divide. In an epithelial tissue, the impact these morphogenetic processes depends strongly on orientation division. However, cues orienting divisions remain poorly understood. Here, we combine live imaging mechanical perturbations with computational modeling to investigate effects accompanying division in stretched monolayers absence neighbor exchange. We show that orient long cell axis...
Kymographs are graphical representations of spatial position over time, which often used in biology to visualise the motion fluorescent particles, molecules, vesicles, or organelles moving along a predictable path. Although kymographs tracks individual particles qualitatively easily distinguished, their automated quantitative analysis is much more challenging. exhibit low signal-to-noise-ratios (SNRs), and available tools that automate usually require manual supervision. Here we developed...
Proliferating animal cells are able to orient their mitotic spindles along interphase cell axis, setting up the axis of division, despite rounding as they enter mitosis. This has previously been attributed molecular memory and, more specifically, maintenance adhesions and retraction fibers in mitosis [1Théry M. Jiménez-Dalmaroni A. Racine V. Bornens Jülicher F. Experimental theoretical study spindle orientation.Nature. 2007; 447: 493-496Crossref PubMed Scopus (320) Google Scholar, 2Fink J....
The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type retina, are adapted to this environment still poorly understood. We compared proteomic data from cone- rod-rich retinae mice identified different expression profiles of rod-associated cells that converged on pathways representing extracellular matrix adhesion. In particular, epiplakin (EPPK1), which thought play role intermediate filament...
Most animal cells are surrounded by a cell membrane and an underlying actomyosin cortex. Both structures linked, they under tension. In-plane tension cortical both influence many cellular processes, including migration, division, endocytosis. However, while is regulated substrate stiffness, how responds to mechanical properties currently poorly understood. Here, we probed the effective of neurons fibroblasts cultured on glass polyacrylamide substrates varying stiffness using optical...
Minimally Invasive Surgery (MIS) is widespread in medical procedures aiming to provide incision-less surgery. Conventional MIS provides limited tissue manipulation, because of the constrained directionality force application and low number Degrees Freedom (DoFs). Robotic systems have been proposed overcome limitation this approach, but still require same incisions as traditional MIS. Thus, new approaches such minilaparoscopy, Single Incision Laparoscopic (SILS) Natural Orifice Transluminal...
Predicting drug properties is key in discovery to enable de-risking of assets before expensive clinical trials, and find highly active compounds faster. Interest from the Machine Learning community has led release a variety benchmark datasets proposed methods. However, it remains unclear for practitioners which method or approach most suitable, as different papers on methods, leading varying conclusions that are not easily compared. Our large-scale empirical study links together numerous...
Abstract During brain development, neurons extend axons to connect their target cells while initiating a maturation process, during which start expressing voltage-gated ion channels, form synapses, express synaptic transmitters and receptors, communicating via action potentials. Little is known about external factors regulating this process. Here, we identified environmental mechanics as an important regulator of neuronal maturation, molecular pathway linking tissue stiffness Using patch...
Abstract Kymographs are graphical representations of spatial position over time, which often used in biology to visualise the motion fluorescent particles, molecules, vesicles, or organelles moving along a predictable path. Although kymographs tracks individual particles qualitatively easily distinguished, their automated quantitative analysis is much more challenging. exhibit low signal-to-noise-ratios (SNRs), and available tools that automate usually require manual supervision. Here we...
Abstract Most animal cells are surrounded by a cell membrane and an underlying actomyosin cortex. Both structures linked, they under tension. In-plane tension cortical both influence many cellular processes, including migration, division, endocytosis. However, while is regulated substrate stiffness, how responds to mechanical properties currently poorly understood. Here, we probed the effective of neurons fibroblasts cultured on glass polyacrylamide substrates varying stiffness using optical...
Abstract Cortical stiffness is an important cellular property that changes during migration, adhesion, and growth. Previous atomic force microscopy (AFM) indentation measurements of cells cultured on deformable substrates suggested adapt their to surroundings. Here we show the applied by AFM onto results in a significant deformation underlying substrate if it softer than cells. This ‘soft effect’ leads underestimation cell’s elastic modulus when analyzing data using standard Hertz model, as...