A5057659086- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related Molecular Pathways
- Catalysis for Biomass Conversion
- Catalytic Alkyne Reactions
- Ubiquitin and proteasome pathways
- Surfactants and Colloidal Systems
- Biofuel production and bioconversion
- Asymmetric Hydrogenation and Catalysis
- Intracerebral and Subarachnoid Hemorrhage Research
- Electrochemical sensors and biosensors
- RNA Interference and Gene Delivery
- Neurological Disease Mechanisms and Treatments
- Fungal and yeast genetics research
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Synthetic Organic Chemistry Methods
- Neuroinflammation and Neurodegeneration Mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- Cardiac Ischemia and Reperfusion
- Pharmacogenetics and Drug Metabolism
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Catalytic Cross-Coupling Reactions
- Anesthesia and Neurotoxicity Research
Universidad de Salamanca
1985-2024
Instituto de Biología Funcional y Genómica
2018-2024
Instituto de Investigación Biomédica de Salamanca
2019-2024
Hospital Clínico San Carlos
2024
Centro de Investigación Biomédica en Red
2023
Centro de Investigación en Red en Enfermedades Cardiovasculares
2023
Instituto de Salud Carlos III
2023
Metropolitan University
2019
Consejo Superior de Investigaciones Científicas
2018
Universidad de Oviedo
2007-2016
Perturbations of the yeast cell wall trigger a repair mechanism that reconfigures its molecular structure to preserve integrity. To investigate this mechanism, we compared global gene expression in five mutant strains, each bearing mutation (i.e. fks1, kre6, mnn9, gas1, and knr4 mutants) affects different manner construction. Altogether, 300 responsive genes were kept based on high stringency criteria during data processing. Functional classification these differentially expressed showed...
Transplanted neurons derived from stem cells have been proposed to improve function in animal models of human disease by various mechanisms such as neuronal replacement. However, whether the grafted receive functional synaptic inputs recipient's brain and integrate into host neural circuitry is unknown. Here we studied cortical induced pluripotent after transplantation stroke-injured rat cerebral cortex. Using rabies virus-based trans-synaptic tracing method immunoelectron microscopy,...
Nowadays, the design of sustainable processes applicable at industrial scale is highly desirable due to environmental reasons. The use biocatalytic reactions carry out oxidative transformations one possible strategies framed in here mildness and usually high selectivities achieved with these methods. Anyway, while implementing this type system several drawbacks must be overcome order obtain efficient setups. Historically, main issues has been cofactor-dependency biocatalysts active. Herein,...
Lactobacillus brevis ADH (LBADH) is an alcohol dehydrogenase that commonly employed to reduce alkyl or aryl ketones usually bearing a methyl, ethyl chloromethyl as small ketone substituent the corresponding (R)-alcohols. Herein we have tested series of 24 acetophenone derivatives differing in their size and electronic properties for reduction employing LBADH. After plotting relative activity against measured substrate volumes observed apart from other effects must be responsible obtained....
Intracerebral hemorrhage (ICH) is a devastating subtype of stroke that lacks effective therapy and reliable prognosis. Neovascularization following ICH an essential compensatory response mediates brain repair modulates the clinical outcome patients. However, mechanism dictates this process unknown. Bone marrow-derived endothelial progenitor cells (EPCs) promote contribute to ischemia-induced neovascularization. The human Tp53 gene harbors common single-nucleotide polymorphism (SNP) at codon...
Flying PIKATs: The stereoselective oxidation of several sulfides linked to the enantioselective different sec-alcohols are combined in a parallel interconnected kinetic asymmetric transformation (PIKAT) fashion. Furthermore, cofactor concentration employed these processes is investigated, demonstrating high capacity performance, even at micromolar concentrations mediator.
Parallel interconnected kinetic asymmetric transformations were performed in order to obtain enantioenriched derivatives starting from a set of racemic or prochiral compounds. Thus, one-pot reaction using two redox biocatalysts (a BVMO and an ADH) catalytic amount cofactor that acts as mediator, ketones, sulfoxides, sec-alcohols concurrently obtained strict parallel way, minimising the quantity reagents employed. By selecting appropriate biocatalysts, this methodology represents potential...
Type I Baeyer-Villiger monooxygenases (BVMOs) strongly prefer NADPH over NADH as an electron donor. In order to elucidate the molecular basis for this coenzyme specificity, we have performed a site-directed mutagenesis study on phenylacetone monooxygenase (PAMO) from Thermobifida fusca. Using sequence alignments of type BVMOs and crystal structures PAMO cyclohexanone in complex with NADP(+), identified four residues that could interact 2'-phosphate moiety PAMO. The revealed conserved R217 is...
BVMOs make a play: The dynamic kinetic resolution of racemic α-alkyl-β-ketoesters was performed through selective Baeyer–Villiger oxidation employing different monooxygenases (BVMOs) in mild basic media. product diesters were obtained with excellent yields and enantioselectivities, used as precursors for optically active α-hydroxy esters.
The cell wall is an essential structure that preserves the osmotic integrity of fungal cells and determines cellular morphology during developmental programs. high number different components demands a variety processes to deliver precursors synthetic proteins proper location at right time for development modification. Here,the specificity mechanisms regulate temporal spatial localisation sites polarised growth in Saccharomyces cerevisiae investigated. For this purpose, Crh2p,...
Abstract Phenylacetone monooxygense (PAMO) from Thermobifida fusca was employed for the asymmetric oxidation of thioanisole (sulfooxidation) and racemic 2‐phenylpropionaldehyde (Baeyer–Villiger oxidation). A pH dependence enantioselectivity observed in both cases. Two different residues, with p K a values 7.8±0.2 9.2±0.2, appeared to be responsible effects on PAMO enantioselectivity. The protonation Arg337 FAD:C4a‐hydroperoxide/FAD:C4a‐peroxide equilibrium were identified as major factors...
4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB was employed in the presence of a weak anion exchange resin to perform dynamic kinetic resolutions racemic benzyl ketones with high conversions and good optical purities. Different parameters that affect efficiency enzymatic Baeyer–Villiger oxidation racemisation were analyzed order optimize activity selectivity biocatalytic system.
Lysine-based gemini surfactants with mono-unsaturated tails transfect without helper lipid because of the ability lipoplex to change morphology pH.
The Fizzy-related protein 1 (Fzr1) gene encodes Cdh1 protein, a coactivator of the E3 ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). Previously, we found that genetic ablation Fzr1 promotes death neural progenitor cells leading to neurogenesis impairment and microcephaly in mouse. To ascertain possible translation these findings humans, searched for mutations 390 whole exomes sequenced trio individuals showing neurodevelopmental disorders compatible with origin. We novel...
Ischemia-induced WRAP53 nuclear accumulation promotes DNA damage repair resulting in functional recovery after stroke.
The synthesis of three novel cationic gemini surfactants (10, 12, and 14) based on tartaric acid appended with biocompatible palmitoyl tails head groups is described, their aggregation in water, monolayer behaviour, DNA binding, gene transfection activities are reported. studies showed that the molecular area determined by group, as it increased going from ethylenediamine group 10 via lysine 12 to combined lysine/ethylenediamine 14. Electron microscopy smaller (10 12) form plate-like...
Background and Purpose— The E3 ubiquitin ligase MDM2 (murine double minute 2) is the main negative regulator of p53 protein—a key player in neuronal apoptosis after ischemia. A functional single-nucleotide polymorphism human gene promoter (rs2279744) regulates protein expression. We investigated whether SNP309, by controlling p53-mediated apoptosis, determines outcome stroke. Methods— Primary cortical neurons were subjected to oxygen glucose deprivation. Mice ischemic (transient middle...