A5090362608- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Synthesis and Biological Evaluation
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- Quinazolinone synthesis and applications
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Biosimilars and Bioanalytical Methods
- Immune cells in cancer
- Chemokine receptors and signaling
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Calcium signaling and nucleotide metabolism
- Hepatitis B Virus Studies
- Research on Leishmaniasis Studies
- COVID-19 and healthcare impacts
- vaccines and immunoinformatics approaches
- Extracellular vesicles in disease
Navarre Institute of Health Research
2015-2024
Universidad de Navarra
2006-2024
Centro de Investigación Biomédica en Red de Cáncer
2018-2024
Clinica Universidad de Navarra
2016-2024
Universitat de Barcelona
2024
Hospital Clínic de Barcelona
2024
Centro de Investigación Biomédica en Red
2021
CIC biomaGUNE
2014-2018
Chartered Institute of Management Accountants
2008-2013
Gene Therapy Laboratory
2007-2013
Knowledge about the mechanism of action (MoA) monoclonal antibodies (mAb) is required to understand which patients with multiple myeloma (MM) benefit most from a given mAb, alone or in combination therapy. Although there considerable research daratumumab, knowledge other anti-CD38 mAbs remains scarce.We performed comprehensive analysis MoA isatuximab.Isatuximab induces internalization CD38 but not its significant release MM cell surface. In addition, we uncovered an association between...
Hepatitis C virus (HCV) chronic infection is characterized by low or undetectable cellular immune responses against HCV antigens. Some studies have suggested that proteins manipulate the system suppressing specific antiviral T-cell immunity. We previously reported expression of core and E1 (CE1) in dendritic cells (DC) impairs their ability to prime T vitro. show here immunization mice with immature DC transduced an adenovirus encoding antigens (AdCE1) induced lower CD4(+)- CD8(+)-T-cell...
Natural killer (NK) cells exhibit a polarized phenotype with increased cytotoxicity and decreased interferon gamma (IFN–γ) production in chronic hepatitis C virus (HCV) infection. Here, we asked whether this is caused by type I (IFN)–induced expression phosphorylation levels of signal transducer activator transcription (STAT) molecules NK it affects the response refractoriness to IFN–α–based therapy HCV. STAT1 were significantly higher patients HCV infection than uninfected controls....
Development of vaccines to prevent and treat emerging new pathogens re-emerging infections cancer remains a major challenge. An attractive approach is build the vaccine upon biocompatible NP that simultaneously acts as accurate delivery vehicle radiotracer for PET/SPECT imaging ultrasensitive quantitative in vivo target tissues/organs. Success developing these nanovaccines will depend part on having "correct" size accommodating suitably displaying antigen and/or adjuvants (e.g., TLR...
Abstract Purpose: B-cell maturation antigen (BCMA)-chimeric receptor T-cells (CART) improve results obtained with conventional therapy in the treatment of relapsed/refractory multiple myeloma. However, high demand and expensive costs associated CART might prove unsustainable for health systems. Academic CARTs could potentially overcome these issues. Moreover, response biomarkers resistance mechanisms need to be identified addressed efficacy patient selection. Here, we present clinical...
Large-scale immune monitoring is becoming routinely used in clinical trials to identify determinants of treatment responsiveness, particularly immunotherapies. Flow cytometry remains one the most versatile and high throughput approaches for single-cell analysis; however, manual interpretation multidimensional data poses a challenge when attempting capture full cellular diversity provide reproducible results. We present FlowCT, semi-automated workspace empowered analyze large sets. It...
There is major current interest in harnessing the immune system against cancer and developing drugs that provide complementary killing mechanisms. Although recent advent of nanoparticle‐based drug delivery systems has improved efficacy platinum for chemotherapy, one fundamental paradigms their design use evading surveillance by to enhance anticancer efficacy. However, new studies are showing chemotherapy can profit from actively targeting stimulation suitably functionalized nanomaterials...
Abstract Tumor recognition by T cells is essential for antitumor immunity. A comprehensive characterization of cell diversity may be key to understanding the success immunomodulatory drugs and failure PD-1 blockade in tumors such as multiple myeloma (MM). Here, we use single-cell RNA receptor sequencing characterize bone marrow from healthy adults ( n = 4) patients with precursor 8) full-blown MM 10). Large clones expressed immune checkpoints, suggesting a potentially dysfunctional...
Information on the immunopathobiology of coronavirus disease 2019 (COVID-19) is rapidly increasing; however, there remains a need to identify immune features predictive fatal outcome. This large-scale study characterized responses severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection using multidimensional flow cytometry, with aim identifying high-risk biomarkers. Holistic and unbiased analyses 17 cell-types were conducted 1,075 peripheral blood samples obtained from 868...
Abstract: Injection of dendritic cells (DCs) presenting viral proteins constitutes a promising approach to stimulate T cell immunity against hepatitis C virus (HCV). Here we describe strategy enhance antigen loading and immunostimulatory functions DCs useful in the preparation therapeutic vaccines. Incubation murine with CFm40L, an adapter molecule containing coxsackie-adenovirus receptor fused ecto-domain CD40L-induced DC maturation, produced high amounts interleukin-12 up-regulation...
Daratumumab is an anti-CD38 fully human IgG1 mAb approved for multiple myeloma treatment. One of the proposed mechanisms action induction antibody-dependent cellular cytotoxicity (ADCC) mediated by NK cells. cells acquire surface CD137 expression in presence solid-phase-attached daratumumab and when encountering a daratumumab-coated CD38+ tumor cell line. In this setting, addition agonist anti-CD137 urelumab enhances NK-cell activation increasing CD25 IFNɣ production. However, vitro ADCC not...
7544 Background: The activity and safety ofARI0002h, an academic autologous CAR T-cell product with a humanized single chain variable fragment targeting BCMA has been reported in pilot multicenter clinical trial (CARTBCMA-HCB-01) treating 30 patients (pts) RRMM (NCT04309981) (Oliver-Caldés, Lancet Onc 2023). Here, we describe results of the final cohort 60 pts longer follow-up. Methods: Patients aged 18-75 years old were eligible if they had measurable disease, refractory to last line...
Chronic infection by hepatitis C virus (HCV) is characterized the absence of efficient antiviral T-cell responses. Thus, vaccination strategies to induce strong anti-HCV responses are paramount importance for prophylactic and therapeutic purposes. Dendritic cells (DCs) most potent antigen presenting cells; therefore, immunization with these loaded viral antigens offers a new approach induction immunity. Here we show that DCs transfected an adenovirus encoding non-structural 3 protein, from...