A5088237407- Multiple Sclerosis Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Nerve injury and regeneration
- Peripheral Neuropathies and Disorders
- Mesenchymal stem cell research
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Myasthenia Gravis and Thymoma
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Immunology Research
- Eosinophilic Disorders and Syndromes
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Tumors and Oncological Cases
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Spinal Cord Injury Research
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
Vita-Salute San Raffaele University
2003-2024
Ca' Foncello Hospital
2024
University of Padua
2022-2024
Hôpital Nord
2024
Centre Hospitalier Universitaire Amiens-Picardie
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2019-2023
San Raffaele University of Rome
1994-2022
Neuroscience Institute
2014-2022
Istituti Clinici Scientifici Maugeri
2022
Recent evidence suggests that neural stem/precursor cells (NPCs) promote recovery in animal models with delayed neuronal death via a number of indirect bystander effects. A comprehensive knowledge how transplanted NPCs exert their therapeutic effects is still lacking. Here, we investigated the transplantation adult syngenic NPCs--injected intravenously 72 h after transient middle cerebral artery occlusion--on neurological recovery, histopathology and gene expression. NPC-transplanted mice...
Abstract Innovative pro-regenerative treatment strategies for progressive multiple sclerosis (PMS), combining neuroprotection and immunomodulation, represent an unmet need. Neural precursor cells (NPCs) transplanted in animal models of have shown preclinical efficacy by promoting remyelination releasing molecules sustaining trophic support neural plasticity. Here we present the results STEMS, a prospective, therapeutic exploratory, non-randomized, open-label, single-dose-finding phase 1...
Endogenous neural stem/precursor cells (NPCs) are considered a functional reservoir for promoting tissue homeostasis and repair after injury, therefore regenerative strategies that mobilize these have recently been proposed. Despite evidence of increased neurogenesis upon acute inflammatory insults (e.g. ischaemic stroke), the plasticity endogenous brain stem cell compartment in chronic CNS disorders remains poorly characterized. Here we show persistent inflammation, induced by immune...
Transplanted neural stem/precursor cells possess peculiar therapeutic plasticity and can simultaneously instruct several mechanisms in addition to cell replacement. Here, we interrogated the of after their focal implantation severely contused spinal cord. We injected syngeneic at proximal distal ends mouse cord analysed locomotor functions relevant secondary pathological events mice, fate transplanted cells, gene expression inflammatory infiltration injured site. used two different doses...
Transplanted neural stem/precursor cells (NPCs) display peculiar therapeutic plasticity in vivo. Although the replacement of was first expected as prime mechanism stem regenerative medicine, it is now clear that transplanted NPCs simultaneously instruct several mechanisms, among which might not necessarily prevail. A comprehensive understanding mechanism(s) by exert their lacking. This study designed a preclinical approach to test feasibility human NPC transplantation an outbreed nonhuman...
The systemic injection of neural stem/precursor cells (NPCs) provides remarkable amelioration the clinico-pathological features experimental autoimmune encephalomyelitis (EAE). This is dependent on capacity transplanted NPCs to engage concurrent mechanisms action within specific microenvironments in vivo. Among a wide range therapeutic actions alternative cell replacement, neuroprotective and immune modulatory capacities have been described. However, lacking detailed understanding by which...
Abstract The exclusive detrimental role of proinflammatory cytokines in demyelinating diseases the CNS, such as multiple sclerosis, is controversial. Here we show that intrathecal delivery an HSV-1-derived vector engineered with mouse IFN-γ gene leads to persistent (up 4 wk) CNS production and inhibits course a chronic-progressive form experimental autoimmune encephalomyelitis (EAE) induced C57BL/6 mice by myelin oligodendrocyte glycoprotein (MOG)35–55. Mice treated IFN-γ-containing before...
Eliciting the in situ accumulation and persistence patterns of stem cells following transplantation would provide critical insight toward human translation cell-based therapies. To this end, we have developed a strategy to track neural stem/precursor (NPCs) vivo using magnetic resonance (MR) imaging. Initially, evaluated three different human-grade superparamagnetic iron oxide particles for labeling NPCs found optimal be achieved with Resovist. Next, carried out experiments monitor...
Abstract Invariant NKT (inv. NKT) cells co‐express an invariant α β T cell receptor and the NK NK1.1 and, upon CD1d‐restricted recognition of glycosphingolipid antigen α‐galactosyl ceramide (αGalCer), secrete large amounts regulatory cytokines. We investigated whether αGalCer‐dependent activation inv. protects from experimental autoimmune encephalomyelitis (EAE), immune‐mediated disease central nervous system mimicking multiple sclerosis, induced in C57BL/6 mice by myelin oligodendrocyte...
IL-12 has been shown to be involved in the pathogenesis of Th1-mediated autoimmune diseases, but its role antibody-mediated pathologies is still unclear. We investigated effects exogenous and endogenous experimental myasthenia gravis (EAMG). EAMG an animal model for gravis, a T cell-dependent, autoantibody-mediated disorder neuromuscular transmission caused by antibodies muscle nicotinic acetylcholine receptor (AChR). Administration with Torpedo AChR (ToAChR) C57BL/6 (B6) mice resulted...
The functional significance of adult neural stem and progenitor cells in hippocampal-dependent learning memory has been well documented. Although the subventricular zone are known to migrate to, maintain reorganize olfactory bulb, it is less clear whether they functionally required for other processes. Using a conditional transgenic mouse model, selective ablation induced dramatic increase morbidity mortality central nervous system disorders characterized by excitotoxicity-induced cell death...
Myelin loss occurring in demyelinating diseases, including multiple sclerosis, is the leading cause of long-lasting neurological disability adults. While endogenous remyelination, driven by resident oligodendrocyte precursor cells (OPCs), might partially compensate myelin early phases disorders, this spontaneous reparative potential fails at later stages. To investigate cellular mechanisms sustaining remyelination we focused our attention on neural (eNPCs) located within subventricular zone...
Background There are two generally accepted strategies for treating multiple sclerosis (MS), preventing central nervous system (CNS) damage indirectly through immunomodulatory interventions and/or repairing CNS by promoting remyelination. Both approaches also provide neuroprotection since they can prevent, or directly, axonal damage. Objective Recent experimental and clinical evidence indicates that the novel drug laquinimod exert a neuroprotective role in MS. Whether laquinimod-mediated is...
In multiple sclerosis, the pathological interaction between autoreactive Th cells and mononuclear phagocytes in CNS drives initiation maintenance of chronic neuroinflammation. Here, we found that intrathecal transplantation neural stem/precursor (NPCs) mice with experimental autoimmune encephalomyelitis (EAE) impairs accumulation inflammatory monocyte-derived (MCs) CNS, leading to improved clinical outcome. Secretion IL-23, IL-1, TNF-α, cytokines required for terminal differentiation cells,...
The role of central nervous system (CNS) glia in sustaining self-autonomous inflammation and driving clinical progression multiple sclerosis (MS) is gaining scientific interest. We applied a single transcription factor (SOX10)-based protocol to accelerate oligodendrocyte differentiation from human induced pluripotent stem cell (hiPSC)-derived neural precursor cells, generating self-organizing forebrain organoids. These organoids include neurons, astrocytes, oligodendroglia, hiPSC-derived...
Human herpesvirus 6 (HHV-6) and HHV-7 are closely related DNA viruses, sharing biologic antigenic properties an overlapping genome organization.1,2 Primary infections with HHV-6 and, in some cases, cause exanthem subitum or fever, without rash.1,2 Major pathologies include reactivation immunocompromised individuals. An association between active infection MS was proposed based on detection of viral antigen oligodendrocytes surrounding plaques,3 sequences sera,4 circulating anti-HHV-6...
The role of central nervous system (CNS) glia in sustaining self-autonomous inflammation and driving clinical progression multiple sclerosis (MS) is gaining scientific interest. We applied a single transcription factor (