A5024476656- Mycobacterium research and diagnosis
- Cystic Fibrosis Research Advances
- Tuberculosis Research and Epidemiology
- Gut microbiota and health
- COVID-19 Clinical Research Studies
- Bacterial biofilms and quorum sensing
- Infectious Diseases and Mycology
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Long-Term Effects of COVID-19
- Pediatric health and respiratory diseases
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Inhalation and Respiratory Drug Delivery
- Vibrio bacteria research studies
- Tracheal and airway disorders
- Antimicrobial Resistance in Staphylococcus
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Pneumonia and Respiratory Infections
- Calcium signaling and nucleotide metabolism
- Psoriasis: Treatment and Pathogenesis
- Antimicrobial Peptides and Activities
IRCCS Ospedale San Raffaele
2015-2024
Vita-Salute San Raffaele University
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015-2024
San Raffaele University of Rome
2022
Hospital Universitari i Politècnic La Fe
2018
University of Liverpool
2015
The opportunistic pathogen Pseudomonas aeruginosa is able to thrive in diverse ecological niches and cause serious human infection. P. environmental strains are producing various virulence factors that required for establishing acute infections several host organisms; however, the phenotypic variants favour long-term persistence cystic fibrosis (CF) airways. Whether strains, which have adapted CF-niche, lost their competitive fitness other environment remains be investigated. In this paper,...
Patients infected with SARS-CoV-2 range from asymptomatic, to mild, moderate or severe disease evolution including fatal outcome. Thus, early predictors of clinical outcome are highly needed. We investigated markers neural tissue damage as a possible sign multisystem involvement assess their prognostic value on survival transfer intensive care unit (ICU). collected blood 104 patients the day admission emergency room and measured neurofilament light chair (NfL), glial fibrillary acidic...
Abstract Background Host inflammation contributes to determine whether SARS-CoV-2 infection causes mild or life-threatening disease. Tools are needed for early risk assessment. Methods We studied in 111 COVID-19 patients prospectively followed at a single reference Hospital fifty-three potential biomarkers including alarmins, cytokines, adipocytokines and growth factors, humoral innate immune neuroendocrine molecules regulators of iron metabolism. Biomarkers hospital admission together with...
Pseudomonas aeruginosa can establish life-long airways chronic infection in patients with cystic fibrosis (CF) pathogenic variants distinguished from initially acquired strain. Here, we analysed chemical and biological activity of P. Pathogen-Associated Molecular Patterns (PAMPs) clonal strains, including mucoid non-mucoid phenotypes, isolated during a period up to 7.5 years CF patient. Chemical structure by MS spectrometry defined lipopolysaccharide (LPS) lipid A peptidoglycan (PGN)...
The Gram-negative bacteria Pseudomonas aeruginosa and Burkholderia cenocepacia are opportunistic human pathogens that responsible for severe nosocomial infections in immunocompromised patients those suffering from cystic fibrosis (CF). These two have been shown to form biofilms the airways of CF make such more difficult treat. Only recently scientists begun appreciate complicated interplay between microorganisms during polymicrobial infection airway implications they may disease prognosis...
Staphylococcus aureus thymidine-dependent small-colony variants (TD-SCVs) are frequently isolated from patients with chronic S. infections after long-term treatment trimethoprim-sulfamethoxazole (TMP-SMX). While it has been shown that TD-SCVs were associated mutations in thymidylate synthase (TS; thyA), the impact of such on protein function is lacking. In this study, we showed thyA leading to inactivity TS proteins, and led tremendous physiology virulence. Whole DNA microarray analysis...
Abstract Repeated cycles of infections, caused mainly by Pseudomonas aeruginosa, combined with a robust host immune response and tissue injury, determine the course outcome cystic fibrosis (CF) lung disease. As disease progresses, P. aeruginosa adapts to modifying dramatically its phenotype; however, it remains unclear whether how bacterial adaptive variants their persistence influence pathogenesis development. Using in vitro murine models infection, we showed that CF-adaptive shaped innate...
Trimethoprim-sulfamethoxazole (SXT) is a possible alternative for the treatment of community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) due to susceptibility most MRSA strains drug. However, after long-term with SXT, thymidine-dependent (TD) SXT-resistant small-colony variants (SCVs) emerge. In TD-SCVs, mutations thymidylate synthase ([TS] thyA) occur. Until now, it has never been systematically investigated that SXT triggering induction and/or selection...
Lung infection by Burkholderia species, in particular cenocepacia, accelerates tissue damage and increases post-lung transplant mortality cystic fibrosis patients. Host-microbe interplay largely depends on interactions between pathogen-specific molecules innate immune receptors such as Toll-like receptor 4 (TLR4), which recognizes the lipid A moiety of bacterial lipopolysaccharide (LPS). The human TLR4·myeloid differentiation factor 2 (MD-2) LPS complex is strongly activated hexa-acylated...
Abstract Resistance and tolerance mechanisms participate to the interplay between host pathogens. IL-17-mediated response has been shown be crucial for resistance respiratory infections, whereas its role in during chronic airway colonization is still unclear. Here, we investigated whether modulates of airways infection by P. aeruginosa . First, found that IL-17A levels were sustained mice at both early advanced stages confirmed these observations human samples from cystic fibrosis patients...
T cells play a prominent role in orchestrating the immune response to viral diseases, but their clinical presentation and subsequent immunity SARS-CoV-2 infection remains poorly understood. As part of population-based survey municipality Vo', Italy, conducted after initial outbreak, we sampled cell receptor (TCR) repertoires population 2 months PCR followed up positive cases 9 15 later. At months, found that 97.0% (98 101) had elevated levels TCRs associated with SARS-CoV-2. frequency...
Mycobacterium abscessus is the etiological agent of severe pulmonary infections in vulnerable patients, such as those with cystic fibrosis (CF), where it represents a relevant cause morbidity and mortality. Treatment caused by M. remains extremely difficult, this species resistant to most classes antibiotics, including macrolides, aminoglycosides, rifamycins, tetracyclines, β-lactams. Here, we show that apoptotic body like liposomes loaded phosphatidylinositol 5-phosphate (ABL/PI5P) enhance...
Pseudomonas aeruginosa is a common cause of healthcare-associated infections including pneumonia, bloodstream, urinary tract, and surgical site infections. The clinical outcome P. may be extremely variable among individuals at risk patients affected by cystic fibrosis. However, factors for infection remain largely unknown. To identify track the host influencing lung infections, inbred immunocompetent mouse strains were screened in pneumonia model system. A/J, BALB/cJ, BALB/cAnNCrl,...
Nontypeable Haemophilus influenzae (NTHi) is commonly isolated from airways of patients suffering chronic respiratory diseases, such as COPD or cystic fibrosis (CF). However, to what extent NTHi long-term infection contributes the lung inflammatory burden during airway disease still controversial. Here, we exploited human samples a small cohort CF and found that chronically infected with had significantly higher levels interleukin (IL)-8 CXCL1 than those who were not infected. To better...
Mycobacterium abscessus causes severe disease in patients with cystic fibrosis. Little is known M . about the roles of small regulatory RNAs (sRNA) gene regulation. We show that sRNA B11 controls expression and virulence-associated phenotypes this pathogen. deletion from smooth strain ATCC_19977 produced a rough strain, increased pro-inflammatory signaling virulence multiple infection models, resistance to antibiotics. Examination clinical isolate cohorts identified isolates mutations or...
Pseudomonas aeruginosa is an opportunistic pathogen and causes a wide range of acute chronic infections. P. infections are kept in check by effective immune surveillance the healthy host, while any imbalance or defect normal response can manifest disease. Invasive infection immunocompromised patients mediated potent extracellular cell bound bacterial virulence factors. Life‐threatening cystic fibrosis maintained pathogenic variants that contribute to evade detection clearance system. Here,...
The mechanisms governing the epidemiology dynamics and success determinants of a specific healthcare-associated methicillin-resistant S. aureus (HA-MRSA) clone in hospital settings are still unclear. Important epidemiological changes have occurred Europe since 2000 that been related to appearance ST22-IV clone. Between 2006 2010, we observed establishment displacing predominant Italian clone, ST228-I, large university hospital. To investigate factors associated with successful spread...