A5036614502- Telomeres, Telomerase, and Senescence
- Erythrocyte Function and Pathophysiology
- Mesenchymal stem cell research
- Caveolin-1 and cellular processes
- Estrogen and related hormone effects
- Endometriosis Research and Treatment
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Extracellular vesicles in disease
- Gestational Trophoblastic Disease Studies
- Reproductive Biology and Fertility
- Lymphatic System and Diseases
- Uterine Myomas and Treatments
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Prenatal Screening and Diagnostics
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- IL-33, ST2, and ILC Pathways
- Lipid metabolism and disorders
- Blood disorders and treatments
- Nuclear Structure and Function
- Connective tissue disorders research
Johannes Kepler University of Linz
2021-2025
University of Applied Sciences Upper Austria
2018-2023
Medical University of Vienna
2006-2018
Max Perutz Labs
2006-2017
Parker Hannifin (United States)
2009
Miele (Germany)
2009
Vienna Biocenter
2002-2006
University of Vienna
2002
IL-33, the most recently discovered member of IL-1 superfamily and ligand for transmembrane form ST2 (ST2L), has been linked to several human pathologies including rheumatoid arthritis, asthma, cardiovascular disease. Deregulated levels soluble ST2, natural IL-33 inhibitor, have reported in sera preeclamptic patients. However, role during healthy pregnancy remains elusive. In current study, was detected culture supernatants placental decidual macrophages, identifying them as a major source...
Genome amplification and cellular senescence are commonly associated with pathological processes. While physiological roles for polyploidization have been described in mouse development, controversy exists over their significance humans. Here, we describe tetraploidization as phenomena of normal human placenta development. During pregnancy, placental extravillous trophoblasts (EVTs) invade the pregnant endometrium, termed decidua, to establish an adapted microenvironment required developing...
High-resolution imaging is essential for analysis of the steps and way stations cargo transport in
Stomatin is an ancient, widely expressed, oligomeric, monotopic membrane protein that associated with cholesterol-rich membranes/lipid rafts. It part of the SPFH superfamily including stomatin-like proteins, prohibitins, flotillin/reggie bacterial HflK/C proteins and erlins. Biochemical features such as palmitoylation, oligomerization, hydrophobic "hairpin" structure show similarity to caveolins other integral scaffolding proteins. Recent analyses conserved PHB/SPFH domain revealed amino...
Abstract Osteogenesis imperfecta (OI) is a genetically and clinically heterogeneous disorder characterized by bone fragility reduced mass generally caused defects in type I collagen structure or proteins interacting with processing. We identified homozygous missense mutation SEC16B child vertebral fractures, leg bowing, short stature, muscular hypotonia, densitometric histomorphometric features keeping OI distinct ultrastructural features. In line the putative function of as regulator...
The cytoplasmically oriented monotopic integral membrane protein stomatin forms high-order oligomers and associates with lipid rafts. To characterize the domains that are involved in oligomerization detergent-resistant (DRM) association, we expressed truncation point mutants of analyzed their size buoyancy by ultracentrifugation methods. A small C-terminal region is largely hydrophobic, Ser-Thr-Ile-Val-Phe-Pro-Leu-Pro-Ile (residues 264-272), proved to be crucial for oligomerization, whereas...
While therapeutic cell transplantations using progenitor cells are increasingly evolving towards phase I and II clinical trials chemically defined culture is established, standardization in biobanking still the stage of infancy. In this study, EU FP6-funded CRYSTAL (CRYo-banking Stem for human Therapeutic AppLication) consortium aimed to validate novel Standard Operating Procedures (SOPs) perform xeno-free cryopreservation reduce amount potentially toxic cryoprotectant additive (CPA)...
Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance stromal vascular fraction (SVF) cell population in lipedema, we performed thorough characterization subcutaneous tissue, SVF isolated thereof and sorted populations endothelial cells (EC), pericytes cultured adipose-derived stromal/stem (ASC) early-stage lipedema patients. We employed histological gene expression analysis investigated barrier by immunofluorescence permeability vitro....
The human stomatin-like protein-1 (SLP-1) is a membrane protein with characteristic bipartite structure containing stomatin domain and sterol carrier protein-2 (SCP-2) domain. This suggests role for SLP-1 in sterol/lipid transfer transport. Because has not been investigated, we first studied the molecular cell biological characteristics of expressed protein. We show here that localizes to late endosomal compartment, like stomatin. Unlike stomatin, does localize plasma membrane....
Despite intense efforts, the exact phenotype of epidermal Langerhans cell (LC) precursors during human ontogeny has not been determined yet. These elusive are believed to migrate into embryonic skin and express primitive surface markers, including CD36, but typical LC markers such as CD1a, CD1c CD207. The aim this study was further characterize in epidermis compare it with that LCs healthy adult skin. We found leukocytes first trimester negative for CD34 heterogeneous regard expression CD1c,...
Physiologically, following myocardial infarction (MI), retinoid levels elevate locally in the infarcted area. Whereas therapeutic systemic application of retinoids was shown to reduce progression ventricular dilatation and onset heart failure, role acute physiologically increased zone is unknown date. To reveal local MI central aim this study. Using human cell culture co-culture models for hypoxia as well various assays systems, lentivirus-based transgene expression, silico molecular docking...
Over-expression of fluorescently-labeled markers for extracellular vesicles is frequently used to visualize vesicle up-take and transport. EVs that are labeled by over-expression show considerable heterogeneity regarding the number fluorophores on single particles, which could potentially bias tracking studies in favor more strongly-labeled particles. To avoid potential artefacts caused over-expression, we developed a genome editing approach fluorescent labeling marker CD63 with green...
Extracellular vesicles (EVs) play a key role in cell–cell communication and thus have great potential to be utilized as therapeutic agents diagnostic tools. In this study, we implemented single-molecule microscopy techniques toolbox for comprehensive characterization well measurement of the cellular uptake HEK293T cell-derived EVs (eGFP-labeled) HeLa cells. A combination fluorescence atomic force revealed fraction 68% fluorescently labeled with an average size ∼45 nm. Two-color analysis...
Abstract Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance stromal vascular fraction (SVF) cell population in lipedema, we performed thorough characterization subcutaneous tissue, SVF isolated thereof and sorted populations endothelial cells (EC), pericytes cultured adipose-derived stromal/stem (ASC) early-stage lipedema patients. We employed histological gene expression analysis investigated barrier by immunofluorescence permeability...
Zoledronic acid (ZA) has antiresorptive effects and protects from bone metastasis in women with early breast cancer. In addition, postmenopausal endocrine responsive cancer ZA prolongs DFS. The exact mechanism is still unclear. We have therefore investigated the effect of increasing concentrations cell lines absence or presence estradiol to mimic hormonal environment vitro.Using assays for proliferation (EZ4U, BrdU) death (Annexin/PI), we analyzed dose-dependent antiproliferative...
RTN1 is an endoplasmic reticulum-associated protein that was initially identified in neuronal tissues. Here we show the main isoform RTN1A a marker for dendritic cells. In skin, HLA-DR+CD1ahighCD207+CD11cweak Langerhans cells were only epidermis, and HLA-DR+CD11c+ dermis, expressing this protein. RTN1A+ also found gingiva, trachea, tonsil, thymus, peripheral blood. During differentiation of MUTZ-3 into cells, expression mRNA preceded established cell markers CD1a CD207, partially...
The corpus luteum (CL) is under control of gonadotrophic hormones and produces progesterone, which necessary for endometrial receptivity. Recent studies have shown that progesterone its metabolites are involved in cell proliferation apoptosis cancer cells. Here weanalyzed the role metabolites on luteinized granulosa cells (LGC) by FACS analysis quantitative Real-Time PCR. We detected mRNA metabolizing genes SRD5A1, AKR1C1, AKR1C2 LGC. stimulation LGC with or did not show any effect...