A5017388420- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Reactions
- Carbohydrate Chemistry and Synthesis
- Pharmacogenetics and Drug Metabolism
- Biofuel production and bioconversion
- Steroid Chemistry and Biochemistry
- Chemical Reactions and Isotopes
- Metal-Catalyzed Oxygenation Mechanisms
- Chemical Synthesis and Analysis
- Enzyme-mediated dye degradation
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Plant-derived Lignans Synthesis and Bioactivity
- Catalysis for Biomass Conversion
- Electrochemical sensors and biosensors
- Sulfur-Based Synthesis Techniques
- Oxidative Organic Chemistry Reactions
- Phytochemistry and Biological Activities
- Lignin and Wood Chemistry
- Drug Transport and Resistance Mechanisms
- Phytochemicals and Antioxidant Activities
- Algal biology and biofuel production
- Allelopathy and phytotoxic interactions
Istituto di Chimica Biomolecolare
1990-2025
Istituto di Scienze e Tecnologie Chimiche "Giulio Natta"
2021-2024
National Research Council
2019-2024
Institute of Chemistry of Molecular Recognition
2009-2019
Politecnico di Milano
2015
University of Insubria
2014-2015
University of Milan
1997-2002
Institute for Macromolecular Studies
1995
University of Trieste
1993
In this work, six different deep eutectic solvent (DES) treatments—five acidic (natural organic acid–choline chloride) and one alkaline (K2CO3–glycerol)—were used compared in delignification nanofibrillation of agricultural by-products from wheat straw, corn stalk, rapeseed stem. The DES treatments were performed at 100 °C for 8 h, 16 80 24 h. yield cellulose lignin fractions was obtained gravimetrically, a more detailed composition the h treatment. All samples further nanofibrillated,...
We previously purified a new esterase from the thermoacidophilic eubacterium Bacillus acidocaldarius whose N-terminal sequence corresponds to an open reading frame (ORF3) reported show homology with mammalian hormone-sensitive lipase (HSL)-like group of esterase/lipase family. To compare biochemical properties this thermophilic enzyme those homologous mesophilic and psychrophilic members HSL group, overexpression system in Escherichia coli was established. The protein, expressed soluble...
As interest in lignocellulosic biomass as a feedstock for conversion into biofuels is steadily growing, analysis of its components becomes ever more important. The complete chemical composition waste hemp hurds from the industrial variety "Carmagnola" has been determined to optimize utilization raw material. results show that contain 44.0% alpha-cellulose, 25.0% hemicellulose, and 23.0% lignin major components, along with 4.0% extractives (oil, proteins, amino acids, pectin) 1.2% ash....
Abstract Fractionation of hemp hurds into its three main components, cellulose, hemicellulose, and lignin, was carried out using organosolv pretreatment. The effect processing parameters, such as temperature, catalyst concentration, reaction time, methanol (MeOH) on the dissolution recovery hemicellulose lignin determined. More than 75 % total removed in a single step with low amounts degradation products under following conditions: 165 °C, 3 H 2 SO 4 , 20 min 45 MeOH. Enzymatic hydrolysis...
This review article briefly introduces the applications of bacterial cyclohexanone monooxygenases to enantioselective oxidations organic sulfur compounds sulfoxides. High enantioselectivities are observed in sulfoxidation alkyl aryl sulfides, disulfides, dialkyl cyclic and acyclic 1,3-dithioacetals. The oxidation sulfides with flavin dependent microorganisms extends synthetic interest this class enzymes.
Enzymatic hydrolysis of oleuropein carried out in a NMR tube, gave two diastereoisomeric aglycones, which have been identified and characterized by proton carbon experiments the same aqueous reaction medium. The stereostructures, assigned to aglycones NOESY spectroscopy molecular dynamics calculation are those diastereoisomers, 4a 4b.
AbstractThis review pinpoints the strategies which can be employed to improve enantio- and diasteroselectivity of hydrolytic enzymes, i.e. esterases, proteases, lipases. The influence variations reactants, — enzyme substrate conditions kinetics, medium, temperature, pH on chiral recognition process is discussed with examples from recent literature.Key Words: EsteraseProteaseLipaseSelectivity
Natural compounds are increasingly favored over synthetic ones for their lower environmental impact. However, extraction and characterization processes typically rely on harsh conditions conventional solvents, which unsustainable cause pollution. This study aimed to develop an eco-friendly method isolate evaluate the antimicrobial properties of bioactive from Chlorella sorokiniana. Using dimethyl carbonate (DMC), methoxycyclopentane (CPME), butan-2-one (MEK) as green solvents alongside...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegioselective acylation of bile acid derivatives with Candida cylindracea lipase in anhydrous benzeneSergio Riva, Roberto Bovara, Gianluca Ottolina, Francesco Secundo, and Giacomo CarreaCite this: J. Org. Chem. 1989, 54, 13, 3161–3164Publication Date (Print):June 1, 1989Publication History Published online1 May 2002Published inissue 1 June 1989https://pubs.acs.org/doi/10.1021/jo00274a036https://doi.org/10.1021/jo00274a036research-articleACS...
A broader exploitation of enzymes in organic synthesis can be achieved by increasing their tolerance toward solvents. In this study, the stability and activity Baeyer-Villiger monooxygenases from Thermobifida fusca (PAMO) Acinetobacter sp. (CHMO) presence water miscible solvents were compared. PAMO was more stable than CHMO. The concentration solvent (v/v) at which it halved its (C(50) ) 4- to 16-fold higher that observed for For PAMO, C(50) varied 16% 55% followed destabilizing order...
The performances of the unspecific peroxigenase from Agrocybe aegerita ( Aae UPO) in asymmetric sulfoxidation substituted aryl alkyl sulfides were here investigated. A small library differently sulfoxides was successfully synthesized corresponding presence UPO and H 2 O . All obtained as R )‐enantiomers, regardless substitution pattern both on aromatic ring chain, up to > 99 % conversion ee An overview about biocatalytic entries chiral is also presented form a comparison between results...