A5080073728- Radiopharmaceutical Chemistry and Applications
- Nanoparticle-Based Drug Delivery
- Medical Imaging Techniques and Applications
- Boron Compounds in Chemistry
- Neuroscience and Neuropharmacology Research
- Nanoplatforms for cancer theranostics
- Chemical Reactions and Isotopes
- Alzheimer's disease research and treatments
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Advanced MRI Techniques and Applications
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Micro and Nano Robotics
- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Radiation Therapy and Dosimetry
- Lanthanide and Transition Metal Complexes
- Drug Transport and Resistance Mechanisms
- Vagus Nerve Stimulation Research
- Click Chemistry and Applications
- Receptor Mechanisms and Signaling
- Sulfur Compounds in Biology
CIC biomaGUNE
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2024
Universidad Nacional Arturo Jauretche
2024
Hospital El Cruce
2024
Boeing (Spain)
2020
Digital Research Alliance of Canada
2020
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2008-2020
Ayuntamiento de San Sebastián
2015-2020
Diputación Foral de Gipuzkoa
2020
California State University, San Bernardino
2019
Enzyme-powered nanomotors are an exciting technology for biomedical applications due to their ability navigate within biological environments using endogenous fuels. However, limited studies into collective behavior and demonstrations of tracking enzyme in vivo have hindered progress toward clinical translation. Here, we report the swarming urease-powered its positron emission tomography (PET), both vitro vivo. For that, mesoporous silica nanoparticles containing urease enzymes gold were...
Micro/nanomotors are useful tools for several biomedical applications, including targeted drug delivery and minimally invasive microsurgeries. However, major challenges such as in vivo imaging need to be addressed before they can safely applied on a living body. Here, we show that positron emission tomography (PET), molecular technique widely used medical imaging, also track large population of tubular Au/PEDOT/Pt micromotors. Chemisorption an iodine isotope onto the micromotor's Au surface...
Abstract Bladder cancer treatment via intravesical drug administration achieves reasonable survival rates but suffers from low therapeutic efficacy. To address the latter, self-propelled nanoparticles or nanobots have been proposed, taking advantage of their enhanced diffusion and mixing capabilities in urine when compared with conventional drugs passive nanoparticles. However, translational treating bladder are underexplored. Here, we tested radiolabelled mesoporous silica-based...
Murine models of Plasmodium falciparum malaria may become crucial tools in drug discovery. Here we show that non-myelodepleted NOD-scid IL2Rgamma(null) mice engrafted with human erythrocytes support an infectious burden up to tenfold higher than supported by beta2microglobulin(null) mice. The new model was validated for discovery and used assess the therapeutic efficacy 4-pyridones, selective inhibitors P. cytochrome bc1.
The extraordinary small size of NPs makes them difficult to detect and quantify once distributed in a material or biological system. We present simple straightforward method for the direct proton beam activation synthetic commercially available aluminum oxide (Al2O3 NPs) via 16O(p,α)13N nuclear reaction order assess their fate using positron emission tomography (PET). radiolabeling does not alter surface structural properties as demonstrated by TEM, DLS, ζ-potential measurements....
New tools and techniques to improve brain visualization assess drug permeability across the blood-brain barrier (BBB) are critically needed. Positron emission tomography (PET) is a highly sensitive, noninvasive technique that allows evaluation of BBB under normal disease-state conditions. In this work, we have developed synthesis novel water-soluble biocompatible glucose-coated gold nanoparticles (GNPs) carrying BBB-permeable neuropeptides chelator positron emitter (68)Ga as PET reporter for...
Triple-negative breast cancer (TNBC) diagnosis remains challenging without expressing critical receptors. Cancer cell membrane (CCm) coating has been extensively studied for targeted diagnostics due to attractive features such as good biocompatibility and homotypic tumor-targeting. However, the present study found that widely used CCm approaches, extrusion, were not applicable functionalizing irregularly shaped nanoparticles (NPs), porous silicon (PSi). To tackle this challenge, we proposed...
[ 11 C]PK11195 is used in positron emission tomography (PET) studies for imaging brain inflammation vivo as it binds to the peripheral-type benzodiazepine receptor (PBR) expressed by reactive glia and macrophages. However, features of cellular reaction required induce a positive signal are not well characterized. We performed PET autoradiography rats after transient focal cerebral ischemia. determined 3 H]PK11195 binding PBR expression tissue examined lesion with several markers. standard...
Microglia, the resident immune cells of central nervous system, responds to brain disarrangements by becoming activated contend with damage. Here we show that expression P2X4 receptors is upregulated in inflammatory foci and microglia spinal cord rats experimental autoimmune encephalomyelitis (EAE) as well optic nerve multiple sclerosis patients. To study role microgliosis, LPS vitro vivo . We observed receptor activity was increased LPS‐activated assessed patch‐clamp recordings. In...
Development of vaccines to prevent and treat emerging new pathogens re-emerging infections cancer remains a major challenge. An attractive approach is build the vaccine upon biocompatible NP that simultaneously acts as accurate delivery vehicle radiotracer for PET/SPECT imaging ultrasensitive quantitative in vivo target tissues/organs. Success developing these nanovaccines will depend part on having "correct" size accommodating suitably displaying antigen and/or adjuvants (e.g., TLR...
Abstract A synthetic antimicrobial peptide was identified as a possible candidate for the development of new antibacterial drug. The peptide, SET-M33L, showed MIC 90 below 1.5 μM and 3 Pseudomonas aeruginosa Klebsiella pneumoniae , respectively. In in vivo models P. infections, its pegylated form (SET-M33L-PEG) enabled survival percentage 60–80% sepsis lung infections when injected twice i.v. at 5 mg/Kg, completely healed skin administered topically. Plasma clearance different kinetics...
PEG coated magnetic nanocarriers avoid the reticuloendothelial system, and show an MRI contrast in kidneys. The results are supported by SPECT, gamma-counting, TEM histology.
Abstract: A novel nanocarrier system was designed and developed with key components uniquely structured at the nanoscale for early cancer diagnosis treatment. In order to perform magnetic resonance imaging, hydrophilic superparamagnetic maghemite nanoparticles (NPs) were synthesized coated a lipophilic organic ligand. Next, they entrapped into polymeric NPs made of biodegradable poly(lactic-co-glycolic acid) linked polyethylene glycol. addition, resulting have been conjugated on their...
The development of tools for the early diagnosis pancreatic adenocarcinoma is an urgent need in order to increase treatment success rate and reduce patient mortality. Here, we present a modular nanosystem platform integrating soft nanoparticles with targeting peptide active imaging agent diagnostics. Biocompatible single-chain polymer (SCPNs) based on poly(methacrylic acid) were prepared functionalized somatostatin analogue PTR86 as moiety, since receptors are overexpressed cancer. gamma...
Background: Boron Neutron Capture Therapy (BNCT) is a binary approach to cancer therapy that requires accumulation of boron atoms preferentially in tumour cells. This can be achieved by using nanoparticles as carriers and taking advantage the enhanced permeability retention (EPR) effect. Here, we present preparation characterization size shape-tuned gold NPs (AuNPs) stabilised with polyethylene glycol (PEG) functionalized boron-rich anion cobalt bis(dicarbollide), commonly known COSAN. The...
In vivo Positron Emission Tomography (PET) imaging of the cystine-glutamate antiporter (system xc -) activity with [ 18 F]FSPG is meant to be an attractive tool for diagnosis and therapy evaluation brain diseases.However, role system -in cerebral ischemia its involvement in inflammatory reaction has been scarcely explored.In this work, we report longitudinal investigation neuroinflammatory process following transient middle artery occlusion (MCAO) rats using PET translocator protein (TSPO)...
While mesoporous silica nanoparticles (MSNs) are extensively studied as high-potential drug delivery platforms, the successful clinical translation of these nanocarriers strongly depends on their biodistribution, biodegradation, and elimination patterns in vivo. Here, a novel method is reported to follow vivo degradation MSNs by tracking radioactive label embedded structure. Core-shell (NPs) with dense core shell labeled low quantities positron emitter 89 Zr, either or shell. In emission...
Aging | doi:10.18632/aging.100651. Arantza Infante, Andrea Gago, Garbiñe Ruiz de Eguino, Teresa Calvo-Fernández, Vanessa Gómez-Vallejo, Jordi Llop, Karin Schlangen, Ane Fullaondo, Ana M. Aransay, Abraham Martín, Clara I. Rodríguez
Studies of the uptake, biological fate, and toxicity several metal oxide nanoparticles (NPs), such as Al 2 O 3 , TiO CeO 2‐x ZnO NPs undertaken in European Project “Health Impact Engineered Metal Oxide Nanoparticles: Response, Bioimaging Distribution at Cellular Body Level” are reviewed here. radiolabeled by direct proton bombardment commercially available or enriched during synthesis with 18 to generate F after‐proton bombardment. Size, degree aggregation, zeta potential studied presence...
Triple negative breast cancer (TNBC) is the deadliest form of and its successful treatment critically depends on early diagnosis therapy. The multi-compartment protein p32 overexpressed present at cell surfaces in a variety tumors, including TNBC, specifically malignant cells endothelial cells, macrophages localized hypoxic areas tumor. Herein we used polyethylene glycol-polycaprolactone polymersomes that were affinity targeted with p32-binding tumor penetrating peptide LinTT1 (AKRGARSTA)...
A labelling method for the covalent attachment of radioiodine to boron-rich 8-I-cobaltabisdicarbollide (I-COSAN) and a bi-functional (iodine PEG) COSAN derivative, [3,3'-Co(8-I-1,2-C2B9H10)(8'-(OCH2CH2)2COOC6H5-1',2'-C2B9H10)], is reported. Biodistribution studies in rodents using dissection/gamma counting vivo nuclear imaging have been performed. The general strategy reported here can be applied future derivatives bearing wide range functionalities.
PET imaging of nicotinic acetylcholine receptors (nAChRs) could become an effective tool for the diagnosis and therapy evaluation neurologic diseases. Despite this, role nAChRs α4β2 after brain diseases such as cerebral ischemia its involvement in inflammatory reaction is still largely unknown. To investigate we performed parallel vivo magnetic resonance (MRI) positron emission tomography (PET) with 2[(18)F]-fluoro-A85380 [(11)C]PK11195 at 1, 3, 7, 14, 21, 28 d middle artery occlusion (MCAO)...
Sildenafil (Viagra) is a selective inhibitor of phosphodiesterase type 5 (PDE5), which degrades cyclic guanosine monophosphate to the linear nucleotide. acutely used in erectile dysfunction and chronically pulmonary hypertension. Evidence last decade shows that sildenafil may have potential as therapeutic option for Alzheimer's disease or other neurodegenerative disorders. The purpose this work was explore whether crosses blood-brain barrier. Pharmacokinetic properties rodents were...