Bulk gold shows photoluminescence (PL) with a negligible quantum yield of ∼10–10, which can be increased by orders magnitude in the case nanoparticles. This bears huge potential to use noble metal nanoparticles as fluorescent and unbleachable stains bioimaging or for optical data storage. Commonly, enhancement PL is attributed nanoparticle plasmons, specifically enhancements scattering absorption cross sections. Tuning shape geometry nanostructures (e.g., via reducing distance between two...

Surface reactive nanostructures were fabricated using stimulated emission depletion (STED) lithography. The functionalization of the was realized by copolymerization a bifunctional metal oxo cluster in presence triacrylate monomer. Ligands surface cross-link to monomer during lithographic process, whereas unreacted mercapto functionalized ligands are transferred polymer and remain after formation nanostructure. efficiency dependence loading investigated full STED effect observed with...

High-resolution imaging is essential for analysis of the steps and way stations cargo transport in

Interest in mesenchymal stem cell derived extracellular vesicles (MSC-EVs) as therapeutic agents has dramatically increased over the last decade. Current approaches to characterization and quality control of EV-based therapeutics include particle tracking techniques, Western blotting, advanced cytometry, but standardized methods are lacking. In this study, we established verified quartz crystal microbalance (QCM) highly sensitive label-free immunosensing technique for characterizing...

The fabrication of two- and three-dimensional scaffolds mimicking the extracellular matrix providing cell stimulation is high importance in biology material science. We show two new, biocompatible polymers, which can be 3D structured

We demonstrate that the newly designed amino acid phosphordiamidate-resins (APdA), containing vinyl reactive groups for polymerization, can be utilized to fabricate sub-100 nm features through 3D multiphoton lithography. have quantitatively analyzed feature size, Young's modulus, and functionalization of nanostructures using atomic force single-molecule fluorescence microscopy. Our results indicate polymer backbone, composed either valine or alanine, imparts hydrophobic properties monomer,...

An electromagnetic wave impinging on a gold nanosponge coherently excites many hot-spots inside the nanosponge, yielding polarization-dependent scattering spectrum. In contrast, hole, recombining with an electron, can locally excite plasmonic only within horizon given by lifetime of localized plasmons and speed carrying information that plasmon has been created. This is about 57 nm, decreasing increasing size nanosponge. Consequently, photoluminescence from large nanosponges appears unpolarized.

Here, we report that hybrid multilayered plasmonic nanostars can be universally used as feedback agents for coherent random lasing in polar or nonpolar solutions containing gain material. We show silver-enhancement of gold reduces the pumping threshold substantially both a typical dye (R6G) and fluorescent polymer (MEH-PPV). Further, reveal intensity lasers based on silver-enhanced are not influenced by silica coating, contrast to nanostar-based lasers, where silica-coated support only...

Recently, 2D/3D direct laser writing has attracted increased attention due to its broad applications ranging from biomedical engineering aerospace. 3D nanolithography of water-soluble protein-based scaffolds have been envisioned provide a variety tunable properties. In this paper, we present functional photoresist with mechanical properties that is suitable for multiphoton lithography (MPL). Through the use methacrylated streptavidin or bovine serum albumin in combination polyethylene glycol...

Bonding individual metallic nanoparticles at small separation distances to let them form dimers and making available in large quantities is a key requirement for various applications that wish exploit the tremendous enhancement of electromagnetic fields plasmonic junctions. Although progress has been witnessed past concerning fabrication mediated by rigid molecular linkers, exact bonding mechanism remains unclear. Here, we describe linker molecule demonstrate its feasibility achieve made...

Utilizing Bragg surface plasmon polaritons (SPPs) on metal nanostructures for the use in optical devices has been intensively investigated recent years. Here, we demonstrate integration of nanostructured electrodes into an ITO-free thin film bulk heterojunction organic solar cell, by direct fabrication a nanoimprinted substrate. The device shows interesting and electrical behavior, depending angle polarization incidence side excitation. Remarkably, through top electrode, dependency linear...

Over-expression of fluorescently-labeled markers for extracellular vesicles is frequently used to visualize vesicle up-take and transport. EVs that are labeled by over-expression show considerable heterogeneity regarding the number fluorophores on single particles, which could potentially bias tracking studies in favor more strongly-labeled particles. To avoid potential artefacts caused over-expression, we developed a genome editing approach fluorescent labeling marker CD63 with green...

Extracellular vesicles (EVs) play a key role in cell–cell communication and thus have great potential to be utilized as therapeutic agents diagnostic tools. In this study, we implemented single-molecule microscopy techniques toolbox for comprehensive characterization well measurement of the cellular uptake HEK293T cell-derived EVs (eGFP-labeled) HeLa cells. A combination fluorescence atomic force revealed fraction 68% fluorescently labeled with an average size ∼45 nm. Two-color analysis...

Stimulated emission depletion (STED) has been used to break the diffraction limit in fluorescence microscopy. Inspired by this success, similar methods were reduce structure size three-dimensional, subdiffractional optical lithography. So far, only a very limited number of radical polymerization starters proved be suitable for STED-inspired In contribution, we introduce starter Michler's ethyl ketone (MEK), which not so far contrast commonly 7-diethylamino-3-thenoylcoumarin (DETC),...

We present newly developed buffer systems that significantly improve the efficiency of a photochemically induced surface modification at single molecule level. Buffers with paramagnetic cations and radical oxygen promoting species facilitate laser-assisted protein adsorption by photobleaching (LAPAP) fluorescently labelled oligonucleotides or biotin onto multi-photon-lithography-structured 2D 3D acrylate scaffolds. Single fluorescence microscopy has been used to quantify photopainting...

If two metal nanoparticles are ultimately approached, a tunneling current prevents both an infinite redshift of the bonding dipolar plasmon and increase electric field in hot spot between nanoparticles. We argue that Coulomb blockade suppresses sustains even for sub-nanometer approach up to moderate fields. Only stronger optical fields, is lifted charge transfer formed. Numerical simulations show such scenarios well reach with manageable nanoparticle dimensions, at room temperature....

The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in tissue culture dish or directly with tissues (roots, parts of plants, and cell tissues). Here, we present transwell system that allows on monolayer. We introduce measurement window insert membrane, which provides direct access cells to media upper lower compartment cell-to-cell contact coculture....

We present a new method for generating protein-based tissue engineering 3D scaffolds with adaptable mechanical properties. Multiphoton lithography allowed fabrication of 2/3D structures feature sizes below 200 nm lateral and 600 axial Young's moduli down to 40 kPa. The viability mechanotransduction mesenchymal stem cells within the were shown by imaging cellular actin cytoskeleton vinculin protein density via direct stochastic optical reconstruction microscopy. Surface functionalization...

<title>Abstract</title> The use of two wavelengths to activate different photoreactions in a resin system has recently attracted much attention the scientific community. Here, wavelength orthogonal photochemistry was used spatially control curing kinetics thiol-ene photopolymerization reaction. In investigated system, radical is activated by type II photoinitiator at 450 nm, while light 365 nm photorelease base, resulting an inhibition antagonistic nature these demonstrated laser writing and...

The plasmonic tip's emission can be controlled in liquids depending on the wetting condition and refractive index of liquids.

Summary form only given. Here we report on promising strategies for photon management in organic optolectronic devices. Multilayered hybrid plasmonic nanostars are used to boost the performance of light-emitting diodes (OLEDs). We designed and synthesized silver-enhanced gold coated with a very thin silica layer (~10 nm), so that hot-spots at tips remain easily accessible, while emission quenching is reduced. The spectral position plasmon resonances tuned specific regions optimal overlap...

Gold nanosponges are gold/air percolated nanoparticles with three dimensionally intertwined gold and air filaments of sub-20 nm diameter. The internal structure the nanosponges, i.e. size can be tuned by varying fabrication parameters. exhibit multiple scattering resonances in visible to NIR spectral range. Using dark field optical spectroscopy scanning electron microscopy, it is feasible determine their plasmonic response relate morphology a specific single nanosponge. We found that...

Summary form only given. Compared to bulk gold, nanoparticles show significantly higher quantum yield of photoluminescence (PL) from d-band hole recombination [1]. It makes them attractive for cell imaging and biosensing. The underlying mechanism the PL is a subject intensive debates, although there are numerous reports on single-photon multi-photon-induced plasmonic particles nanoparticle systems. Here, we experimentally numerically that hot-spot intensity between two gold bipyramids...