A5090233512- Cancer, Hypoxia, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Ferroptosis and cancer prognosis
- Cancer Research and Treatments
- Sirtuins and Resveratrol in Medicine
- Cancer, Lipids, and Metabolism
- FOXO transcription factor regulation
- Genomics, phytochemicals, and oxidative stress
- Biochemical effects in animals
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Glycosylation and Glycoproteins Research
- Thyroid Disorders and Treatments
- Pharmacogenetics and Drug Metabolism
- Cancer Immunotherapy and Biomarkers
- Lipid metabolism and biosynthesis
- Cancer Cells and Metastasis
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
Cancer Research UK
2019-2024
University of Glasgow
2024
Cancer Research UK Scotland Institute
2019-2023
University Medical Center Groningen
2015-2023
University of Groningen
2015-2023
STCube (United States)
2023
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2013-2018
Paul Ehrlich Institut
1994
Plasmax, a physiological cell culture medium, prevents metabolic artifacts imposed on cancer cells by commonly used media.
The monocarboxylate transporter 8 (MCT8) plays a critical role in mediating the uptake of thyroid hormones (THs) into brain. In patients, inactivating mutations MCT8 gene are associated with severe form psychomotor retardation and abnormal serum TH levels. Here, we evaluate therapeutic potential analog 3,5,3′,5′-tetraiodothyroacetic acid (tetrac) as replacement for T4 brain development. Using COS1 cells transfected deiodinase constructs, could show that tetrac, albeit not being transported...
Article25 June 2015Open Access Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health mice Laura M Zidek Leibniz Institute for Age Research – Fritz Lipmann Institute, Jena, Germany Search more papers by this author Tobias Ackermann European the Biology of Ageing, University Medical Center Groningen, The Netherlands Götz Hartleben Sabrina Eichwald Gertrud Kortman Michael Kiehntopf Department Clinical Chemistry and Laboratory Diagnostics, Hospital Achim Leutz Max...
Cellular metabolism is a tightly controlled process in which the cell adapts fluxes through metabolic pathways response to changes nutrient supply. Among transcription factors that regulate gene expression and thereby cause cellular basic leucine-zipper (bZIP) factor CCAAT/enhancer-binding protein alpha (C/EBPα). Protein lysine acetylation key post-translational modification (PTM) integrates cues with other physiological processes. Here, we show C/EBPα acetylated by acetyl transferase (KAT)...
Abstract The transcription factor C/EBPβ is a master regulator of mammary gland development and tissue remodelling during lactation. CEBPB -mRNA translated into three distinct protein isoforms named C/EBPβ-LAP1, -LAP2 -LIP that are functionally different. smaller isoform LIP lacks the N-terminal transactivation domains considered to act as an inhibitor transactivating LAP1/2 by competitive binding for same DNA recognition sequences. Aberrantly high expression associated with epithelial...
Ageing is associated with physical decline and the development of age-related diseases such as metabolic disorders cancer. Few conditions are known that attenuate adverse effects ageing, including calorie restriction (CR) reduced signalling through mechanistic target rapamycin complex 1 (mTORC1) pathway. Synthesis transcription factor C/EBPβ-LIP stimulated by mTORC1, which critically depends on a short upstream open reading frame (uORF) in Cebpb-mRNA. Here, we describe expression due to...
Glutamine synthetase (GS) activity is conserved from prokaryotes to humans, where the ATP-dependent production of glutamine glutamate and ammonia essential for neurotransmission detoxification. Here, we show that mammalian GS uses methylamine produce a methylated analog, N
Abstract The gamma delta TCR is expressed on 1 to 5% of CD3+ human peripheral blood T lymphocytes. majority cells expresses V 9 paired with 2; this subset strongly responds certain microbial ligands. Other cell subsets unknown Ag specificity expressing different elements are present in and lymphoid tissue. We describe a new anti-human mAb termed 23D12 unusual specificity. As revealed by analysis large number clones transfectants molecularly well-defined TCR, recognized several, but not all,...
The transcription factors LAP1, LAP2 and LIP are derived from the
Cancer cells use glycolysis for generation of metabolic intermediates and ATP needed cell growth proliferation. The transcription factor C/EBPβ-LIP stimulates mitochondrial respiration in cancer cells. We initially observed that high expression makes vulnerable to treatment with the inhibitor 2-deoxyglucose. aim study was uncover involved mechanisms induced sensitivity inhibition.We used genetically engineered lines examine effect -LAP protein isoforms on NADH/NAD+ metabolism mouse embryonic...
Abstract An important part of the beneficial effects calorie restriction (CR) on healthspan and lifespan is mediated through regulation protein synthesis that under control mechanistic target rapamycin complex 1 (mTORC1). As one its activities, mTORC1 stimulates translation into metabolic transcription factor CCAAT/Enhancer Binding Protein β (C/EBPβ) isoform Liver-specific Inhibitory (LIP). Regulation LIP expression strictly depends a re-initiation event requires conserved cis -regulatory...
Abstract Ageing is associated with physical decline and the development of age-related diseases such as metabolic disorders cancer. Few conditions are known that attenuate adverse effects ageing, including calorie restriction (CR) reduced signalling through mechanistic target rapamycin complex 1 (mTORC1) pathway. Synthesis transcription factor C/EBP β ‐LIP stimulated by mTORC1, which critically depends on a short upstream open reading frame (uORF) in β-mRNA. Here we describe expression due...
Abstract The limited availability of therapeutic options for patients with triple-negative breast cancer (TNBC) contributes to the high rate metastatic recurrence and poor prognosis. Ferroptosis is a type cell death caused by iron-dependent lipid peroxidation counteracted antioxidant activity selenoprotein GPX4. Here, we show that TNBC cells secrete an anti-ferroptotic factor in extracellular environment when cultured at densities but are primed ferroptosis forming colonies low density. We...
Cellular metabolism is a tightly controlled process in which the cell adapts fluxes through metabolic pathways response to changes nutrient supply. Among transcription factors that regulate gene expression and thereby cause cellular basic leucine-zipper (bZIP) factor CCAAT/enhancer-binding protein alpha (C/EBPα). Protein lysine acetylation key post-translational modification (PTM) integrates cues with other physiological processes. Here we show C/EBPα acetylated by acetyl transferase (KAT)...
Abstract Oncogene-induced metabolic reprograming supports cell growth and proliferation. Yet, it also links cancer survival to certain pathways nutrients. In order synthesise amino acids nucleotides de novo for proliferation, cells depend on glycolysis, the cytoplasmic oxidation of glucose, which generates necessary intermediates ATP. During NAD+ is used as oxidizing agent thereby reduced into NADH. To ensure high glycolysis rates maintain NADH/NAD+ homeostasis, has be regenerated. The...
Abstract Breast tumorigenesis relies on complex interactions between tumor cells and their surrounding environment, orchestrated by tightly regulated transcriptional networks. While much is known about the signaling pathways affecting breast cancer cell growth apoptosis, programs driving these responses are less understood. C/EBPβ a transcription factor essential for proper cellular proliferation, differentiation, tissue function, metabolism, immune response, deregulated in cancer. its...