A5030459032- Terahertz technology and applications
- Advanced Fluorescence Microscopy Techniques
- Nanofabrication and Lithography Techniques
- Microwave and Dielectric Measurement Techniques
- Nonlinear Optical Materials Studies
- Photonic Crystals and Applications
- Mechanical and Optical Resonators
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Advanced Fiber Optic Sensors
- Photochromic and Fluorescence Chemistry
- Platelet Disorders and Treatments
- Thermal Radiation and Cooling Technologies
- Advancements in Transdermal Drug Delivery
- Photonic and Optical Devices
- Optical Network Technologies
- Advanced biosensing and bioanalysis techniques
- Acoustic Wave Resonator Technologies
- Ocular Surface and Contact Lens
- Carbon Nanotubes in Composites
- Semiconductor materials and interfaces
- Thermal properties of materials
University of Applied Sciences Upper Austria
2021-2023
Johannes Kepler University of Linz
2016-2020
Universidad Carlos III de Madrid
2014-2016
University of Kerala
2010
Vellore Institute of Technology University
2010
Abstract Biomimetics is the interdisciplinary scientific field focused on study and imitation of biological systems, with aim solving complex technological problems. In this paper, we present a new bio-inspired design for microneedles (MNs) MN arrays, intended rapidly coating MNs drug/vaccine. The biomimetic approach consists in ornamenting lateral sides pyramidal structures inspired by external scent efferent systems some European true bugs, which facilitate directional liquid transport. To...
We employed noncontact terahertz time-domain spectroscopy to investigate and compare the electrical/optical properties of nonaligned single-walled carbon nanotube multiwalled thin films. Using Drude–Lorentz model together with Maxwell Garnett effective medium theory, we determined electrical conductivities from extracted data differential complex analysis in frequency range 0.1 2 THz. The results demonstrate that conductivity isotropic film is almost two times larger compared due increased...
Abstract We present localization with stimulated emission depletion (LocSTED) microscopy, a combination of STED and single-molecule microscopy (SMLM). use the simplest form microscope that is cost effective synchronization free, comprising continuous wave (CW) lasers for both excitation depletion. By utilizing reversible blinking fluorophores, single molecules Alexa 555 are localized down to ~5 nm. Imaging fluorescently labeled proteins attached nanoanchors structured by lithography shows...
We present the software platform 2CALM that allows for a comparative analysis of 3D localisation microscopy data representing protein distributions in two biological samples. The in-depth statistical reveals differences between samples at nanoscopic level using parameters such as cluster-density and -curvature. An automatic classification system combines multiplex multi-level approaches into one comprehensive parameter similarity testing compared demonstrated importance 2CALM, comparing CD41...
Over-expression of fluorescently-labeled markers for extracellular vesicles is frequently used to visualize vesicle up-take and transport. EVs that are labeled by over-expression show considerable heterogeneity regarding the number fluorophores on single particles, which could potentially bias tracking studies in favor more strongly-labeled particles. To avoid potential artefacts caused over-expression, we developed a genome editing approach fluorescent labeling marker CD63 with green...
Extracellular vesicles (EVs) play a key role in cell–cell communication and thus have great potential to be utilized as therapeutic agents diagnostic tools. In this study, we implemented single-molecule microscopy techniques toolbox for comprehensive characterization well measurement of the cellular uptake HEK293T cell-derived EVs (eGFP-labeled) HeLa cells. A combination fluorescence atomic force revealed fraction 68% fluorescently labeled with an average size ∼45 nm. Two-color analysis...
We explore the optical and electrical characteristics of monolayer graphene by using pulsed optoelectronic terahertz time-domain spectroscopy in frequency range 325–500 GHz based on fast direct measurements phase amplitude. also show that these parameters can, however, be measured with higher resolution a free space continuous wave measurement technique associated vector network analyzer offers good dynamic range. All scattering (both magnitude phase) are simultaneously. The...
A vector network analyser is used to study the electrical properties of multi‐walled carbon nanotube (MWCNT) thin films deposited on a fused quartz substrate in sub‐terahertz (THz) frequency ranges 220–325 GHz (WR3.4) and 325–500 (WR2.2). The experiment performed free space. complex permittivity MWCNT extracted using Nicholson‐Ross‐Weir method. refractive index conductivity are then determined from permittivity. method validated by comparison with values obtained THz time‐domain spectroscopy.
Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down single molecule level. We now combined STED with laser-assisted adsorption by photobleaching (LAPAP) optical and selective attachment of proteins subdiffractional structures. In turn, used imaging fluorescently tagged streptavidin reveal binding STED-lithographically patterned acrylate structures via LAPAP. Protein...
Abstract Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due diffraction. We show that, similar stimulated emission depletion (STED) microscopy, depleting beam can effectively suppress LAPAP and hence apt locally control in order write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 glass substrates incubate with Alexa 555 labeled...
Multiwalled carbon nanotube (MWCNT) thin films deposited on fused quartz substrates are characterized by continuous wave subterahertz free space technique using a vector network analyzer (VNA). The scattering (S) parameters measured VNA in the frequency range of 220–325 GHz (G-band) and 325–500 (Y-band). complex refractive index is extracted with help Nicholson–Ross–Weir method. transmission absorption also studied. offers good spectral resolution dynamic 60–70 dB around 500 GHz....
A free space, non-destructive, vectorial continuous wave terahertz analysis method is employed to study the material properties of single-walled and multi-walled carbon nanotube thin films deposited on substrates fused quartz silicon. The electrical optical have been investigated using a vector network analyzer, in frequency range 220–325 GHz (WR 3.4) 325–500 2.2). Nicolson–Ross–Weir used extract parameters. refractive index, absorption coefficient, complex conductivity are extracted studied.
Polymer micro- and nanostructures with various geometries can be written onto a transparent substrate by the technique of two-photon polymerization Ti-sapphire femtosecond-laser, which is focused into liquid acrylate based resin containing photo-initiator. The microstructures employed either as three-dimensional tissue scaffold adherent cells seeded or are used topological substrates for wetting experiments. Due to confinement and/or mechanical interaction scaffold, skin derived stimulated...
Photonic crystal fibers have attracted increasing interest over the past few years because of their ability to provide manipulation in optical properties light. High birefringence can be easily achieved PCFS based on design flexibility and large index contrast. Amongst several designs high exceeding 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">−3</sup> has been shown. Birefringence PCF further improved by employing elliptical air holes...