A5038135757- Glioma Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Mycobacterium research and diagnosis
- Chemokine receptors and signaling
- Ion channel regulation and function
- Cancer-related gene regulation
- Hedgehog Signaling Pathway Studies
- Immune Response and Inflammation
- Tuberculosis Research and Epidemiology
- Cancer Cells and Metastasis
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Fish Biology and Ecology Studies
- Genomics and Chromatin Dynamics
- Peroxisome Proliferator-Activated Receptors
- Brain Metastases and Treatment
- Marine and fisheries research
- Infectious Diseases and Mycology
- Ubiquitin and proteasome pathways
- Renal and Vascular Pathologies
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
Toscana Life Sciences
2025
University of Turin
1991-2024
University of Bologna
2008-2023
San Raffaele University of Rome
2018-2022
University Hospital of Zurich
2016-2022
Agostino Gemelli University Polyclinic
2016-2022
Klinikum Brandenburg
2021
Brandenburg University of Technology Cottbus-Senftenberg
2021
University of Potsdam
2021
Jena University Hospital
2021
We investigated in vitro the properties of selected populations cancer stem-like cells defined as tumorospheres that were obtained from human glioblastoma. also assessed their potential and capability differentiating into mature central nervous system. In vivo, tumorigenicity was confirmed after transplantation brain non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. The angiogenic glioblastoma-derived grown adherent revealed by evaluating release factors such vascular...
The idiopathic inflammatory myopathies are characterized by antibody- or cell-mediated immune response against unknown muscle tissue antigens. In these diseases a cellular infiltrate, composed of T and B lymphocytes, macrophages NK cells, may invade with gradient from the perivascular space to endomysial compartment. Muscle cells be actively involved in processes mononuclear cell recruitment activation blood stream areas inflammation. order verify this hypothesis, cultured human myoblasts...
Whereas the signaling function of interleukin 1 (IL-1) receptor type I (IL-1R I) has been well documented, II "receptor" suggested to act as a decoy target for this cytokine. Since IL-1 may represent key immunomodulatory and antiinflammatory properties glucocorticoids (GC), aim study was investigate effects dexamethasone (Dex) on IL-1R expression in human polymorphonuclear leukocytes (PMN), which express predominantly molecule II). We found that Dex augments levels steady state transcripts...
Glioblastoma multiforme (GBM) is among the most deadly cancers. A number of studies suggest that a fraction tumor cells with stem cell features (Glioma Stem-like Cells, GSC) might be responsible for GBM recurrence and aggressiveness. GSC similarly to normal neural cells, can form neurospheres (NS) in vitro, seem mirror genetic original better than glioma growing adherently presence serum. Using cDNA microarray analysis we identified relevant genes biology are differentially expressed...
A retroviral construct encoding polyoma middle-sized T antigen was used to generate transformed endothelial cell lines from heart (H5V), brain (B9V), and whole-embryo (E10V) of C57BL/6 mice. When injected into syngeneic recipients, H5V the less studied B9V E10V cells caused vascular tumors which, depending on number inoculated, regressed or progressed, leading death host. were immunodeficient mice, observed with inocula which did not form lesions in immunocompetent recipients regression...
Glioblastomas (GBMs), the most common primary malignancies of central nervous system, are highly aggressive and heterogeneous, remain a dramatic therapeutic challenge. Markers mirroring complex molecular profile GBMs that predictive patient outcomes needed to define novel multi-targeted treatment strategies. Resistance current GBM therapies is partly due subpopulation stem-like other self-renewing cells (hereafter called glioma cancer cells, GSCC), which therefore key interest as entry...
Abstract We studied cytokine-related functional properties of four mouse endotheliomas from different anatomical sites obtained by transformation with middle T oncogene. examined mRNA expression IL-6, IL-1 alpha, macrophage-CSF, granulocyte/macrophage-CSF, and two members an emerging super-family chemotactic cytokines (JE/monocyte chemoattractant protein-1 (MCP-1) KC). Exposure to augmented or induced cytokine gene transcripts in three endothelioma lines (eEnd.1, sEnd.1, tEnd) maximal tEnd.1...
BACK: The functional organization of polarized epithelia depends mostly on adhesion molecules belonging to the integrin and cadherin families. These either recognize basement membrane components, such as laminins, or form intercellular junctions via homotypic interactions. Such tissue is often disrupted upon neoplastic transformation, resulting loss polarization cell cohesion might be a prerequisite for invasive metastatic behavior carcinomas.We studied modifications thyroid adhesive...
Pancreatic cancer stem-like cells are described by membrane expression of CD24, CD44 and ESA (epithelial-specific antigen) their capacity to grow as spheres in a serum-free medium containing well-defined growth factors. The panel four pancreatic cell lines (PANC-1, CFPAC-1, PancTu-1 PSN-1) form was tested. All with the exception developed spheres. Phenotypically, sphere-growing showed an increased vitro invasion capability. Both gene protein expressions markers metastases [CXCR4 (CXC...
Glioblastoma multiforme (GBM) is the most common and prognostically unfavorable form of brain tumor. The aggressive highly invasive phenotype these tumors makes them among anatomically damaging human cancers with a median survival less than 1 year. Although canonical Wnt pathway activation in has been historically linked to presence mutations involving key components (APC, β-catenin, or Axin proteins), an increasing number studies suggest that elevated signaling GBM initiated by several...
Mycobacterium abscessus is the etiological agent of severe pulmonary infections in vulnerable patients, such as those with cystic fibrosis (CF), where it represents a relevant cause morbidity and mortality. Treatment caused by M. remains extremely difficult, this species resistant to most classes antibiotics, including macrolides, aminoglycosides, rifamycins, tetracyclines, β-lactams. Here, we show that apoptotic body like liposomes loaded phosphatidylinositol 5-phosphate (ABL/PI5P) enhance...
The B.1.1.529 (omicron) variant has rapidly supplanted most other SARS-CoV-2 variants. Using microfluidics-based antibody affinity profiling (MAAP), we have characterized and IgG concentration in the plasma of 39 individuals with multiple trajectories infection and/or vaccination. Antibody was similar against wild-type, delta, omicron variants (
Rapid Response Systems (RRSs) are designed to assist hospitalized patients who become unstable, aiming address “failure rescue” and prevent cardiac arrests. Despite global implementation, evidence of RRS effectiveness is controversial. This study evaluates the safety an at Maggiore Hospital in Lodi, Italy, focusing on Medical Emergency Team (MET) organization. The was established 2017 using National Early Warning Score (NEWS) for monitoring. MET, consisting emergency physician one nurse,...
Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) mediated by blood-derived immune cells invading CNS. This invasion could be determined chemokines, and their role within MS-affected brain still poorly defined. We investigated expression RT-PCR protein release ELISA interferon-gamma (IFN-gamma)-inducible chemokines in human microvascular endothelial (HBMECs) astrocytes. The monokine induced IFN-gamma (Mig) behaves as homing chemokine constitutively...
Glioblastoma multiforme (GBM) is composed of heterogeneous and genetically different cells, which are highly invasive motile. The standard chemotherapeutic agent, temozolomide, affects GBM cell proliferation but generally unable to prevent tumor recurrence. Hedgehog pathway activation has been reported be relevant in pharmacological modulators have identified. We report that by growing a commercially available recurrent line (DBTRG-05MG) without serum the presence defined growth factors; we...
On the basis of trawl survey data collected during MEDITS project (1994-1999), distribution Mediterranean hake populations was described in coastal areas corresponding to about three quarters both latitudinal and longitudinal extensions Sea, 0-800 m range. Abundance biomass indices (in terms number kg per km2) are presented 15 geographical sectors 40 subareas from Alborán Sea Aegean Sea. A statistical analysis by generalized linear modelling performed on such main national blocks (Greece,...
Pulmonary infections caused by Mycobacterium abscessus (MA) have increased over recent decades, affecting individuals with underlying pathologies such as chronic obstructive pulmonary disease, bronchiectasis and, especially, cystic fibrosis. The lack of a representative and standardized model infection in mice has limited steps forward the field MA infection. To overcome this challenge, we refined method agar beads to establish immunocompetent mice. We evaluated bacterial count, lung...