A5071603429- Nanoparticle-Based Drug Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Electrostatics and Colloid Interactions
- Protein Interaction Studies and Fluorescence Analysis
- Polymer Surface Interaction Studies
- Molecular Junctions and Nanostructures
- Advanced Proteomics Techniques and Applications
- Proteins in Food Systems
- Protein purification and stability
- Advanced Nanomaterials in Catalysis
- Algal biology and biofuel production
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Carbon dioxide utilization in catalysis
- Chemical Synthesis and Analysis
- Metal-Organic Frameworks: Synthesis and Applications
- Dendrimers and Hyperbranched Polymers
- Nanoparticles: synthesis and applications
- Nanocluster Synthesis and Applications
- Nanomaterials for catalytic reactions
- Field-Flow Fractionation Techniques
- Echinoderm biology and ecology
- Seaweed-derived Bioactive Compounds
CIC biomaGUNE
2017-2025
Boeing (Spain)
2025
Digital Research Alliance of Canada
2025
Ayuntamiento de San Sebastián
2021
CIC bioGUNE
2019
Weatherford College
2019
Norwich Research Park
2013-2017
Quadram Institute
2015-2017
University of East Anglia
2013-2017
Nanocomposites comprising hydrogels and plasmonic nanoparticles are attractive materials for tissue engineering, bioimaging, biosensing. These usually fabricated by adding colloidal to the uncured polymer mixture thus require time-consuming presynthesis, purification, ligand-exchange steps. Herein, we introduce approaches rapid synthesis of gold nanostars (AuNSt)
Triple negative breast cancer (TNBC) is the deadliest form of and its successful treatment critically depends on early diagnosis therapy. The multi-compartment protein p32 overexpressed present at cell surfaces in a variety tumors, including TNBC, specifically malignant cells endothelial cells, macrophages localized hypoxic areas tumor. Herein we used polyethylene glycol-polycaprolactone polymersomes that were affinity targeted with p32-binding tumor penetrating peptide LinTT1 (AKRGARSTA)...
Multidimensional kinetic analysis of immobilized enzymes is essential to understand the enzyme functionality at interface with solid materials. However, spatiotemporal characterization heterogeneous biocatalysts on a microscopic level and under operando conditions has been rarely approached. As case study, we selected self-sufficient where His-tagged cofactor-dependent (dehydrogenases, transaminases, oxidases) are co-immobilized their corresponding phosphorylated cofactors [nicotinamide...
λ-type carrageenan (λ-CAR) polysaccharides remain overlooked in the preparation of medical nanoparticles (NP) due to their unsuitable rheological properties and undesired biological effects, although they can also offer advantageous properties. To overcome these obstacles, oligosaccharide derivatives (λ-COS) have been successfully applied synthesis stable NP incorporating both, ferrite cores divalent manganese (Mn2+) releasable ions. The acute pro-inflammatory behavior anticoagulant activity...
Nanoparticles (NPs) in contact with biological fluids are generally coated environmental proteins, forming a stronger layer of proteins around the NP surface called hard corona. Protein corona complexes provide identity NPs and their isolation characterization essential to understand vitro vivo behaviour. Here we present one-step methodology recover from complex media stable non-aggregated form without affecting structure or composition This method allows be separated containing particulates...
Atherosclerosis is a complex disease that can lead to life-threatening events, such as myocardial infarction and ischemic stroke. Despite the severity of this disease, diagnosing plaque vulnerability remains challenging due lack effective diagnostic tools. Conventional protocols specificity fail predict type atherosclerotic lesion risk rupture. To address issue, technologies are emerging, noninvasive medical imaging with customized nanotechnological solutions. Modulating biological...
The incidence and mortality of cancer demand more innovative approaches combination therapies to increase treatment efficacy decrease off-target side effects. We describe a boron-rich nanoparticle composite with potential applications in both boron neutron capture therapy (BNCT) photothermal (PTT). Our strategy is based on gold nanorods (AuNRs) stabilized polyethylene glycol functionalized the water-soluble complex cobalt bis(dicarbollide) ([3,3'-Co(1,2-C2B9H11)2]-), commonly known as COSAN....
“Click” dendrimer-Pd NPs as peroxidase enzyme mimics of H<sub>2</sub>O<sub>2</sub> sensing using <italic>o</italic>-phenylenediamine oxidation by to 2,3-diaminophenazine.
COVID-19, caused by the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), originated a global health crisis, causing over million casualties and altering human daily life all world. This pandemic emergency revealed limitations of current diagnostic tests, highlighting urgency to develop faster, more precise sensitive sensors. Graphene field effect transistors (GFET) are analytical platforms that enclose these requirements. However, design robust GFET is not straightforward...
Functionalization of nanoparticles with specific ligands is helpful to control diagnostic and therapeutic responses such as protein adsorption, cell targeting, circulation. Precision delivery critically depends on a fundamental understanding the interplay between surface chemistry, ligand dynamics, interaction biochemical environment. Due limited atomic-scale insights into structure dynamics nanoparticle-bound from experiments, relationships grafting density chemistry observable properties...
The Cu-glutathione (GSH) redox system, essential in biology, is designed here as a supramacromolecular assembly which the tetrahedral 18e Cu(I) center loses thiol ligand upon adsorption onto ZIF-8, shown by EXAFS and DFT calculation, to generate very robust 16e planar trigonal single-atom catalyst. Synergy between revealed catalytic experiments affords CO
Fluorescence correlation spectroscopy allows the of state aggregation <italic>in vitro</italic> with intracellular fate gold NPs for different antifouling coatings.
We present a computational and experimental study on the folding aggregation in solutions of multiple protein mixtures at different concentrations. show how each component is capable maintaining its folded state densities greater than one which they would precipitate single-species solutions. demonstrate generality our observation over many proteins using computer simulations fully characterizing cross-aggregation phase diagram all mixtures. Dynamic light scattering experiments were...
Self-sufficient heterogeneous biocatalysts (ssHB) are promising candidates for implementing cofactor-dependent enzymes in chemical biomanufacturing. Most strategies coimmobilizing cofactors with dehydrogenases on porous agarose microbeads involve the use of cationic polymers (i.e., polyethylenimine, PEI) that interact electrostatically phosphate groups their corresponding phosphorylated cofactors. Although latter is a powerful and versatile approach, ionic bonds disrupted biotransformations...
Abstract Cells exhibit diverse structural formations such as biofilms and spores, enabling them to acquire novel functionalities. Many of these structures display biomacromolecules, including enzymes, tethered cell walls support various extracellular processes. Alternatively, encapsulating single cells with polymer coatings offers a strategy that circumvents the need for genetic engineering while imparting artificial functionalities cells. Here, universal method is presented gram‐negative...
With the rise in production of nanoparticles (NPs) for an ever-increasing number applications, there is urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring determine overall toxicity index.We observed that, after exposure range NPs, Xenopus phenotypic showed strong correlation cell based vitro assays. Magnetite-cored negative and Xenopus, were further...
In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to working mechanism, surface functionalisation immobilisation receptors are pivotal ensure proper functioning devices. Herein, we present controlled covalent strategy based on molecular design electrochemical triggering, which provide monolayer-like micro-GFET arrays retaining...
Biological fate and toxicity of nanoparticles (NPs) are connected to the interaction between NPs protein corona (PC) spontaneously forming around in biological matrixes. PC is a dynamic entity that confers identity NPs. In this work, fluorescence cross-correlation spectroscopy (FCCS) used study impact specific interactions NP surface proteins on intracellular PC. The stability formed glucosamide-functionalized Au-NPs from ConcanavalinA (ConA) or Bovine Serum Albumin (BSA) characterized by...
Negatively charged poly(N-isopropylacrylamide-co-methacrylic acid) (P(NIPAm-co-MAA)) microgels undergo size changes in response to temperature and pH. Complexation of these with positively polyelectrolytes can greatly affect their physical properties capacity for encapsulating active molecules. Here we study the interaction between a model polyelectrolyte, poly allylamine hydrochloride (PAH), different molecular weights. Experiments were conducted at temperatures below above lower critical...