A5083888387- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Respiratory and Cough-Related Research
- Air Quality and Health Impacts
- Proteoglycans and glycosaminoglycans research
- Mast cells and histamine
- Public Health and Nutrition
- Medical Imaging and Pathology Studies
- Climate Change and Health Impacts
- Eosinophilic Disorders and Syndromes
- High Altitude and Hypoxia
- Peptidase Inhibition and Analysis
- Hydrogen's biological and therapeutic effects
- Voice and Speech Disorders
- Reconstructive Surgery and Microvascular Techniques
- Respiratory Support and Mechanisms
- Lung Cancer Treatments and Mutations
- Inhalation and Respiratory Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
Lund University
2016-2025
Lung Institute
2014
Mast cells may activate fibroblasts and contribute to remodeling processes in the lung. However, mechanism behind these actions needs be further investigated. Fibroblasts are major regulators of on-going processes. Protease activated receptor 2 (PAR2) expressed by serine proteases, such as mast cell mediator tryptase. The objective this study was investigate effects specifically tryptase on fibroblast migration role PAR2 activation.Human lung (HFL-1) were cultured together with human...
Abstract Remodelling of the extracellular matrix is accomplished by altering balance between macromolecule production and degradation. However, it not well understood how cells new molecules degradation existing ones during tissue remodelling regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic fibroblasts cultured stable isotope labelled amino acids to quantify at a proteome-wide scale. Specific temporal dynamics different matrisome proteins were...
In idiopathic pulmonary fibrosis (IPF) structural properties of the extracellular matrix (ECM) are altered and influence cellular responses through cell-matrix interactions. Scaffolds (decellularized tissue) derived from subpleural healthy IPF lungs were examined regarding biomechanical ECM composition proteins (the matrisome). repopulated with fibroblasts cultured under static stretch heavy isotope amino acids (SILAC), to examine newly synthesized over time. scaffolds characterized by...
Particulate matter (PM) is a major component of ambient air pollution. PM exposure linked to numerous adverse health effects, including chronic lung diseases. Air quality guidelines designed regulate levels are currently based on the mass concentration different particle sizes, independent their origin and chemical composition. The objective this study was assess relative hazardous effects carbonaceous particles (soot), ammonium nitrate, sulfate, copper oxide (CuO), which standard components...
ABSTRACT Background and objective Involvement of pulmonary vascular remodelling is a characteristic sign in COPD . Vascular mediators such as endothelial growth factor ( VEGF ) prostacyclin may regulate fibroblast activity. The was to study the synthesis interactions with transforming TGF )‐β 1 lung fibroblasts from patients healthy control subjects. To further explore autocrine role synthesized on activity, studies were performed human HFL ‐1). Methods Primary distal cultures established...
Abstract Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types cardiovascular diseases. These materials have the potential to maintain functional properties extracellular matrix (ECM), and allow growth remodeling in vivo . The most commonly used methods decellularization are based on chemicals enzymes combinations, which often damage ECM cause cytotoxic effects Mild involving pressurized CO 2 -ethanol (EtOH)-based fluids,...
MicroRNAs have emerged as regulators of smooth muscle cell phenotype with a role in muscle-related disease. Studies shown that miR-143 and miR-145 are the most highly expressed microRNAs cells, controlling differentiation function. The effect miR-143/145 knockout has been established vasculature but not from other organs. Using mice we found maximal contraction induced by either depolarization or phosphatase inhibition was reduced vascular airway maintained urinary bladder. Furthermore,...
Abstract Pulmonary fibrosis is characterized by excessive accumulation of connective tissue, along with activated extracellular matrix ( ECM )‐producing cells, myofibroblasts. The pathological mechanisms are not well known, however serotonin (5‐ HT ) and 5‐ class 2 receptors have been associated fibrosis. aim the present study was to investigate role 2B in fibrosis, using small molecular receptor antagonists EXT 5 9, slightly different affinity. Myofibroblast differentiation [production...
Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes inflammatory responses. Fibroblasts key players remodeling processes major producers of the ECM. The aims this study were to explore effect on cell viability, both ex vivo murine precision cut lung slices (PCLS) vitro human fibroblasts (HFL-1), immunomodulatory responses fibroblasts. PCLS HFL-1 exposed with different sizes, 10 nm 75 nm,...
Introduction: Chronic lung disorders involve pathological alterations in the tissue with hypoxia as a consequence. Hypoxia may influence release of inflammatory mediators and growth factors including vascular endothelial factor (VEGF) prostaglandin (PG)E 2 . The aim this work was to investigate how affects human epithelial cells combination profibrotic stimuli its correlation pathogenesis. Methods: Human bronchial (BEAS-2B) alveolar (hAELVi) were exposed either (1% O ) or normoxia (21%...
Accurate fluid pressure in the fetal lung is critical for its development, especially at beginning of saccular stage when alveolar epithelial type 1 (AT1) and 2 (AT2) cells differentiate from progenitors. Despite our growing understanding role physical forces molecular mechanisms that regulate transduction mechanical stretch to differentiation remain elusive. To simulate distension, we optimized both an ex vivo model with precision cut slices tracheal occlusion. Increased tension showed...
Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate effect of on fibroblast activity migration cells. Lung from IPF patients healthy individuals co-cultured with LAD2 or stimulated proteases tryptase chymase. Human lung cultured cell culture plastic plates decellularized human tissue (scaffolds) create a more physiological milieu by...
Abstract Alveolar epithelial cells (AEC) have been implicated in pathological remodelling. We examined the capacity of AEC to produce extracellular matrix (ECM) and thereby directly contribute towards remodelling chronic lung diseases. Cryopreserved type 2 (AEC2) from healthy lungs obstructive pulmonary disease (COPD) afflicted were cultured decellularized human slices for 13 days. Healthy-derived AEC2 treated with transforming growth factor ß1 (TGF-β1) evaluate plasticity their ECM...
Serotonin (5-hydroxytryptamine) has repeatedly been associated with the development of fibrotic disorders such as pulmonary fibrosis. By blocking binding 5-HT to 5-HT2B receptors receptor antagonists, several pro-fibrotic mechanisms can be inhibited. Bleomycin-induced fibrosis is a model used evaluate pathological and pharmacological interventions. Previously we have shown attenuated in systemic bleomycin-treated mice following treatment two antagonists (EXT5 EXT9). Our aim further identify...
Cigarette smoking is the most common cause of chronic obstructive pulmonary disease (COPD) but more mechanistic studies are needed. smoke extract (CSE) can elicit a strong response in many COPD-related cell types, no have been performed lung fibroblasts. Therefore, we aimed to investigate effect CSE on gene expression fibroblasts from healthy and COPD subjects.Primary fibroblasts, derived six subjects (all current or ex-smokers), were either unstimulated (baseline) stimulated with 30% for 4...
Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts COPD with different responses to chemical stressors compared healthy subjects. Here, aimed investigate differences stress-related gene expressions within lung cells and Bronchoalveolar lavage (BAL) were collected seven 35 Lung derived 19 24 subjects exposed cigarette smoke extract (CSE)....