The effect of various concentrations aminoethoxyvinylglycine (AVG; 0.32 and 1.28 mM), an ethylene biosynthesis inhibitor, the polyamines putrescine (10 spermidine (0.1, 1 5 mM) spermine (2 on peach (Prunus persica L. Batsch cv. Redhaven) fruit ripening was evaluated under field conditions. Treatments were performed 19 (polyamines) 8 (AVG) days before harvest. Fruit growth (diameter, fresh dry weight), flesh firmness, soluble solids content emission determined treated untreated (controls)...

The effect of methyl jasmonate (MJ) on de novo shoot formation and polyamine metabolism was investigated in thin layer explants tobacco (Nicotiana tabacum L. cv. Samsun). A relatively low concentration MJ (0.1 microM) enhanced explant fresh weight, but had no the final number shoots per while higher concentrations (1 10 significantly inhibited organogenesis. histological study revealed that, with increasing MJ, meristemoids domes declined incidence cell hypertrophy increased. In cultured...

S-Adenosyl-L-methionine decarboxylase (AdoMetDC; EC 4.1.1.50) is one of the key regulatory enzymes in biosynthesis polyamines. Isolation genomic and cDNA sequences from rice Arabidopsis had indicated that this enzyme encoded by a small multigene family monocot dicot plants. Analysis rice, maize AdoMetDC species revealed possesses an extended C-terminus relative to human enzymes. Interestingly, we discovered all expressed plant mRNA 5´ leader contain highly conserved pair overlapping upstream...

Journal Article De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis Get access M. ALTAMURA, ALTAMURA 1Dipartimento di Biologia Vegetale, Università La SapienzaItaly Search for other works this author on: Oxford Academic PubMed Google Scholar P. TORRIGIANI, TORRIGIANI 2Dipartimento evoluziomstica sperimentale, BolognaRoma, Italy, F. CAPITANI, CAPITANI S. SCARAMAGLI, SCARAMAGLI N. BAGNI Experimental Botany, Volume 42, Issue 12, December 1991, Pages...

S-Adenosyl-L-methionine decarboxylase (AdoMetDC; EC 4.1.1.50) is one of the key regulatory enzymes in biosynthesis polyamines. Isolation genomic and cDNA sequences from rice Arabidopsis had indicated that this enzyme encoded by a small multigene family monocot dicot plants. Analysis rice, maize AdoMetDC species revealed possesses an extended C-terminus relative to human enzymes. Interestingly, we discovered all expressed plant mRNA 5´ leader contain highly conserved pair overlapping upstream...

• Change is reported in the biosynthetic and oxidative activity of hypersensitive (NN) susceptible (nn) tobacco (Nicotiana tabacum) plants response to mosaic virus (TMV). Mature leaves nn NN were collected over 0–72 h as uninoculated controls or after inoculation with TMV phosphate buffer (mock-inoculation). The polyamine was analysed by measuring gene expression S-adenosylmethionine decarboxylase (SAMDC), arginine-(ADC) ornithine decarboxylases (ODC); incorporation labelled putrescine;...

Pasta, the Italian product par excellence, is made of pure durum wheat. The use Triticum derived semolina in fact mandatory for pasta, which aestivum species considered a contamination that must not exceed 3% maximum level. Over last 50 years, various electrophoretic, chemical, and immuno-chemical methods have been proposed aimed to track possible presence common wheat pasta. More recently, new generation methods, based on DNA (DeoxyriboNucleic Acid ) analysis, has developed this aim....

The effects of two inhibitors polyamine (spermidine and spermine) biosynthesis, cyclohexylamine (CHA; 5 10 m M ) methylglyoxal(bis‐guanylhydrazone) (MGBG; 0.1, 0.5 1 ), on the organogenic response in vegetative bud‐forming tobacco ( Nicotiana tabacum L. cv. Samsun) thin layer explants were evaluated micro‐ macroscopically at different times during culture. final number buds formed percentage was significantly reduced by both inhibitors, but much more so MGBG than CHA. This inhibitory effect...

Previous results indicated that in shoot‐forming tobacco thin layers the putative inhibitor of S‐adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.21) activity methylglyoxal(bis‐guanylhydrazone) (MGBG) inhibited meristemoid/primordia formation while enhancing conjugated polyamine accumulation and ethylene biosynthesis, thus showing an inverse relationship between two phenomena ( Scaramagli et al. 1999). In order to better understand how MGBG causes overproduction by using same model system,...

This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available enzyme-linked fluorescent assay (ELFA) VIDAS ECOLI O157 for detecting Escherichia coli in mincemeat. In addition, a PCR-based survey on Shiga-toxin-producing E. (STEC) mincemeat collected Italy is presented. Real-time PCR assays targeting stx genes and specific STEC sequence (SILO157, small inserted locus of O157) were tested their sensitivity spiked samples. After overnight enrichment,...

Abstract The regulation of S‐adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.1), a key enzyme polyamine biosynthesis, was analysed at the level transcript accumulation, activity, and free conjugated accumulation during floral development in tobacco (Nicotiana tabacum L., cv. Samsun). SAMDC investigated five different stages flower each whorl. Using PCR‐derived homologous probe, approximately 2.0 kb detected. In whorls, lower anthers than ovaries. latter, activity did not parallel...

Radioactivity translocation after [ 14 C]‐spermidine application over the third trifoliate leaf of soybean plants ( Glycine max . [L.] Merr, cv. Williams) was checked during first 72 h short day (SD) treatment to study involvement polyamines (PAs) in photoperiodic flowering induction. PAs and/or their metabolites were translocated from supplied all parts plant. reached its highest concentration upper portion stem, i.e. apical bud and youngest leaf. After beginning inductive night, detected...