A5063624263- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- Gold and Silver Nanoparticles Synthesis and Applications
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- RNA regulation and disease
- Sphingolipid Metabolism and Signaling
- Lysosomal Storage Disorders Research
Scuola Normale Superiore
2022-2025
National Enterprise for NanoScience and NanoTechnology
2022-2023
International Flame Research Foundation
2023
Fondazione Pisa
2023
University of Pisa
2017-2021
University of Cambridge
2020-2021
Acid ceramidase (AC) is a lysosomal cysteine hydrolase that catalyzes the conversion of ceramide into fatty acid and sphingosine. This reaction lowers intracellular levels concomitantly generates sphingosine used for sphingosine-1-phosphate (S1P) production. Since increases in consequent decreases S1P reduce proliferation various cancers, AC might offer new target anti-tumor therapy. Here we CrispR-Cas9-mediated gene editing to delete encoding AC, ASAH1, human A375 melanoma cells. ASAH1-null...
It is widely accepted that the pH of insulin granules acidic, and its active regulation during granule maturation plays a role in process secretion by β-cells. Yet, calibrated measurement absolute with organelle specificity still lacking. To tackle this issue, we use genetically encoded E1GFP reporter inserted into C-peptide proinsulin expressed Insulinoma 1E cells. Following verification correct targeting granules, phasor-based Fluorescence Lifetime Imaging Microscopy (FLIM) applied to...
Lipid nanoparticles (LNPs) are currently having an increasing impact on nanomedicines as delivery agents, among others, of RNA molecules (e.g., short interfering for the treatment hereditary diseases or messenger development COVID-19 vaccines). Despite this, plasmid DNA (pDNA) by LNPs in preclinical studies is still unsatisfactory, mainly due to lack systematic structural and functional DNA-loaded LNPs. To tackle this issue, we developed, characterized, tested a library 16 multicomponent...
Gene editing may be used to excise the human immunodeficiency virus type 1 (HIV-1) provirus from host cell genome, possibly eradicating infection. Here, using cells acutely or latently infected by HIV-1 and treated with long terminal repeat (LTR)-targeting CRISPR/Cas9, we show that excised persists for a few weeks rearrange in circular molecules. Although proviral DNA is naturally formed during replication, observed gene might increase circles restored LTRs. These extrachromosomal elements...
PEGylated lipid nanoparticles (LNPs) are commonly used to deliver bioactive molecules, but the role of PEGylation in DNA-loaded LNP interactions at cellular and subcellular levels remains poorly understood. In this study, we investigated mechanism action LNPs using gene reporter technologies, dynamic light scattering (DLS), synchrotron small angle X-ray (SAXS), fluorescence confocal microscopy (FCS). We found that PEG has no significant impact on size or nanostructure DNA reduces their zeta...
<title>Abstract</title> It is widely accepted that the pH of insulin granules acidic, and its active regulation during granule maturation plays a role in process secretion by β-cells. Yet, calibrated measurement absolute with organelle specificity still lacking. To tackle this issue, we used genetically encoded E<sup>1</sup>GFP reporter inserted into C-peptide proinsulin expressed Insulinoma 1E cells. Following verification correct targeting granules, phasor-based Fluorescence Lifetime...
Early diagnosis is one of the most important factors in determining prognosis cancer. Sensitive detection and quantification tumour-specific biomarkers have potential to improve significantly our diagnostic capability. Here, we introduce a triggerable aptamer-based nanostructure based on an oligonucleotide/gold nanoparticle architecture that selectively disassembles presence biomarker interest; its optimization also in-silico determination aptamer nucleotides interactions with protein...
Abstract Gene editing may be used to cut out the human immunodeficiency virus type-1 (HIV-1) provirus from host cell genome and eradicate infection. Here, using cells acutely or latently infected by HIV treated with long terminal repeat-targeting CRISPR/Cas9, we show that excised persists for a few weeks and, means of Integrase, rearranges in circular molecules. Circularization integration restore proviral transcriptional activity is enhanced presence exogenous Tat Rev tumor necrosis...
Gene editing may be used to cut out the human immunodeficiency virus type-1 (HIV-1) provirus from host cell genome and eradicate infection. Here, using cells acutely or latently infected by HIV treated with long terminal repeat-targeting CRISPR/Cas9, we show that excised persists for a few weeks and, means of Integrase, rearranges in circular molecules. Circularization integration restore proviral transcriptional activity is enhanced presence exogenous Tat Rev tumor necrosis factor-α,...