Herein, we describe a new family of tris chelate homoleptic Ru (II) complexes, [Ru(N^N) 3 ] 2+ , where the role diimine‐type ligands (N^N) was fulfilled by 2‐pyridyl (PTZ) or 2‐quinolyl tetrazole (QTZ) derivatives decorated with various alkyl substituents at N‐2 position ring. The complexes general formula [Ru (PTZ‐R) and (QTZ‐R) were obtained as mixtures facial ( fac ) meridional mer isomers, suggested NMR 1 H, 13 C) experiments, confirmed in case ‐[Ru (QTZ‐Me) X‐ray crystallography....

Human herpesvirus 6 (HHV-6) and HHV-7 are closely related DNA viruses, sharing biologic antigenic properties an overlapping genome organization.1,2 Primary infections with HHV-6 and, in some cases, cause exanthem subitum or fever, without rash.1,2 Major pathologies include reactivation immunocompromised individuals. An association between active infection MS was proposed based on detection of viral antigen oligodendrocytes surrounding plaques,3 sequences sera,4 circulating anti-HHV-6...

The full-length cDNA of the murine homolog human nectin1δ (mNectin1δ), also known as poliovirus receptor related 1 (PRR1) or herpesvirus entry mediator C, was cloned and showed a >90% identity with its counterpart. mNectin1δ is expressed in some cell lines, exemplified by NIH 3T3 L cells, tissues. It mediates an extended range herpes simplex virus (HSV) strains, porcine pseudorabies (PrV), bovine 1. A soluble form blocked infectivity (hNectin1δ)-expressing suggesting physical interaction...

A coupled enzyme assay able to monitor the kinetics of reactions catalyzed by phosphate- or pyrophosphate-releasing enzymes is presented here. The based on concerted action inorganic pyrophosphatase (PPase), purine nucleoside phosphorylase (PNPase), and xanthine oxidase (XOD). In presence phosphate, PNPase catalyzes phosphorolysis inosine, generating hypoxanthine, which oxidized uric acid XOD. accordingly formed can be spectrophotometrically monitored at 293 nm, taking advantage a molar...

A family of antigenically related proteins present in cells infected with human herpesvirus 7 (HHV-7), designated phosphoprotein 85 (pp85), comprises a complex proteins, which the 85-kDa species is phosphorylated. pp85 major determinant response to HHV-7 infection (L. Foà-Tomasi, E. Avitabile, L. Ke, and G. Campadelli-Fiume, J. Gen. Virol. 75:2719-2727, 1994; M. P. Fiorilli, 77:511-518, 1996; B. Black et al., Clin. Diagn. Lab. Immunol. 3:79-83, 1996). By immunoscreening cDNA library from...

The design, preparation and characterization of four new Re(<sc>i</sc>) tetrazolato complexes together with the study their luminescent sensing abilities toward Zn(<sc>ii</sc>), Cd(<sc>ii</sc>) Cu(<sc>ii</sc>) are described herein.

The assembly from modular parts is an efficient approach for creating new devices in Synthetic Biology. In the "bottom-up" designing strategy, are characterized advance, and then mathematical modeling used to predict outcome of final device. A prerequisite bottom-up design that biological behave a way when assembled together. We designed synthetic device post-transcriptional regulation gene expression tested if could be described features its components. Modular showed unpredictable behavior...

Two neutral cyclometalated Ir(iii)-tetrazolato complexes that differ by variations of the substituents on either phenylpyridine or tetrazolate ligand have been converted into corresponding methylated and cationic analogues. NMR (1H 13C) characterization Ir(iii) provided results in agreement with chemo- regioselective character methylation at N-3 position Ir(iii)-coordinated tetrazolato ring. This evidence was further corroborated analysis molecular structures obtained X-ray diffraction. In...

This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available enzyme-linked fluorescent assay (ELFA) VIDAS ECOLI O157 for detecting Escherichia coli in mincemeat. In addition, a PCR-based survey on Shiga-toxin-producing E. (STEC) mincemeat collected Italy is presented. Real-time PCR assays targeting stx genes and specific STEC sequence (SILO157, small inserted locus of O157) were tested their sensitivity spiked samples. After overnight enrichment,...

Directed evolution of Escherichia coli β‐galactosidase into variants featuring β‐glucosidase activity was challenged. To this end, mutagenesis lacZ performed by replication in E. CC954, a mutator strain containing DNA polymerase III defective 3′→5′ exonuclease activity. β‐Galactosidase can be isolated upon hosted the self‐transmissible episome F′128. Optimal achieved propagation CC954/F′128 cultures for 15 generations; further growth 37 or 55 generations imposes high mutational load on and...

Within the general framework of our past and current studies dealing with investigation photophysical properties biological behavior family tetrazolato tetrazole Re(i) complexes, we have endeavored to investigate their potential in luminescent staining proteins purified by acrylamide gel electrophoresis. With aim provide first examples complexes be exploited for this specific purpose, designed prepared four new Re(i)-based species formula fac-[Re(CO)3(N^N)(Tph)]2-/0, where Tph is...

Ten manganese(I) tricarbonyl diimine complexes bound to variably functionalised 5‐aryl‐tetrazolato ligands were prepared, and their photochemical properties investigated. Upon exposure light at 365 nm, each complex decomposed its free tetrazolato ligands, simultaneously dissociating three CO as evidenced by changes in the IR spectra of irradiated over time. The anti‐bacterial one these tested against Escherichia coli . While displayed no effect on bacterial growth dark, pre‐irradiated...

The conversion of industrial by-products into high-value added compounds is a challenging issue. Crude glycerol, by-product the biodiesel production chain, could represent an alternative carbon source for cultivation oleaginous yeasts. Here, we developed five minimal synthetic glycerol-based media, with different C/N ratios, and analyzed biomass fatty acids by Yarrowia lipolytica Po1g strain. We identified two media at expense which Y. was able to accumulate ∼5 g L(-1) 0.8 (0.16 per dry...

Abstract Extremely stable water‐in‐oil macroemulsions have been obtained by dispersing water in isooctane the presence of lecithin. Either prokaryotic ( Escherichia coli ) and eukaryotic Saccharomyces cerevisiae Rhodotorula minuta cells hosted these are viable for weeks despite consistent excess organic solvent (ranging from 70 to 84%, v/v) ternary systems. Conjugation occurs upon mixing containing F + or − strains, indicating mass transfer between droplets. Populations yeasts macroemulsion...

The Mycobacterium tuberculosis low-molecular weight protein tyrosine phosphatase (MptpA) is responsible for the inhibition of phagosome-lysosome fusion and essential bacterium pathogenicity. This implies that M. not exposed to a strongly acidic environment in vivo, enabling successful propagation host cells. Remarkably, MptpA has been previously structurally functionally investigated, with special emphasis devoted enzyme properties at pH 8.0. Considering virulence strictly dependent on...

ABSTRACT Human antibodies raised in response to human herpesvirus 7 (HHV-7) infection are directed predominantly one or more HHV-7-infected cell proteins with apparent molecular masses of about 85 89 kDa. The genes that encode these unknown. However, several HHH-7 possibly this mass range have been identified. Thus, the encoded by open reading frame U14 (85 kDa) and U11 (86 were expressed as recombinant bacteria. Of 13 serum specimens recognized 85- 89-kDa protein(s) cells immunoblotting, 12...

Chromosome replication in Escherichia coli is accomplished by the multimeric enzyme DNA polymerase III; relevance, vivo, of ϵ subunit (encoded dnaQ ) for processivity and fidelity III has been evaluated. To this aim, conditionally silenced means vivo expression different antisense RNAs. Unexpectedly, presence Shine–Dalgarno sequence essential effectiveness constructs. Silencing induces a severe decrease growth rate not paralleled high mutation frequencies, suggesting that primarily affects III.

Antisense RNA molecules are powerful tools for controlling the expression of specific genes but their use in prokaryotes has been limited by unpredictable antisense effectiveness. Moreover, appreciation molecular mechanisms associated with silencing bacteria is still restricted. Here we report our attempts to define an effective strategy E. coli, and dissect observed process. constructs complementary different regions lacZ were investigated, was exclusively upon hybridising 5'UTR lac...