A5061713973- Advanced Chemical Sensor Technologies
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Analytical Chemistry and Chromatography
- Diet and metabolism studies
- Analytical Methods in Pharmaceuticals
- Pregnancy and preeclampsia studies
- Clinical Nutrition and Gastroenterology
- Antibiotics Pharmacokinetics and Efficacy
- Acute Myeloid Leukemia Research
- Adipose Tissue and Metabolism
- Pharmacological Effects and Toxicity Studies
- Biosimilars and Bioanalytical Methods
- Nicotinic Acetylcholine Receptors Study
- Nutrition and Health in Aging
- Fatty Acid Research and Health
- Chronic Myeloid Leukemia Treatments
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Nutritional Studies and Diet
- Spectroscopy and Chemometric Analyses
- Toxic Organic Pollutants Impact
- Metabolomics and Mass Spectrometry Studies
- Inflammatory Bowel Disease
- Ferroptosis and cancer prognosis
Cancer Research UK
2022-2024
Cancer Research UK Scotland Institute
2022-2024
Ludwig-Maximilians-Universität München
2018-2023
Universidade Federal de Goiás
2017-2023
STCube (United States)
2023
LMU Klinikum
2020-2023
Future University in Egypt
2013-2016
Misr International University
2011
Abstract Background Sarcopenia is one of the most predominant musculoskeletal diseases elderly, defined as age-related progressive and generalized loss muscle mass with a simultaneous reduction in strength and/or function. Using metabolomics, we aimed to examine association between sarcopenia plasma metabolic profile sarcopenic patients, measured using targeted HPLC-MS/MS platform. Methods Plasma samples from 22 (17 men) hip fracture patients undergoing surgery (8 sarcopenic, age 81.4+6.3,...
Abstract Aconitate decarboxylase 1 (ACOD1) is the enzyme synthesizing itaconate, an immuno-regulatory metabolite tuning host-pathogen interactions. Such functions are achieved by affecting metabolic pathways regulating inflammation and microbe survival. However, at whole-body level, roles of itaconate remain largely unresolved. By using multiomics-integrated approaches, here we show that ACOD1 responds to high-fat diet consumption in mice promoting gut microbiota alterations supporting...
Abstract Supporting cell proliferation through nucleotide biosynthesis is an essential requirement for cancer cells. Hence, inhibition of folate-mediated one carbon (1C) metabolism, which required synthesis, has been successfully exploited in anti-cancer therapy. Here, we reveal that mitochondrial folate metabolism upregulated patient-derived leukaemic stem cells (LSCs). We demonstrate 1C impairment de novo purine synthesis a cytostatic effect on chronic myeloid leukaemia (CML) Consequently,...
Abstract In this work, we presented, for the first time, earwax as an alternative forensic specimen detecting 12 neuropsychotic drugs employing liquid chromatography–tandem mass spectrometry in positive and negative ion modes after straightforward extraction with methanol. The method was validated standard curves were established by external calibration correlation coefficients >0.99. All precision, accuracy, matrix effects, recoveries, carryover within acceptable limits; limits of...
Abstract Cancer is the deadliest human disease and development of new diagnosis methods important to increase chances a cure. In this work it was developed method, named here for first time as cerumenogram, using cerumen (earwax) biomatrix diagnosis. Earwax samples collected from cancer patients (cancer group) cancer-free (control were analyzed by Headspace/Gas Chromatography-Mass Spectrometry (HS/GC-MS), following with multivariate analysis steps process raw data generated. total, 158...
Abstract Context Maternal prepregnancy body mass index (BMI) has a strong influence on gestational metabolism, but detailed metabolic alterations are unknown. Objective First, to examine the associations of maternal BMI with early-pregnancy metabolite alterations. Second, identify an profile associated birthweight in women higher that improved prediction compared glucose and lipid concentrations. Design, Setting, Participants Prepregnancy was obtained subgroup 682 Dutch pregnant from...
An innovative method was developed to detect fluoroacetate poisoning in cattle by headspace/gas chromatographic analysis of earwax samples intoxicated cattle. Samples were collected from 2 groups subjected induced intoxication, each group receiving a different dose acetamide (antidote). Monofluoroacetic acid detected concentrations inversely proportional the acetamide. Thus, represents successful approach for detection and monitoring poisoning. • The employs HS/GC-MS detecting exposure It...
Abstract Introduction Adverse exposures in early life may predispose children to cardio-metabolic disease later life. Metabolomics serve as a valuable tool disentangle the metabolic adaptations and mechanisms that potentially underlie these associations. Objectives To describe acquisition, processing structure of metabolomics data available population-based prospective cohort from pregnancy onwards examine relationships between metabolite profiles pregnant women their at birth childhood....
Important metabolic changes occur during transition period of late pregnancy and early lactation to meet increasing energy demands the growing fetus for milk production. The aim this investigation is present an innovative non-invasive tool using ewe earwax sample analysis assess profile in ewes lactation. In work, samples were collected from 28 healthy Brazilian Santa Inês divided into 3 sub-groups: 9 non-pregnant ewes, 6 pregnant last 30 days gestation, 13 lactating ≤ postpartum. Then, a...
High performance liquid chromatographic (HPLC) and spectrophotometric methods developed for the simultaneous determination of Aliskiren (ALK) Hydrochlorothiazide (HCT) combination in bulk powder tablets dosage form. Determination ALK HCT was achieved by separation on Econosphere C-18 column using a mobile phase consisting water (pH 7.5): acetonitrile (50:50) at flow rate 0.5 mL.min?1 UV detection 208 nm. Method validation parameters were found to be acceptable over concentrations range 5-150...
Abstract Background Fetal exposure to bisphenols is associated with altered fetal growth, adverse birth outcomes and childhood cardio-metabolic risk factors. Metabolomics may serve as a tool identify the mechanisms underlying these associations. We examined associations of maternal bisphenol urinary concentrations in pregnancy neonatal metabolite profiles from cord blood. Methods In population-based prospective cohort study among 225 mother–child pairs, A, S F first, second third trimester...
Two simple, accurate and sensitive techniques have been developed validated for the determination of yohimbine hydrochloride in presence its acid, alkaline photo-degradates as stability-indicating studies. First spectrophotometric technique was adopted investigated drug degradation products, by use derivative ratio methods, respectively, while second utilized a high-performance liquid chromatography stability indicating method. The chromatographic separation achieved isocratically using...
(1) Background: Little is known on impacts of ready-to-use therapeutic food (RUTF) treatment lipid metabolism in children with severe acute malnutrition (SAM). (2) Methods: We analyzed glycerophospholipid fatty acids (FA) and polar lipids plasma 41 Pakistani SAM before after 3 months RUTF using gas chromatography flow-injection analysis tandem mass spectrometry, respectively. Statistical was performed univariate, multivariate tests evaluated for the impact age, sex, breastfeeding status,...
Little is known about the metabolic response of pediatric Crohn's disease (CD) patients to partial enteral nutrition (PEN) therapy and impact activity inflammation. We analyzed plasma samples from a nonrandomized controlled intervention study investigating effect on bone health growth throughout one year with untargeted metabolomics using high-performance liquid chromatography (HPLC) coupled high-resolution mass spectrometry (HRMS). Thirty-four paired two time points (baseline 12 months)...