A5034583993- HER2/EGFR in Cancer Research
- Wnt/β-catenin signaling in development and cancer
- Radiopharmaceutical Chemistry and Applications
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Ferrocene Chemistry and Applications
- Medical Imaging Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Cell Adhesion Molecules Research
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Advanced Breast Cancer Therapies
- Machine Learning in Bioinformatics
- Lung Cancer Treatments and Mutations
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
Menarini Group (Italy)
2019-2023
IRBM Science Park
2019
Siena Biotech (Italy)
2009-2015
Agency for Science, Technology and Research
2013
National Cancer Institute
2003-2005
Center for Cancer Research
2003-2005
Science Applications International Corporation (United States)
2005
National Institutes of Health
2002-2005
Sapienza University of Rome
1998
Reestablishment of metastasis suppressor gene expression may constitute a therapeutic strategy for high-risk breast cancer patients. We previously showed that medroxyprogesterone acetate (MPA), progestin has been tested as treatment advanced cancer, elevates the Nm23-H1 in hormone receptor-negative metastatic human carcinoma cell lines vitro via glucocorticoid receptor-based mechanism. Here, we whether MPA inhibits colonization line vivo.We soft-agar colony-forming efficiency untransfected...
Metastatic disease is a significant contributor to cancer patient mortality. We previously reported that the Kinase Suppressor of Ras1 (KSR1) scaffold protein for Erk mitogen-activated kinase pathway coimmunoprecipitated metastasis suppressor Nm23-H1. now hypothesize altered expression levels Nm23-H1 influence binding properties, stability, and function KSR1 scaffold. Increased coimmunoprecipitation Hsp90 with was observed in either stable or transient transfectants nm23-H1 MDA-MB-435 human...
Glioblastoma multiforme (GBM) is the most common and prognostically unfavorable form of brain tumor. The aggressive highly invasive phenotype these tumors makes them among anatomically damaging human cancers with a median survival less than 1 year. Although canonical Wnt pathway activation in has been historically linked to presence mutations involving key components (APC, β-catenin, or Axin proteins), an increasing number studies suggest that elevated signaling GBM initiated by several...
CD205 is a type I transmembrane glycoprotein and member of the C-type lectin receptor family. Analysis by mass spectrometry revealed that was robustly expressed highly prevalent in variety solid malignancies from different histotypes. IHC confirmed increased expression pancreatic, bladder, triple-negative breast cancer (TNBC) compared with corresponding normal tissues. Using immunofluorescence microscopy, rapid internalization antigen observed. These results supported development...
Abstract Purpose Dysregulation of the PI3K pathway is one most common events in breast cancer. Here we investigate activity inhibitor MEN1611 at both molecular and phenotypic levels by dissecting comparing its profile efficacy HER2 + cancer models with other inhibitors. Methods Models different genetic backgrounds were used to pharmacological against In vitro studies evaluated cell viability, signaling, death upon treatment MEN1611. vivo compound was investigated line- patient-derived...
Sarcomas are mesenchymal tumors showing high molecular heterogeneity, reflected at the histological level by existence of more than fifty different subtypes. Genetic and epigenetic evidences link aberrant activation Wnt signaling to growth progression human sarcomas. This phenomenon, mainly accomplished autocrine loop activity, is sustained gene amplification, over-expression ligands co-receptors or silencing endogenous antagonists. We previously showed that pharmacological inhibition...
Abstract DigesTip is a new device for in‐solution protein digestion, based on patent pending technology, able to immobilize enzymes (trypsin, in this case) solid surface, keeping their activity preserved. standard pipette tip, usable both by human and robots. Its main performances are: very short digestion time (1 min) usability with low sample concentrations (5 μg/mL). obtains clear signal MS measurements its usage rules out several preparative steps.
Confidence in mechanism: Creating a more holistic understanding of disease pathophysiology and an early confidence the mechanism under investigation could help facilitate selection not only most appropriate targets but also best mechanisms for intervention how to select optimise compounds. Drug target candidate are two key decision points within drug discovery process which all companies use certain criteria make decisions on accept into their pipelines compounds will pass development. These...
The murine <i>Htf9-a/RanBP1</i> and <i>Htf9-c</i> genes are divergently transcribed from a shared TATA-less promoter. Transcription of both is initiated on complementary DNA strands controlled by cell cycle-dependent mechanisms. bidirectional promoter harbors genomic footprint flanking the major transcription start site genes. Transient assays showed that footprinted element important for Protein-binding experiments antibody indicated members retinoid X receptor family interact with...
Lung cancer remains the leading cause of cancer-related death worldwide. Targeted therapies with tyrosine kinase inhibitors (TKIs) result in improvement survival for non-small cell lung (NSCLC) activating mutations epidermal growth factor receptor (EGFR). Unfortunately, most patients who initially respond to EGFR-TKI ultimately develop resistance therapy, resulting progression and relapse. Combination therapy is today a common strategy treatment tumors increase success rate, improve outcome...
Adoptive transfer of T lymphocytes (ACT) engineered with T-cell receptors (TCRs) known antitumor specificity is an effective therapeutic strategy. However, a major constraint ACT the unpredictable interference endogenous TCR α and β chains in pairing transduced TCR. This effect reduces efficacy genetically modified primary cells carries risk generating novel reactivities unintended functional consequences. Here, we show powerful approach to overcome these limitations. We mutations...
<div>Abstract<p>Glioblastoma multiforme (GBM) is the most common and prognostically unfavorable form of brain tumor. The aggressive highly invasive phenotype these tumors makes them among anatomically damaging human cancers with a median survival less than 1 year. Although canonical Wnt pathway activation in has been historically linked to presence mutations involving key components (APC, β-catenin, or Axin proteins), an increasing number studies suggest that elevated signaling...
<div>Abstract<p>CD205 is a type I transmembrane glycoprotein and member of the C-type lectin receptor family. Analysis by mass spectrometry revealed that CD205 was robustly expressed highly prevalent in variety solid malignancies from different histotypes. IHC confirmed increased expression pancreatic, bladder, triple-negative breast cancer (TNBC) compared with corresponding normal tissues. Using immunofluorescence microscopy, rapid internalization antigen observed. These results...
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<p>PDF file - 94K, Consequences of inhibition WNT signaling in glioblastoma cells: a less proliferative and tumorigenic phenotype.</p>
<p>PDF file - 112K, Average body weight graph.</p>
<p>PDF file - 32K, Effects of different compound AXIN stabilizers on TNKS1 and TNKS2.</p>