A5040610833- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Lipid Membrane Structure and Behavior
- Graphene and Nanomaterials Applications
- DNA and Nucleic Acid Chemistry
- Characterization and Applications of Magnetic Nanoparticles
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Polymer Surface Interaction Studies
- Pancreatic and Hepatic Oncology Research
- Nanopore and Nanochannel Transport Studies
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Gold and Silver Nanoparticles Synthesis and Applications
- Immunotherapy and Immune Responses
- Protein Interaction Studies and Fluorescence Analysis
- Advanced Proteomics Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
- Electrostatics and Colloid Interactions
- Surfactants and Colloidal Systems
- Graphene research and applications
- Microplastics and Plastic Pollution
- Immune Cell Function and Interaction
Sapienza University of Rome
2016-2025
Yahoo (United Kingdom)
2023
Università Campus Bio-Medico
2018-2021
Istituto Pasteur
2019-2020
Istituti Fisioterapici Ospitalieri
2016-2017
Italian Institute of Technology
2014
Austrian Academy of Sciences
2005-2010
Università di Camerino
2007-2010
Marche Polytechnic University
2006
University of Salerno
2005
When nanoparticles (NPs) enter a physiological environment, medium components compete for binding to the NP surface leading formation of rich protein shell known as "protein corona". Unfortunately, opsonins are also adsorbed. These proteins immediately recognized by phagocyte system with rapid clearance NPs from bloodstream. Polyethyleneglycol (PEG) coating (PEGylation) is most efficient anti-opsonization strategy. Linear chains PEG, grafted onto surface, able create steric hindrance,...
When nanoparticles (NPs) enter a biological fluid (e.g., human plasma (HP)), proteins and other biomolecules adsorb on the surface leading to formation of rich protein shell, referred as "protein corona". This corona is dynamic in nature its composition varies over time due continuous association dissociation events. Understanding evolution time-scales particle's lifetime blood fundamental predict fate vivo. In this study, we used lipid NPs, cationic...
Abstract In vivo liposomes, like other types of nanoparticles, acquire a totally new ‘biological identity’ due to the formation biomolecular coating known as protein corona that depends on and modifies liposomes’ synthetic identity. The liposome–protein is dynamic interface regulates interaction liposomes with physiological environment. Here we show biological identity clearly linked their sequestration from peripheral blood mononuclear cells (PBMCs) healthy donors ultimately leads removal...
Abstract Lipofectamine reagents are widely accepted as “gold-standard” for the safe delivery of exogenous DNA or RNA into cells. Despite this, a satisfactory mechanism-based explanation their superior efficacy has remained mostly elusive thus far. Here we apply straightforward combination live cell imaging, single-particle tracking microscopy and quantitative transfection-efficiency assays on cells to unveil intracellular trafficking mechanism Lipofectamine/DNA complexes. We find that...
The possibility to combine Low Intensity UltraSound (LIUS) and Nanoparticles (NP) could represent a promising strategy for drugs delivery in tumors difficult treat overcoming resistance therapies. On one side the NP can carry that specifically target on other LIUS facilitate direct tumor cells. In this study, we investigated whether Very (VLIUS), at intensities lower than 120 mW/cm2, might constitute novel improve Thus, order verify efficacy of modality terms increase selective uptake...
A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers vivo, can be used for targeting. Here, we show that nanoparticles acquire a selective targeting capability with corona adsorbed on surface. We demonstrate lipid particles made of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and DNA, upon interaction human plasma components, spontaneously become coated vitronectin promotes efficient uptake cancer cells expressing high levels...
To control liposomes fate and transport upon contact with biofluids, it is essential to consider several parameters affecting the synthetic biological identity of liposomes, as well liposome-protein corona (PC) aspects. As a powerful tool in this data mining adventure, quantitative structure-activity relationship (QSAR) approach used correlate physicochemical properties their PC fingerprints multiple quantified responses. In present study, between cellular interactions set structurally...
The structure and composition of the liposome–protein corona are dependent on incubation protocol (<italic>i.e.</italic> dynamic <italic>vs.</italic> static incubation).
When injected in a biological milieu, nanomaterial rapidly adsorbs biomolecules forming biomolecular corona. The corona changes the interfacial composition of giving it identity that determines physiological response. Characterization structure and has received increasing attention mostly due to its detrimental impact on nanomaterial's metabolism vivo. It is generally accepted an opsonin-enriched promotes immune system recognition rapid clearance from circulation. Here we applied dynamic...
Exosomes are a class of nanovesicles formed and released through the late endosomal compartment represent an important mode intercellular communication. The ability anticancer chemotherapy to enhance immunogenic potential malignant cells mainly relies on establishment cell death (ICD) release damage-associated molecular patterns (DAMPs). Here, we investigated whether genotoxic stress could promote exosomes from multiple myeloma (MM) studied immunomodulatory properties they exert NK cells,...
As soon as nanomaterials, such nanoparticles (NPs), are injected into a physiological environment rich coating of biomolecules known the "protein corona" rapidly covers them. This protein dress is main factor, which affects interaction NPs with living systems. While relationship between NP features and biomolecule corona has been extensively investigated, whether how changes in affect NP–protein remains under-investigated. one most important steps translating results animal models to clinic....
Nowadays, liposomes are the most successful drug delivery systems with a dozen products available in clinic. Grafting poly-(ethylene glycol) (PEG) onto liposome surface prevents protein binding thus prolonging blood circulation, while synthetic modification of terminal PEG molecule ligands (e.g. monoclonal antibodies and peptides) should promote selective accumulation tumor region respect to healthy tissues. However, despite big efforts, advances have not outgrown development stage just few...
Protein corona sensor array technology identifies diseases through specific proteomics pattern recognition.
Pancreatic cancer is a very aggressive malignancy that often diagnosed in the advanced stages, with implication long-term survivors are extremely rare. Thus, developing new methods for early detection of pancreatic an urgent task current research. To date, nanotechnology offers unprecedented opportunities therapeutics and diagnosis. The aim this study development diagnostic technology based on exploitation nano-bio-interactions between nanoparticles blood samples. In study, samples from 20...
For several decades, surface grafted polyethylene glycol (PEG) has been a go-to strategy for preserving the synthetic identity of liposomes in physiological milieu and preventing clearance by immune cells. However, limited clinical translation PEGylated is mainly due to protein corona formation subsequent modification liposomes' identity, which affects their interactions with cells blood residency. Here we exploit electric charge DNA generate unPEGylated liposome/DNA complexes that, upon...
A new generation of an FFP2 (Filtering Face Piece type 2) smart face mask is achieved by integrating broadband hybrid nanomaterials and a self-assembled optical metasurface. The multifunctional shows simultaneously white light-assisted on-demand disinfection properties versatile biosensing capabilities. These are powerful combination light thermoplasmonic responsive nanomaterials, which provide excellent photo-thermal properties, metasurface-based colorimetric biosensors, with very low limit...
The concept that the effective unit of interest in cell-nanomaterial interaction is particle and its corona associated proteins emerging. Here we investigate compositional evolution protein 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) DOTAP/DNA lipoplexes over a wide range plasma concentrations (2.5-80%). composition hard quite stable, but CLs does evolve considerably. We show made both low-affinity competitive-binding whose relative abundance changes with...