A5008151390- Melanoma and MAPK Pathways
- Peroxisome Proliferator-Activated Receptors
- Click Chemistry and Applications
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Nanoplatforms for cancer theranostics
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Protein Structure and Dynamics
- Pregnancy and preeclampsia studies
- Coenzyme Q10 studies and effects
- Reproductive System and Pregnancy
- Neuroblastoma Research and Treatments
- Diet, Metabolism, and Disease
- Endoplasmic Reticulum Stress and Disease
- Cancer Research and Treatments
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Polyamine Metabolism and Applications
- Gene expression and cancer classification
- Genomics and Phylogenetic Studies
- Delphi Technique in Research
- Tryptophan and brain disorders
- Autophagy in Disease and Therapy
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2023-2024
Vita-Salute San Raffaele University
2023-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2024
ETH Zurich
2018-2020
Medical University of Vienna
2016-2019
Treatment of BRAFV600E -mutant melanomas with MAPK inhibitors (MAPKi) results in significant tumor regression, but acquired resistance is pervasive. To understand nonmutational mechanisms underlying the adaptation to MAPKi and identify novel vulnerabilities treated MAPKi, we focused on initial response phase during treatment MAPKi.By screening proteins expressed cell surface melanoma cells, identified fatty acid transporter CD36 as most consistently upregulated protein upon short-term MAPKi....
Abstract Succinate dehydrogenase (SDH) is the mitochondrial enzyme converting succinate to fumarate in tricarboxylic acid (TCA) cycle. SDH acts as a tumor suppressor with germline loss-of-function mutations its encoding genes predisposing aggressive familial neuroendocrine and renal cancer syndromes. Lack of activity disrupts TCA cycle, imposes Warburg-like bioenergetic features, commits cells rely on pyruvate carboxylation for anabolic needs. However, spectrum metabolic adaptations enabling...
Abstract Aconitate decarboxylase 1 (ACOD1) is the enzyme synthesizing itaconate, an immuno-regulatory metabolite tuning host-pathogen interactions. Such functions are achieved by affecting metabolic pathways regulating inflammation and microbe survival. However, at whole-body level, roles of itaconate remain largely unresolved. By using multiomics-integrated approaches, here we show that ACOD1 responds to high-fat diet consumption in mice promoting gut microbiota alterations supporting...
During pregnancy, extravillous trophoblasts (EVTs) invade the maternal decidua and remodel local vasculature to establish blood supply for growing fetus. Compromised EVT function has been linked aberrant pregnancy associated with fetal morbidity mortality. However, metabolic features of this invasive trophoblast subtype are largely unknown. Using primary human isolated from first trimester placental tissues, we show that cellular cholesterol homeostasis is differentially regulated in EVTs...
Ketohexokinase (KHK) is the first and rate-limiting enzyme of fructose metabolism. Expression two alternatively spliced KHK isoforms, KHK-A KHK-C, tissue-specific KHK-C predominantly expressed in liver, kidney intestine responsible for fructose-catabolizing function. While isoform choice has been linked to development disorders such as obesity, diabetes, cardiovascular disease cancer, little known about regulation total expression. In present study, we investigated how hypoxic signaling...
Destruxins, secondary metabolites of entomopathogenic fungi, exert a wide variety interesting characteristics ranging from antiviral to anticancer effects. Although their mode action was evaluated previously, the molecular mechanisms resistance development are unknown. Hence, we have established destruxin-resistant sublines HCT116 colon carcinoma cells by selection with most prevalent derivatives, destruxin (dtx)A, dtxB and dtxE. Various cell biological techniques were applied elucidate...
Scavenger receptor class B, type I (SR-BI) is the main for high-density lipoprotein (HDL) and an emerging atheroprotective candidate. A central function of SR-BI delivery HDL-derived cholesterol to liver subsequent excretion into bile. Here, we investigated regulation by unfolded protein response (UPR), adaptive mechanism induced endoplasmic reticulum (ER) stress, which frequently activated in metabolic disorders. We provide evidence that induction acute ER stress well-characterized chemical...
<ns4:p>Next generation sequencing protocols such as RNA-seq have made the genome-wide characterization of transcriptome a crucial part many research projects in biology. Analyses resulting data provide key information on gene expression and certain cases exon or isoform usage. The emergence transcript quantification software Salmon has enabled researchers to efficiently estimate expressions across genome while tremendously reducing necessary computational power. Although overall estimations...
Next generation sequencing protocols such as RNA-seq have made the genome wide characterization of transcriptome a crucial part many research projects in biology. Analyses resulting data provide key information on gene expression and certain cases exon or isoform usage. The emergence transcript quantification software Salmon has enabled researchers to efficiently estimate expressions across while tremendously reducing necessary computational power. Although overall estimations were shown be...
<p>Table S5, related to Figure S5C</p>
<p>Table S3, related to Figure 1 and S1</p>
<p>Contains Supplementary materials and methods, Figures S1-S9, Table S2, S4, Supplemental References Figure S1, related to 1. Cell surface marker screening of A375P cells treated with vehicle (DMSO) or PLX. CD36 is a biomarker the early stage MAPK inhibition in melanoma. S3, induces maintains CD36+ population melanoma 2. MAPKi induce expression genes involved lipid catabolism BRAFV600E melanomas. S5, 3. decreased glycolytic flux BRAF-mutated cells. S6, 4. Phenotypic metabolic...
<p>Table S1 contains antibody details of the LEGENDScreen Human PE Kit from Biolegend</p>
<div>AbstractPurpose:<p>Treatment of <i>BRAF<sup>V600E</sup></i>-mutant melanomas with MAPK inhibitors (MAPKi) results in significant tumor regression, but acquired resistance is pervasive. To understand nonmutational mechanisms underlying the adaptation to MAPKi and identify novel vulnerabilities treated MAPKi, we focused on initial response phase during treatment MAPKi.</p>Experimental Design:<p>By screening proteins expressed cell surface...
<div>AbstractPurpose:<p>Treatment of <i>BRAF<sup>V600E</sup></i>-mutant melanomas with MAPK inhibitors (MAPKi) results in significant tumor regression, but acquired resistance is pervasive. To understand nonmutational mechanisms underlying the adaptation to MAPKi and identify novel vulnerabilities treated MAPKi, we focused on initial response phase during treatment MAPKi.</p>Experimental Design:<p>By screening proteins expressed cell surface...
<p>Table S1 contains antibody details of the LEGENDScreen Human PE Kit from Biolegend</p>
<p>Table S3, related to Figure 1 and S1</p>
<p>Contains Supplementary materials and methods, Figures S1-S9, Table S2, S4, Supplemental References Figure S1, related to 1. Cell surface marker screening of A375P cells treated with vehicle (DMSO) or PLX. CD36 is a biomarker the early stage MAPK inhibition in melanoma. S3, induces maintains CD36+ population melanoma 2. MAPKi induce expression genes involved lipid catabolism BRAFV600E melanomas. S5, 3. decreased glycolytic flux BRAF-mutated cells. S6, 4. Phenotypic metabolic...
<p>Table S5, related to Figure S5C</p>