A5016541719- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Redox biology and oxidative stress
- RNA Interference and Gene Delivery
- Agricultural Economics and Practices
- T-cell and B-cell Immunology
- Nerve Injury and Rehabilitation
- Immune Cell Function and Interaction
- Livestock Management and Performance Improvement
- Single-cell and spatial transcriptomics
- Agricultural risk and resilience
- COVID-19 and Mental Health
- Glutathione Transferases and Polymorphisms
- Hemoglobinopathies and Related Disorders
- Shoulder Injury and Treatment
- Medical Imaging and Analysis
- Selenium in Biological Systems
- Spine and Intervertebral Disc Pathology
- Acute Myeloid Leukemia Research
- Agricultural Innovations and Practices
- Mesenchymal stem cell research
- Stroke Rehabilitation and Recovery
- Mental Health Treatment and Access
- SARS-CoV-2 and COVID-19 Research
Medical College and Hospital, Kolkata
2020-2025
R. G. Kar Medical College and Hospital
2020-2025
Raiganj University
2024
Assam Medical College
2024
Nepalese Army Institute of Health Services
2020-2021
Murshidabad Medical College and Hospital
2020
Bangur Institute of Neurosciences
2020
Calcutta National Medical College and Hospital
2020
Boston Children's Hospital
2010-2018
Harvard University
2012-2018
GSH is the major antioxidant and detoxifier of xenobiotics in mammalian cells. A strong decrease intracellular has been frequently linked to pathological conditions like ischemia/reperfusion injury degenerative diseases including diabetes, atherosclerosis, neurodegeneration. Although essential for survival, deleterious effects deficiency can often be compensated by thiol-containing antioxidants. Using three genetically defined cellular systems, we show here that forced expression xCT,...
Hematopoietic stem cells (HSCs) are the best-characterized tissue-specific cells, yet experimental study of HSCs remains challenging, as they exceedingly rare and methods to purify them cumbersome. Moreover, genetic tools for specifically investigating HSC biology lacking. To address this we sought identify genes uniquely expressed in within hematopoietic system develop a reporter strain that labels them. Using microarray profiling identified several with HSC-restricted expression....
Tumor cells generate substantial amounts of reactive oxygen species (ROS), engendering the need to maintain high levels antioxidants such as thioredoxin (Trx)- and glutathione (GSH)-dependent enzymes. Exacerbating oxidative stress by specifically inhibiting these types ROS-scavenging enzymes has emerged a promising chemotherapeutic strategy kill tumor cells. However, potential redundancies among various antioxidant systems may constrain this simple approach. Trx1 reductase 1 (Txnrd1) are...
A hallmark of adult hematopoiesis is the continuous replacement blood cells with limited lifespans. While active hematopoietic stem cell (HSC) contribution to multilineage foundation clinical HSC transplantation, recent reports have questioned physiological HSCs normal/steady-state hematopoiesis. Here, we use inducible lineage tracing from genetically marked and reveal robust HSC-derived This commences via defined progenitor cells, but varies substantially in between different lineages. By...
The inhibitory reversible oxidation of protein tyrosine phosphatases (PTPs) is an important regulatory mechanism in growth factor signaling. Studies on PTP have focused pathways that increase or decrease reactive oxygen species levels and thereby affect oxidation. processes involved reactivation oxidized PTPs remain largely unknown. Here the role thioredoxin (Trx) system was analyzed using a combination vitro cell-based assays. Cells lacking major Trx reductase TrxR1 ( Txnrd1 −/− ) displayed...
Background— Excessive formation of reactive oxygen species contributes to tissue injury and functional deterioration after myocardial ischemia/reperfusion. Especially, mitochondrial are capable opening the permeability transition pore, a harmful event in cardiac Thioredoxins key players defense against oxidative stress. Mutations thioredoxin reductase (thioredoxin reductase-2, Txnrd2) gene have been recently identified cause dilated cardiomyopathy patients. Here, we investigated whether is...
Homeostasis of short-lived blood cells is dependent on rapid proliferation immature precursors. Using a conditional histone 2B-mCherry-labeling mouse model, we characterize hematopoietic stem cell (HSC) and progenitor dynamics in steady state following several types induced stress. HSC transplantation into lethally irradiated mice fundamentally different not only from native hematopoiesis but also other stress contexts. Whereas promoted sustained, long-term HSCs, both cytokine-induced...
The selenoenzyme glutathione peroxidase 4 (GPx4) has been described to control specific cyclooxygenases (COXs) and lipoxygenases (LOXs) that exert substantiated functions in tumor growth angiogenesis. Therefore, we hypothesized a putative regulatory role of GPx4 during progression created transformed murine embryonic fibroblasts with inducible disruption GPx4. inactivation caused rapid cell death vitro, which could be prevented either by lipophilic antioxidants or 12/15-LOX-specific...
Recent evidence points to the embryonic emergence of some tissue-resident innate immune cells, such as B-1a lymphocytes, prior and independently hematopoietic stem cells (HSCs). However, whether full repertoire HSCs initially includes these unique lineages has been difficult assess due lack clonal assays that identify HSC precursor (pre-HSC) potential. Here, by combining index sorting single hemogenic precursors with in vitro maturation transplantation assays, we analyze emerging pre-HSCs at...
Abstract Background COVID-19 pandemic has created unprecedented health and economic impact. Psychological stress, anxiety depression are affecting not only patients but also professionals, general population. Fear of contracting COVID-19, forced restrictive social measures, hardship causing mental trauma. Nepal is a developing country from South Asia where the still evolving. This online survey been carried out to understand impact COVID- 19 on Nepalese community dwellers. Methods The...
Abstract Ankylosing spondylitis (AS) is a leading cause of chronic inflammatory low back pain (LBP). A 30-year-old male presented with worsening LBP and difficulty in walking over the last 18 months. Initially, 5 years back, he was diagnosed AS when started to experience morning stiffness (>2 h) lethargy. Symptoms were then manageable medications. For 2 years, it mild. After that, 3 met road traffic accident causing sacral fracture bilateral pubic rami fractures. Even after trauma, his...
Ghosh, Poulami MBBS; Swarnakar, Raktim MD; Soumyadipta Mandal, Pankaj Kumar MD Author Information
Abstract It is commonly recognized that diabetic complications involve increased oxidative stress directly triggered by hyperglycemia. The most important cellular protective systems against such have yet remained unclear. Here we show the selenoprotein thioredoxin reductase 1 (TrxR1), encoded Txnrd1 gene, an essential enzyme for protection. Individually grown knockout ( −/− ) mouse embryonic fibroblasts (MEFs) underwent massive cell death linked to glucose-induced H 2 O production. This and...