A5016068729- Classical Studies and Legal History
- Advanced Fluorescence Microscopy Techniques
- Pancreatic function and diabetes
- Antibiotics Pharmacokinetics and Efficacy
- Microbial Inactivation Methods
- Comparative International Legal Studies
- Lipid Membrane Structure and Behavior
- Ancient Mediterranean Archaeology and History
- Advanced biosensing and bioanalysis techniques
- Reproductive System and Pregnancy
- Pharmacological Effects of Natural Compounds
- Neuroendocrine regulation and behavior
- Pharmacological Effects and Toxicity Studies
- Pain Mechanisms and Treatments
- Pharmaceutical studies and practices
- Hormonal and reproductive studies
- Membrane-based Ion Separation Techniques
- Diabetes and associated disorders
- Ion channel regulation and function
- Chemical Reaction Mechanisms
- Network Packet Processing and Optimization
- Photoacoustic and Ultrasonic Imaging
- Research on Leishmaniasis Studies
- Neuroscience and Neuropharmacology Research
- Malaria Research and Control
Scuola Normale Superiore
2021-2025
National Enterprise for NanoScience and NanoTechnology
2023
University College Dublin
2019-2021
Hacettepe University
2021
Sapienza University of Rome
2018-2019
University of Pavia
1976-1997
University of Bologna
1994
Escuela Superior Politécnica del Chimborazo
1989
It is widely accepted that the pH of insulin granules acidic, and its active regulation during granule maturation plays a role in process secretion by β-cells. Yet, calibrated measurement absolute with organelle specificity still lacking. To tackle this issue, we use genetically encoded E1GFP reporter inserted into C-peptide proinsulin expressed Insulinoma 1E cells. Following verification correct targeting granules, phasor-based Fluorescence Lifetime Imaging Microscopy (FLIM) applied to...
The effects of components aqueous licorice root extract (LE) on the pharmacokinetics glycyrrhizin (G) and glycyrrhetic acid (GA) were investigated in rats humans. aim this work was to define role G toxicity. In procedure, GA detected biological fluids by means recently improved HPLC methods. Significantly lower plasma levels found humans treated with LE compared obtained those which alone administered. pharmacokinetic curves showed significant differences areas under plasma-time curve (AUC),...
Onivyde was approved by the Food and Drug Administration (FDA) in 2015 for treatment of solid tumors, including metastatic pancreatic cancer. It is designed to encapsulate irinotecan at high concentration, increase its blood-circulation lifetime, deliver it cells where enzymatically converted into SN-38, a metabolite with 100- 1000-fold higher anticancer activity. Despite rewarding clinical path, little known about physical state encapsulated within how this synthetic identity changes...
Human aquaporin 4 has been studied using non-equilibrium molecular dynamics simulations in the absence and presence of pulses external electric fields. The were 100 ns duration 0.005–0.015 V/Å intensity acting along pores’ axes. Water diffusivity dipolar response various residues interest within pores have studied. Results show relatively little change levels water permeability per se channels during axially oriented field impulses, although care must be taken with regard to statistical...
Electroporation characterization is a topic of intensive interest probed by extensive ongoing research efforts. Usually, these studies are carried out on lipid-bilayer electroporation. Surprisingly, the possibility water-channel electropore formation across transmembrane proteins themselves, particularly in view such promising application, has not yet been elucidated. The present work examines geometrical and kinetic aspects electropores their stability protein milieux (as opposed through...
Abstract Pro-inflammatory cytokines contribute to β-cell failure in both Type-1 and Type-2 Diabetes. Data collected so far allowed dissect the genomic, transcriptomic, proteomic biochemical landscape underlying cytokine-induced progression through dysfunction. Yet, no report thus complemented such molecular information with direct optical nanoscopy of subcellular environment. Here we tackle this issue Insulinoma 1E (INS-1E) β-cells by label-free fluorescence lifetime imaging microscopy...
This paper introduces an innovative approach utilizing Google Colaboratory for the versatile analysis of phasor fluorescence lifetime imaging microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in input file formats. In fact, phasor-FLIM widespread adoption has been hampered by complex instrumentation requirements. We mean to make advanced FLIM more accessible researchers through a cloud-based solution that 1) harnesses robust computational resources, 2)...
Electroporation is a matter of intensive ongoing research interest, and much-neglected topic in trans-membrane proteins, particularly view such promising potential applications medicine biotechnology.
Fluorescence lifetime analysis (FLA) has become essential for non-invasive, real-time of a variegate realm compounds and materials in scientific industrial fields. Here we present compact versatile device designed to apply FLA the screening healthcare nanoformulations which contain luminescent active principle. In case study here: Doxil enables decode supramolecular organization stability principle within nanoformulation rapid (seconds), cost-effective manner. Contrary currently used methods...
Abstract This study aims at creating an accessible notebook tool for the versatile analysis of phasor Fluorescence Lifetime Imaging Microscopy (FLIM) data collected from various samples (e.g. cuvette, cells, tissues) and in input file formats. The presented strategy facilitates morphological segmentations diverse mask imports. Results derived three compelling case studies involving cellular metabolism, nanoscale drug encapsulation (doxorubicin), impact pH metabolic cleavage on small...
Electroporation is subject to extensive research efforts and a rather under-studied in trans-membrane proteins, particularly considering promising possible applications biotechnology medicine. In particular, some such innovative potential are centred on controlling the permeability of ions through electro-pores. Here, we study ions’ conductivity along metastable electro-pores across h-AQP4 as typical case conduction membranes; note, achieving good degree control over this permeation presence...
Mohammad Reza Ghaani*, Mario Bernardi & Niall J. English*School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Ireland
<title>Abstract</title> It is widely accepted that the pH of insulin granules acidic, and its active regulation during granule maturation plays a role in process secretion by β-cells. Yet, calibrated measurement absolute with organelle specificity still lacking. To tackle this issue, we used genetically encoded E<sup>1</sup>GFP reporter inserted into C-peptide proinsulin expressed Insulinoma 1E cells. Following verification correct targeting granules, phasor-based Fluorescence Lifetime...
Treatment of both male and female rats with 5 IU/day mepartricin for 7–10 days administered by gastric tubing resulted in an increased faecal excretion some steroids. Mean rate elimination total oestrogens was enhanced 45% 14% rats, the average conjugated oestrogen also animals. Faecal cholesterol 37% 42% higher respectively, after treatment, plasma concentrations were reduced following treatment. It is suggested acts either changing intestinal flora or acting directly on steroid moieties,...