A5016550571- Multiple Sclerosis Research Studies
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Functional Brain Connectivity Studies
- Advanced Neuroimaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Medical Imaging Techniques and Applications
- Blood Pressure and Hypertension Studies
- Cardiac Imaging and Diagnostics
- Renin-Angiotensin System Studies
- Barrier Structure and Function Studies
- Advanced Radiotherapy Techniques
- RNA regulation and disease
- Nitric Oxide and Endothelin Effects
- Chemotherapy-induced cardiotoxicity and mitigation
- Extracellular vesicles in disease
- Glioma Diagnosis and Treatment
- Electromagnetic Fields and Biological Effects
- Cerebrovascular and Carotid Artery Diseases
- Acute Myocardial Infarction Research
- Diet and metabolism studies
University Medical Center Utrecht
2013-2024
Utrecht University
1998-2023
Heidelberg University
2002-2022
University Hospital Heidelberg
2002-2022
Weatherford College
2020
Genmab (Netherlands)
2011
Biomedical Primate Research Centre
2010-2011
Erasmus MC
2011
University of Vienna
2011
University Medical Center Groningen
2011
Non-human primate neuroimaging is a rapidly growing area of research that promises to transform and scale translational cross-species comparative neuroscience. Unfortunately, the technological methodological advances past two decades have outpaced accrual data, which particularly challenging given relatively few centers necessary facilities capabilities. The PRIMatE Data Exchange (PRIME-DE) addresses this challenge by aggregating independently acquired non-human magnetic resonance imaging...
Enhanced cerebrovascular permeability and cellular infiltration mark the onset of early multiple sclerosis lesions. So far, precise sequence these events their role in lesion formation disease progression remain unknown. Here we provide quantitative evidence that blood–brain barrier leakage is an event precedes massive development acute experimental allergic encephalomyelitis (EAE), animal correlate sclerosis. Cerebrovascular monocytes infiltrates were separately monitored by vivo MRI during...
Transplanted neural stem/precursor cells (NPCs) display peculiar therapeutic plasticity in vivo. Although the replacement of was first expected as prime mechanism stem regenerative medicine, it is now clear that transplanted NPCs simultaneously instruct several mechanisms, among which might not necessarily prevail. A comprehensive understanding mechanism(s) by exert their lacking. This study designed a preclinical approach to test feasibility human NPC transplantation an outbreed nonhuman...
<h3>Background</h3> Diffuse abnormalities in the white matter (WM), ie, so-called diffusely abnormal WM (DAWM), as observed on magnetic resonance imaging (MRI), may contribute to development of clinical disability multiple sclerosis (MS). Underlying pathologic and MRI characteristics DAWM are largely unknown. <h3>Objectives</h3> To explore describe histopathologic radiologic chronic MS. <h3>Design</h3> An postmortem correlative study. <h3>Methods</h3> We analyzed 17 formalin-fixed...
Purpose: MR-guided Radiation Therapy (MRgRT) allows for high-precision radiotherapy under real-time MR visualization. This enables margin reduction and subsequent dose escalation which may lead to higher tumor control less toxicity. The Unity MR-linac (Elekta AB, Stockholm, Sweden) integrates a linear accelerator with 1.5T diagnostic quality MRI an online adaptive workflow. A prospective international registry was established facilitate the evidence-based implementation of into clinical...
PURPOSE: To correlate quantitative magnetic resonance (MR) imaging data (ie, relaxation times and magnetization transfer ratios [MTRs]) with histopathologic findings of demyelination axonal disease in cervical spinal cord specimens from patients multiple sclerosis (MS) control subjects. MATERIALS AND METHODS: Formaldehyde-fixed 11 MS—three men eight women (mean age at death, 66 years ± 11.3 [standard deviation])—and two female subjects without neurologic (83 41 death) were examined 4.7 T....
The experimental autoimmune encephalomyelitis (EAE) model in the common marmoset approximates recognized features of human disease multiple sclerosis (MS) with regard to its clinical presentation as well neuropathological and radiological aspects lesions brain spinal cord. IL-12 is a proinflammatory cytokine that produced by APC promotes differentiation Th1 effector cells. developing patients MS EAE-affected animals. Previously it was shown interference pathways effectively prevents EAE...
To label human monocytes with superparamagnetic iron oxide (SPIO) and compare labeling efficiency that of ultrasmall SPIO (USPIO) evaluate the effect incorporation on cell viability, migratory capacity, proinflammatory cytokine production.The study was approved by institutional ethics committee; informed consent obtained from donors. Freshly isolated were labeled particles two sizes, USPIOs 30 nm SPIOs 150 nm, for 1.5 hours in culture medium containing 0.1, 0.5, 1.0, 3.7 mg per milliliter....
Abstract The immune system is characterized by the preferential migration of lymphocytes through specific tissues (i.e., tissue tropism). Tissue tropism mediated, in part, α4 integrins expressed T lymphocytes. α4β1 integrin mediates memory into CNS, whereas α4β7 preferentially gastrointestinal tissue. This paradigm was established primarily from investigations rodents; thus, objective this investigation to determine if blocking exclusively would affect CNS primates. effects dual and...
<h3>Importance</h3> Cardiovascular disease (CVD) is common in patients treated for breast cancer, especially with systemic treatment and radiotherapy those preexisting CVD risk factors. Coronary artery calcium (CAC), a strong independent factor, can be automatically quantified on planning computed tomography (CT) scans may help identify at increased risk. <h3>Objective</h3> To evaluate the association of CAC coronary (CAD) cancer. <h3>Design, Setting, Participants</h3> In this multicenter...
Abstract Inhibition of CD40-CD40 ligand interaction is a potentially effective approach for treatment autoimmune diseases, such as multiple sclerosis. We have investigated this concept with chimeric antagonist anti-human CD40 mAb (ch5D12) in the marmoset monkey experimental encephalomyelitis (EAE) model. Marmosets were immunized recombinant human myelin oligodendrocyte glycoprotein (rMOG) and treated from day before immunization (day −1) until 50 either ch5D12 (5 mg/kg every 2–4 days) or...
Experimental autoimmune encephalomyelitis in the neotropical primate common marmoset (Callithrix jacchus) is a relevant animal model of multiple sclerosis. T cells specific for peptide 34 to 56 myelin/oligodendrocyte glycoprotein (MOG34-56) have central pathogenic role this model. The aim study was assess requirement innate immune stimulation activation core mechanism. Marmoset monkeys were sensitized against synthetic MOG34-56 alone or combination with nonencephalitogenic MOG74-96...
In 2018, the first online adaptive magnetic resonance (MR)-guided radiotherapy (MRgRT) system using a 1.5-T MR-equipped linear accelerator (1.5-T MR-Linac) was clinically introduced. This enables radiotherapy, in which radiation plan is adapted to size and shape changes of targets at each treatment session based on daily MR-visualized anatomy.
IL-12p40 is a shared subunit of two cytokines with overlapping activities in the induction autoreactive Th1 cells and therefore potential target therapy Th1-mediated diseases. We have examined whether ongoing disease nonhuman primate model multiple sclerosis (MS) can be suppressed new human IgG1kappa Ab against IL-12p40. Lesions developing brain white matter were visualized characterized standard magnetic resonance imaging techniques. To reflect treatment MS patients, was initiated after...
Magnetic resonance imaging (MRI) has been applied to visualize monocyte infiltration with the use of intravenously injected ultrasmall superparamagnetic iron oxide (USPIO). However, USPIO uptake in vivo remains elusive, and heterogeneous enhancement patterns observed by MRI point multiple pathophysiological events. This study focused on specific into brain transfusion (SPIO)-labeled monocytes a rat model neuroinflammation, experimentally induced photothrombosis (PT). At day 5 after lesion...
Abstract Experimental autoimmune encephalomyelitis (EAE) induced with recombinant human myelin/oligodendrocyte glycoprotein in the common marmoset is a useful preclinical model of multiple sclerosis which white matter lesions can be well visualized MRI. In this study we characterized lesion progression quantitative vivo MRI (4.7 T; T 1 relaxation time ± Gd‐DTPA; 2 time; magnetization transfer ratio, MTR, imaging) and correlated end stage presentation ex (formaldehyde fixed brains; times;...
Abstract The recombinant human (rh) myelin/oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) model in the common marmoset is characterized by 100% disease incidence, a chronic course, and variable time interval between immunization neurological impairment. We investigated whether monkeys with fast slow progression display different anti-MOG T or B cell responses analyzed underlying pathogenic mechanism(s). results show that progressor significantly...
Small-animal single photon emission computed tomography (SPECT) with focused multi-pinhole collimation geometries allows scanning modes in which large amounts of photons can be collected from specific volumes interest. Here we present new tools that improve targeted imaging organs and tumours, validate the effects improved targeting pinhole focus.A SPECT system 75 pinholes stationary detectors was used (U-SPECT-II). An XYZ stage automatically translates animal bed a sequence order to scan...
Induction of experimental autoimmune encephalomyelitis (EAE) has been documented in common marmosets using peptide 34-56 from human myelin/oligodendrocyte glycoprotein (MOG(34-56) ) incomplete Freund's adjuvant (IFA). Here, we report that this EAE model is associated with widespread demyelination grey and white matter. We performed an in-depth analysis the specificity, MHC restriction functions activated T cells model, which likely cause autoantibody-independent manner. T-cell lines isolated...