A5024719839- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Lanthanide and Transition Metal Complexes
- DNA and Nucleic Acid Chemistry
- Supramolecular Self-Assembly in Materials
- Advanced MRI Techniques and Applications
- Surfactants and Colloidal Systems
- Crystallography and molecular interactions
- Nanoplatforms for cancer theranostics
- Characterization and Applications of Magnetic Nanoparticles
- Nanocluster Synthesis and Applications
- Nanoparticles: synthesis and applications
- Polymer Surface Interaction Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Electron Spin Resonance Studies
- Spectroscopy and Quantum Chemical Studies
- Medical Imaging Techniques and Applications
- Electrostatics and Colloid Interactions
- Advanced Nanomaterials in Catalysis
- Fluorine in Organic Chemistry
- Advanced Drug Delivery Systems
Politecnico di Milano
2016-2025
European Institute of Oncology
2024
Mylan (Switzerland)
2015-2020
Tecnologia Energia Ambiente Materiali (Italy)
2014-2020
Milano University Press
2015-2019
Material (Belgium)
2017-2019
Curculio Institute
2015-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
IRCCS Ospedale San Raffaele
2019
Vita-Salute San Raffaele University
2019
It is now clearly emerging that besides size and shape, the other primary defining element of nanoscale objects in biological media their long-lived protein ("hard") corona. This corona may be expressed as a durable, stabilizing coating bare surface nanoparticle (NP) monomers, or it reflected different subpopulations particle assemblies, each presenting durable coating. Using approach concepts physical chemistry, we relate studies on composition at plasma concentrations with structural data...
What the biological cell, organ, or barrier actually "sees" when interacting with a nanoparticle dispersed in medium likely matters more than bare material properties of particle itself. Typically surface is covered by several biomolecules, including select group proteins drawn from medium. Here, we apply different methodologies, time-resolved manner, to follow lifetime such biomolecular "coronas" both situ and isolated excess plasma. We find that particle−biomolecule complexes can be...
Protein adsorption to nanoparticles (NPs) is a key prerequisite understand NP-cell interactions. While the layer thickness of protein corona has been well characterized in many cases, absolute number bound proteins and their exchange dynamics body fluids difficult assess. Here we measure molecules adsorbed sulfonate (PSOSO(3)H) carboxyl-(PSCOOH) polystyrene NPs using fluorescence correlation spectroscopy. We find that fraction falls onto single, universal curve, if plotted as function molar...
Superparamagnetic iron oxide nanoparticles (SPIONs) have proved their use in many biomedical applications, such as drug delivery, hyperthermia, and MRI (magnetic resonance imaging) contrast agents. Due to instability fluids, several surface coatings been used both stabilize tune the properties of these (NPs) according applications. These will strongly modify influence interaction with environment proteins a relevant biological medium clear impact on function. It is well-accepted that protein...
To control liposomes fate and transport upon contact with biofluids, it is essential to consider several parameters affecting the synthetic biological identity of liposomes, as well liposome-protein corona (PC) aspects. As a powerful tool in this data mining adventure, quantitative structure-activity relationship (QSAR) approach used correlate physicochemical properties their PC fingerprints multiple quantified responses. In present study, between cellular interactions set structurally...
19F-MRI offers unique opportunities to image diseases and track cells therapeutic agents in vivo. Herein we report a superfluorinated molecular probe, herein called PERFECTA, possessing excellent cellular compatibility, whose spectral properties, relaxation times, sensitivity are promising for vivo applications. The molecule, which bears 36 equivalent 19F atoms shows single intense resonance peak, is easily synthesized via simple one-step reaction formulated water with high stability using...
Nanomedicine aims to find novel solutions for urgent biomedical needs. Despite this, one of the most challenging hurdles that nanomedicine faces is successfully target therapeutic nanoparticles cells interest in vivo. As any biomaterials, once vivo, can interact with plasma biomolecules, forming new entities which name protein coronas (PCs) have been coined. The PC influence vivo biological fate a nanoparticle. Thus guaranteeing desired function an engineered nanomaterial it crucial dissect...
In this work we have studied the effect of a low-frequency alternating magnetic field (LF-AMF) on permeability magnetoliposomes, i.e. liposomes including nanoparticles within their water pool. Large unilamellar loaded with cobalt ferrite (CoFe2O4) been prepared and characterized. Structural characterization liposomal dispersion has performed by dynamic light scattering (DLS). The enhancement liposome upon exposure to LF-AMF measured as self-quenching decrease fluorescent hydrophilic molecule...
It is now well established that the surface of nanoparticles (NPs) in a biological environment immediately modified by adsorption biomolecules with formation protein corona and it also accepted corona, rather than original nanoparticle surface, defines new identity. Consequently, methodology to effectively study interaction between nanomaterials encountered within an organism key objective nanoscience for understanding impact nanoparticle-protein interactions on response vitro vivo. Here, we...
The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on permeability and release properties large (LUVs) giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). shell allows high loading NPs in lipid without modifying their properties. increase LUVs upon exposure to LF-AMF has been evaluated as fluorescence self-quenching carboxyfluorescein (CF) entrapped inside liposome aqueous pool. Liposome leakage monitored...
Ultrasound is the most commonly used clinical imaging modality. However, in applications requiring cell-labeling, large size and short active lifetime of ultrasound contrast agents limit their longitudinal use. Here, 100 nm radius, clinically applicable, polymeric nanoparticles containing a liquid perfluorocarbon, which enhance during repeated over course at least 48 h, are described. The perfluorocarbon enables monitoring with quantitative 19F magnetic resonance imaging, making these...
KEYWORDS: Protein coronananotechnologybio-nano interfacescolloidal stabilitymucus penetrationintestinal absorptionoral drug delivery
Designing short antimicrobial peptides and tuning their interaction with bacterial barriers.
Nanoparticle biomolecular corona leads the interactions with cognate proteins on arrays of thousands immobilised human proteins.
One of the main hurdles in nanomedicine is low stability drug–nanocarrier complexes as well drug delivery efficiency region-of-interest. Here, we describe use film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable vivo and efficiently encapsulate hydrophobic molecules. film prevents massive release encapsulated drug, which, instead, activated by selective SP...