A5002687657- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Hemophilia Treatment and Research
- Mast cells and histamine
- Hemostasis and retained surgical items
- Microbial metabolism and enzyme function
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Veterinary Pharmacology and Anesthesia
- Blood Coagulation and Thrombosis Mechanisms
- Complement system in diseases
- Food Allergy and Anaphylaxis Research
- Anesthesia and Sedative Agents
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nitric Oxide and Endothelin Effects
- Platelet Disorders and Treatments
- Animal testing and alternatives
Medical University of Vienna
2020-2021
University of Veterinary Medicine Vienna
2018
Baxter (Austria)
2010-2013
Intravenous (IV) administration in mice is predominantly performed via the lateral tail veins. The technique requires adequate training before it can be used safely and routinely. A novel anaesthesia induction chamber has been developed to simplify treatment facilitate IV injection mice, particularly for untrained personnel. We have assessed benefits of refining isoflurane-anaesthetized direct comparison with common restrainer method on conscious animals. body weight, nesting behaviour...
To measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine horseradish peroxidase (HRP)/hydrogen peroxide (H2O2) coupling must be used. The use of material should avoided and HRP/H2O2 is compromised by antioxidants.Condensation ortho-aminobenzaldehyde (oABA) delta-1-pyrroline delta-1-piperideine, the autocyclization products DAO-oxidized natural substrates cadaverine, generates new quinazoline fluorophores...
Background: Excessive plasma histamine concentrations cause symptoms in mast cell activation syndrome, mastocytosis, or anaphylaxis. Anti-histamines are often insufficiently efficacious. Human diamine oxidase (hDAO) can rapidly degrade and therefore represents a promising new treatment strategy for conditions with pathological concentrations. Methods: Positively charged amino acids of the heparin-binding motif hDAO were replaced polar serine threonine residues. Binding to heparin heparan...
Human diamine oxidase (hDAO) rapidly inactivates histamine by deamination. No pharmacokinetic data are available to better understand its potential as a new therapeutic modality for diseases with excess local and systemic histamine, like anaphylaxis, urticaria or mastocytosis. After intravenous administration of recombinant hDAO rats mice, more than 90% the dose disappeared from plasma pool within 10 min. DAO did not only bind various endothelial epithelial cell lines in vitro, but was also...
Abstract Elevated plasma and tissues histamine concentrations can cause severe symptoms in mast cell activation syndrome, mastocytosis or anaphylaxis. Endogenous recombinant human diamine oxidase (rhDAO) rapidly completely degrade histamine, administration of rhDAO represents a promising new treatment approach for diseases with excess release from activated cells. We recently generated heparin-binding motif mutants considerably increased vivo half-lives rodents compared the cleared wildtype...