A5016278352- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Systemic Sclerosis and Related Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Inflammasome and immune disorders
- COVID-19 Clinical Research Studies
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood disorders and treatments
- Atherosclerosis and Cardiovascular Diseases
- Biomarkers in Disease Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood Coagulation and Thrombosis Mechanisms
- Vasculitis and related conditions
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Long-Term Effects of COVID-19
- Nitric Oxide and Endothelin Effects
- Adipokines, Inflammation, and Metabolic Diseases
- S100 Proteins and Annexins
- Extracellular vesicles in disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Autophagy in Disease and Therapy
- Mast cells and histamine
- Eicosanoids and Hypertension Pharmacology
Vita-Salute San Raffaele University
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2018-2024
IRCCS Ospedale San Raffaele
2007-2019
San Raffaele University of Rome
2017
Pontifícia Universidade Católica de São Paulo
2008
Catholic University of America
2006-2007
Università Cattolica del Sacro Cuore
2005
Consejo Nacional de Investigaciones Científicas y Técnicas
1989-2001
New York Academy of Medicine
2001
Platelet microparticles from patients with systemic sclerosis prompt endothelial cell activation, neutrophil recruitment, and lung fibrosis.
Human type 1 diabetes (T1D) is an autoimmune disease associated with major histocompatibility complex polymorphisms, β-cell autoantibodies, and autoreactive T cells. However, there increasing evidence that innate cells may also play critical roles in T1D. We aimed to monitor peripheral immune early stages of T1D (i.e., healthy autoantibody-positive subjects) more advanced phases the at onset years after diagnosis). found a mild but significant reproducible neutropenia both precedes...
Abstract Background Host inflammation contributes to determine whether SARS-CoV-2 infection causes mild or life-threatening disease. Tools are needed for early risk assessment. Methods We studied in 111 COVID-19 patients prospectively followed at a single reference Hospital fifty-three potential biomarkers including alarmins, cytokines, adipocytokines and growth factors, humoral innate immune neuroendocrine molecules regulators of iron metabolism. Biomarkers hospital admission together with...
The link between platelet activation and vascular injury in Systemic Sclerosis (SSc) is poorly characterized. Here we report that results i) the translocation from cytoplasm to surface of HMGB1, a prototypical DAMP signal associated with tissue regeneration ii) release derived microparticles (PDμP) expressing HMGB1. Decreased HMGB1 content (334.6 ± 21.2 vs 587.1 11.1 AUF, P < 0.001) outer leaflet plasma membrane (17.8 3.5 4.5 0.5%, characterize circulating platelets SSc patients (n = 29)...
Abstract Objective To assess local expression and plasma levels of pentraxin 3 (PTX3) in patients with giant cell arteritis (GCA). Methods Plasma serum samples were obtained from 75 GCA (20 whom had experienced optic nerve ischemia the previous weeks 24 symptom onset 6 months no history ischemia) 63 controls (35 age‐matched healthy subjects, 15 rheumatoid arthritis, 13 chronic stable angina). In 9 was recently diagnosed, circulating interleukin‐1β (IL‐1β), IL‐2, IL‐4, IL‐6, IL‐7, IL‐8,...
An abnormal generation of reactive oxygen species (ROS) is thought to contribute systemic sclerosis (SSc), fostering autoimmunity, fibrosis, and vascular inflammation. The function the prototypic damage-associated molecular pattern, high mobility group box 1 (HMGB1), depends on its redox status. Here we investigate whether oxidative stress regulates cross-talk between leukocytes platelets via HMGB1, thus contributing vessel inflammation in SSc.The oxidation HMGB1 amplified ability activate...
Abstract Pentraxin 3 (PTX3) plays cardioprotective and anti-atherogenic roles in murine models. PTX3 blood levels raise during early acute myocardial infarction (AMI). Neutrophils from healthy subjects physiologically contain secondary (also called specific) granules. In this study, we report that circulating neutrophils release preformed the phase of AMI (within 6 h onset clinical symptoms). Depletion intracellular correlates with increased plasma platelet–neutrophil heterotypic aggregates....
<h3>Background</h3> Cardiovascular disease is important in rheumatoid arthritis (RA). Tissue factor (TF) expressed upon platelet activation and initiates coagulation. Anti-tumour necrosis factor-α (TNFα) agents seem to decrease RA-associated cardiovascular events. We investigated whether (1) TNFα activates human platelets (2) pharmacological blockade modulates the platelet-leucocyte reciprocal RA. <h3>Design</h3> The expression of receptors has been assessed by flow cytometry immunogold...
Summary In PMN/platelet suspensions stimulated by fMLP giant mixed aggregates are formed and TxB2 LTC4 synthesized as the result of cooperation in arachidonic acid (AA) metabolism during cell/cell contact. PMN-derived cathepsin G induced expression P-selectin on platelet surface. GE12, an antibody against P-selectin, significantly reduced cell aggregates. GE12 did not affect aggregation supernatants, indicating that inhibitory effect depends inhibition adhesion. production fMLP. As...
Background Neutrophils are involved in thrombus formation. We investigated whether specific features of neutrophil activation characterize patients with acute coronary syndromes (ACS) compared to stable angina and systemic inflammatory diseases. Methods Findings The myeloperoxidase (MPO) content circulating neutrophils was determined by flow cytometry 330 subjects: 69 consecutive (ACS), chronic (CSA), 50 inflammation due either non-infectious (acute bone fracture), infectious (sepsis) or...
Abstract Neutrophil extracellular traps (NETs) are DNAs products involved in immune process. Obesity through a low-grade chronic inflammation determines neutrophil activation, but it is still unclear its role NETs formation. Here we analyzed the levels healthy and morbid obese, their association with anthropometric glyco-metabolic parameters changes after bariatric surgery. For this study, enrolled 73 patients obesity (BMI ≥40 kg/m 2 or ≥35 + comorbidity) eligible to sleeve gastrectomy. In...
The role of neutrophils in systemic sclerosis (SSc) remains incompletely understood. To address this, blood samples from 39 SSc patients, healthy controls, and 22 lupus erythematosus (SLE) patients were analyzed. In SSc, exhibited substantial activation, evidenced by granule mobilization, elevated plasma levels Neutrophil Extracellular Trap (NET) byproducts, upregulated TIE2 expression. parallel, they underwent metabolic reprogramming, characterized increased autophagy, likely to support the...