A5042442807- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Protein Structure and Dynamics
- vaccines and immunoinformatics approaches
- Protein Kinase Regulation and GTPase Signaling
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Protein Tyrosine Phosphatases
- Neurobiology and Insect Physiology Research
- Pediatric Pain Management Techniques
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Cytokine Signaling Pathways and Interactions
- RNA and protein synthesis mechanisms
- Dermatology and Skin Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Migraine and Headache Studies
- Viral gastroenteritis research and epidemiology
- Nerve injury and regeneration
Dompé (Italy)
2022-2024
Universitat de Barcelona
2013-2022
National Cancer Institute
2020
European Molecular Biology Laboratory
2017-2019
European Molecular Biology Organization
2017
Institute for Research in Biomedicine
2013
Abstract COVID-19 has rapidly spread all over the world, progressing into a pandemic. This situation urgently impelled many companies and public research institutes to concentrate their efforts on for effective therapeutics. Here, we outline strategies targets currently adopted in developing vaccine against SARS-CoV-2. Based previous evidence experience with SARS MERS, primary focus been Spike protein, considered as ideal target immunotherapies.
Mechanical allodynia is a major symptom of neuropathic pain whereby innocuous touch evokes severe pain. Here we identify population peripheral sensory neurons expressing TrkB that are both necessary and sufficient for producing from light after nerve injury in mice. Mice which TrkB-Cre-expressing ablated less sensitive to the lightest under basal conditions, fail develop mechanical model Moreover, selective optogenetic activation these marked nociceptive behavior. Using phototherapeutic...
c-Src is a non-receptor tyrosine kinase involved in numerous signal transduction pathways. The kinase, SH3 and SH2 domains of are attached to the membrane-anchoring SH4 domain through flexible Unique domain. Here we show intra- intermolecular interactions involving suggesting presence previously unrecognized additional regulation layer c-Src. We have characterized lipid binding by domains, their intramolecular interaction its allosteric modulation SH3-binding peptide or Calcium-loaded...
Abstract Intrinsically disordered regions (IDRs) are preferred sites for post‐translational modifications essential regulating protein function. The enhanced local mobility of IDRs facilitates their observation by NMR spectroscopy in vivo. Phosphorylation events can occur at multiple and respond dynamically to changes kinase–phosphatase networks. Here we used real‐time study the effect kinases phosphatases present Xenopus oocytes egg extracts on phosphorylation state “unique domain” c‐Src....
COVID-19 is a highly infectious disease caused by newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into pandemic. This unprecedent emergency stressed the significance of developing effective therapeutics to fight current and future outbreaks. The receptor-binding domain (RBD) SARS-CoV-2 surface Spike protein main target for vaccines represents helpful "tool" produce neutralizing antibodies or diagnostic kits. In this work, we provide detailed characterization native RBD...
Abstract The membrane-anchored Src tyrosine kinase is involved in numerous pathways and its deregulation human cancer. Our knowledge on regulation relies crystallography, which revealed intramolecular interactions to control active conformations. However, contains a N-terminal intrinsically disordered unique domain (UD) whose function remains unclear. Using NMR, we reported that UD forms an fuzzy complex involving conserved region with lipid-binding capacity named Unique Lipid-Binding Region...
Tracking the genetic variability of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) is a crucial challenge. Mainly to identify target sequences in order generate robust vaccines and neutralizing monoclonal antibodies, but also track viral temporal geographic evolution mine for variants associated with reduced or increased disease severity. Several online tools bioinformatic phylogenetic analyses have been released, main interest lies Spike protein, which pivotal element current...
Abstract Nerve growth factor (NGF) and its receptors TrkA p75 play a key role in the development function of peripheral nociceptive neurons. Here, we describe novel technology to selectively photoablate TrkA-positive nociceptors through delivery phototoxic agent coupled an engineered NGF ligand subsequent near-infrared illumination. We demonstrate that this approach allows for on demand localized reversal pain behaviors mouse models acute, inflammatory, neuropathic, joint pain. To target...
Abstract Gene delivery using vector or viral-based methods is often limited by technical and safety barriers. A promising alternative that circumvents these shortcomings the direct of proteins into cells. Here we introduce a non-viral, ligand-mediated protein system capable selectively targeting primary skin cells in-vivo . Using orthologous self-labelling tags chemical cross-linkers, conjugate large to ligands bind their natural receptors on surface keratinocytes. Targeted CRE-mediated...
Intrinsically disordered proteins \(IDPs) play important roles in a multitude of biological process, especially the regulation signal transduction pathways.Many IDPs are implicated several diseases such as cancer, diabetes, neurodegenerative and others.We have developed detailed protocol for purifying intrinsically Unique domain human non-receptor tyrosine kinase c-Src.Moreover, here we introduce two additional techniques that been used to assess capability protein binding lipids: simple...
Abstract COVID-19 is a highly infectious disease caused by newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into pandemic. This unprecedent emergency stressed the significance of developing effective therapeutics to fight current and future outbreaks. The receptor-binding domain (RBD) SARS-CoV-2 surface Spike protein main target for vaccines represents helpful “tool” produce neutralizing antibodies or diagnostic kits. In this work, we provide detailed characterization...
In addition to its critical role in neurogenesis, brain-derived neurotrophic factor (BDNF) modulates pain and depressive behaviors. a translational perspective, we tested the anti-migraine activity of highly purified characterized recombinant human BDNF (rhBDNF) an animal model cephalic based on chronic intermittent NTG administration (five total injections over nine days), used mimic recurrence attacks given period. To achieve this, assessed effects two doses rhBDNF (40 80 µg/kg)...
Thus far, no manufacturing process able to support industrialization has been reported for the recombinant human brain-derived neurotrophic factor (rhBDNF). Here, we described setup of a new protocol its production in Escherichia coli (E. coli) and purification homogeneity. A synthetic gene, codifying neurotrophin precursor, was inserted into an E. expression vector transformed BL21 (DE3) strain. The protein accumulates, at high yields, inclusion bodies. With developed strategy, more than...
Abstract The COVID-19 pandemic caused by the β-coronavirus SARS-CoV-2 has made development of safe and effective vaccines a critical global priority. To date, four have already been approved European American authorities for preventing but additional vaccine platforms with improved supply logistics profiles remains pressing need. Here we report preclinical evaluation novel candidate based on electroporation engineered, synthetic cDNA encoding viral antigen in skeletal muscle, technology...
Abstract Nerve growth factor (NGF) and its receptors TrkA p75 play a key role in the development function of peripheral nociceptive neurons. Here we describe novel technology to selectively photoablate positive nociceptors through delivery phototoxic agent coupled an engineered NGF ligand subsequent near infrared (NIR) illumination. We demonstrate that this approach allows for on demand localized reversal pain behaviors mouse models acute, inflammatory, neuropathic joint pain. To target...
Abstract Severe coagulopathy has been observed at the level of microcirculation in several organs including lungs, heart and kidneys patients with COVID-19, a minority subjects receiving SARS-CoV-2 vaccine. Various mechanisms have implicated these effects, increases circulating neutrophil extracellular traps, excessive inflammation, endothelial dysfunction. Even if correlation between infection by upregulation coagulation cascade components established lung, no direct proofs yet provided...