A5060717808- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Brucella: diagnosis, epidemiology, treatment
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Liver Diseases and Immunity
- Synthesis and Reactions of Organic Compounds
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Polyomavirus and related diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Influenza Virus Research Studies
- Silymarin and Mushroom Poisoning
- Osteomyelitis and Bone Disorders Research
Vita-Salute San Raffaele University
2014-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2022
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2021-2022
Universidad Católica de Córdoba
1963
Severe coronavirus disease 2019 (COVID-19) is characterized by overproduction of immune mediators, but the role interferons (IFNs) type I (IFN-I) or III (IFN-III) families remains debated. We scrutinized production IFNs along respiratory tract COVID-19 patients and found that high levels IFN-III, to a lesser extent IFN-I, characterize upper airways with viral burden reduced risk severity. Production specific not members denotes mild pathology efficiently drives transcription genes protect...
SARS-CoV-2 fine-tunes the interferon (IFN)-induced antiviral responses, which play a key role in preventing coronavirus disease 2019 (COVID-19) progression. Indeed, critically ill patients show an impaired type I IFN response accompanied by elevated inflammatory cytokine and chemokine levels, responsible for cell tissue damage associated multi-organ failure. Here, early interaction between immune cells was investigated interrogating vitro human peripheral blood mononuclear (PBMC)-based...
Abstract The ongoing coronavirus disease 2019 pandemic has forced the clinical and scientific community to try drug repurposing of existing antiviral agents as a quick option against severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2). Under this scenario, interferon (IFN) β-1a, whose potential is already known, which currently used in management multiple sclerosis, may represent candidate. In report, we demonstrate that IFN-β-1a was highly effective inhibiting vitro SARS-CoV-2...
Plenty of serologic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed so far, thus documenting the importance evaluating relevant features immune response to this viral agent. The performance these assays is currently under investigation. Amongst them, LIAISON® SARS-CoV-2 S1/S2 IgG by DiaSorin and Elecsys Anti-SARS-CoV-2 cobas® Roche are used laboratory medicine hospital departments in Italy many other countries. In present study, we firstly compared...
Abstract Since early May 2022, some monkeypox virus (MPXV) infections have been reported from countries where the disease is not endemic. Within 2 months, number of patients has increased extensively, becoming most considerable MPXV outbreak described. Smallpox vaccines demonstrated high efficacy against MPXVs in past and are considered a crucial control measure. However, viruses isolated during current carry distinct genetic variations, cross‐neutralizing capability antibodies remains to be...
Abstract A growing number of emerging SARS-CoV-2 variants is being identified worldwide, potentially impacting the effectiveness current vaccines. We report data obtained in several Italian regions involved variant monitoring from beginning epidemic and spanning period October 2020 to March 2021.
Background: Studies reporting the long-term humoral response after receiving BNT162b2 COVID-19 vaccine are important to drive future vaccination strategies. Yet, available literature is scarce. Covidiagnostix a multicenter study designed assess antibody in >1000 healthcare professionals (HCPs) who received vaccine. Methods: Serum was tested at time-0 (T0), before first dose, T1, T2, and T3, respectively, 21, 42, 180 days T0. Antibodies against SARS-CoV-2 nucleocapsid-protein were measured...
The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA can recover full-length transcripts, greatly simplifying the assembly of structurally RNAs. However, these techniques do not detect 5' cap, thus preventing reliable identification quantification full-length, coding transcript models. Here we used Nanopore ReCappable Sequencing...
The purpose of this study was to evaluate the efficacy and safety Moderna-1273 mRNA vaccine for SARS-CoV-2 in patients with immune-mediated diseases under different treatments. Anti-trimeric spike protein antibodies were tested 287 rheumatic or autoimmune (10% receiving mycophenolate mofetil, 15% low-dose glucocorticoids, 21% methotrexate, 58% biologic/targeted synthetic drugs) at baseline 219 (76%) 4 weeks after second dose. Family members caretakers enrolled as controls. neutralizing serum...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive single-stranded RNA virus, engages in complex interactions with host cell proteins throughout its life cycle. While these enable the to recognize and inhibit viral replication, they also facilitate essential processes such as transcription, translation, replication. Many aspects of virus-host remain poorly understood. Here, we employed catRAPID algorithm utilized RNA-protein interaction detection coupled mass...
The SARS-CoV-2 protease (3CLpro) is one of the key targets for development efficacious drugs COVID-19 treatment due to its essential role in life cycle virus and exhibits high conservation among coronaviruses. Recent studies have shown that flavonoids, which are small natural molecules, antiviral activity against coronaviruses (CoVs), including SARS-CoV-2. In this study, we identified docking sites binding affinity several compounds, similar investigated their inhibitory towards 3CLpro...
While the SARS-CoV-2 pandemic is heavily hitting world, it of extreme importance that significant in vitro observations guide quick set up clinical trials. In this study, we evidence anti-SARS-CoV2 activity a clinically achievable hydroxychloroquine concentration maximized only when administered before and after infection Vero E6 Caco-2 cells. This suggests combined prophylactic therapeutic use may be effective limiting viral replication patients.
Abstract SARS-CoV-2 fine-tunes the interferon (IFN)-induced antiviral responses, which play a key role in preventing coronavirus disease 2019 (COVID-19) progression. Indeed, critically ill patients show an impaired type I IFN response accompanied by elevated inflammatory cytokine and chemokine levels, responsible for cell tissue damage associated multi-organ failure. Here, early interaction between immune cells was investigated interrogating vitro human peripheral blood mononuclear...