A5080526227- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Optical Sensing Technologies
- Particle Detector Development and Performance
- Astrophysics and Cosmic Phenomena
- Advanced biosensing and bioanalysis techniques
- Optical Imaging and Spectroscopy Techniques
- Machine Learning in Materials Science
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Particle physics theoretical and experimental studies
- Dark Matter and Cosmic Phenomena
- Photoacoustic and Ultrasonic Imaging
- Recycling and Waste Management Techniques
- Microplastics and Plastic Pollution
- RNA Research and Splicing
- Radiation Therapy and Dosimetry
- Advanced Biosensing Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Phagocytosis and Immune Regulation
- Metabolomics and Mass Spectrometry Studies
- Molecular Sensors and Ion Detection
- Graphene and Nanomaterials Applications
- CCD and CMOS Imaging Sensors
- Integrated Circuits and Semiconductor Failure Analysis
Laboratori Guglielmo Marconi (Italy)
2020-2024
Italian Institute of Technology
2021-2023
Radiolabs
2022
University of Genoa
2019-2021
Irvine University
2021
University of California, Irvine
2016-2021
Ingegneria dei Sistemi (Italy)
2021
University of Florence
2019
Istituto Nazionale di Fisica Nucleare, Sezione di Firenze
2017-2019
Fluor (United States)
2016
Abstract Lipofectamine reagents are widely accepted as “gold-standard” for the safe delivery of exogenous DNA or RNA into cells. Despite this, a satisfactory mechanism-based explanation their superior efficacy has remained mostly elusive thus far. Here we apply straightforward combination live cell imaging, single-particle tracking microscopy and quantitative transfection-efficiency assays on cells to unveil intracellular trafficking mechanism Lipofectamine/DNA complexes. We find that...
Abstract Muography is an imaging technique based on the measurement of absorption profiles for muons as they pass through rocks and earth. Muons are produced in interactions high-energy cosmic rays Earth’s atmosphere. The conceptually similar to usual X-ray radiography, but with extended capabilities investigating over much larger thicknesses matter thanks penetrating power muons. Over centuries a complex system cavities has been excavated yellow tuff Mt. Echia, site earliest settlement city...
We packaged condensed DNA/protamine particles in multicomponent envelope-type nanoparticle systems (MENS) combining different molar fractions of the cationic lipids 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 3β-[N-(N,N-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) zwitterionic dioleoylphosphocholine (DOPC) dioleoylphosphatidylethanolamine (DOPE). Dynamic light scattering (DLS) microelectrophoresis allowed us to identify lipid/DNA charge ratio at which MENS are small sized...
Abstract Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access an entirely set of information. Together with the blooming SP LSM techniques and development tailored there growing need for (i) DAQ systems capable handling high-throughput high-resolution photon information generated by these (ii) incorporating protocols in existing fluorescence LSMs. We developed open-source, low-cost, multi-channel...
Fluorescence lifetime analysis (FLA) has become essential for non-invasive, real-time of a variegate realm compounds and materials in scientific industrial fields. Here we present compact versatile device designed to apply FLA the screening healthcare nanoformulations which contain luminescent active principle. In case study here: Doxil enables decode supramolecular organization stability principle within nanoformulation rapid (seconds), cost-effective manner. Contrary currently used methods...
Given the good performances in terms of geometrical acceptance and energy resolution, calorimeters are best suited detectors to measure high cosmic rays directly space. However, order exploit this potential, design must be carefully optimized take into account all limitations related space missions, due mainly mass experimental apparatus. CaloCube is a three years R&D project, approved financed by INFN 2014, aiming optimize space-borne calorimeter use cubic, homogeneous isotropic geometry....
Fluorescence lifetime-based technology has experienced a rapid growth during the last decade, becoming unique tool for performing real-time, non-invasive analysis in large variety of scientific fields, from life-sciences to medical surgery and agrifood. Its potential aroused interest industry technological shift detection estimation fluorescence lifetime is around corner. Nevertheless, devices capable implementing state-of-the-art that are compact, customizable affordable, still incredibly...
Ribonucleoprotein (RNP) condensates partition RNA and protein into multiple liquid phases. The multiphasic feature of condensate-enriched components creates experimental challenges for distinguishing membraneless condensate functions from the surrounding dilute phase. We combined fluorescence lifetime imaging microscopy (FLIM) with phasor plot filtering segmentation to resolve Condensate-specific lifetimes were used track protein–protein interactions by measuring FLIM–Förster resonance...
Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to the introduction of new asynchronous read-out single-photon (SP) array detectors. These detectors give access an entirely set information typically lost in conventional fluorescence LSM, thus triggering imaging/spectroscopy paradigm – so-called singlephoton LSM (SP-LSM). The revolution’s outcomes are, from one side, blooming SP-LSM techniques and tailored SP detectors; other need for data-acquisition (DAQ)...
The presence of plastic fragments in aquatic environments, particularly those micro- and nano-scale, has become a significant global concern. However, current detection methods are limited revealing the such small particles liquid samples. In this study, we propose use fluorescence lifetime analysis system as tool for nanoplastics water. This approach relies on inherent endogenous materials involves collecting single photons emitted by when exposed to pulsed laser beam. Our successfully...