A5082307968- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant Virus Research Studies
- Insect Resistance and Genetics
- HIV Research and Treatment
- Plant and animal studies
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Mosquito-borne diseases and control
- Transgenic Plants and Applications
- RNA regulation and disease
- Animal Disease Management and Epidemiology
- Insect Pest Control Strategies
- Photosynthetic Processes and Mechanisms
University of Cambridge
2018-2021
The Gurdon Institute
2019
Hebrew University of Jerusalem
2007-2014
We report the isolation, purification, genome-sequencing and characterization of a picorna-like virus from dead bees in Israel. Sequence analysis indicated that IAPV (Israeli acute paralysis virus) is distinct dicistrovirus. It most homologous to Kashmir bee virus. The carries 9487 nt RNA genome positive orientation, with two open reading frames separated by an intergenic region, its coat comprises four major proteins, sizes which suggest alternate processing polyprotein. virions also carry...
Abstract Colony Collapse Disorder (CCD) has been associated with Israeli acute paralysis virus (IAPV). CCD poses a serious threat to apiculture and agriculture as whole, due the consequent inability provide necessary amount of bees for pollination critical crops. Here we report on RNAi‐silencing IAPV infection by feeding double‐stranded RNA, an efficient feasible way controlling this viral disease. The association is discussed, well potential CCD.
The importance of honey bees to the world economy far surpasses their contribution in terms production; they are responsible for up 30% world's food production through pollination crops. Since fall 2006, U.S. have faced a serious population decline, due part phenomenon called Colony Collapse Disorder (CCD), which is disease syndrome that likely caused by several factors. Data from an initial study investigators compared pathogens affected CCD suggested putative role Israeli Acute Paralysis...
The mite Varroa destructor is an obligatory ectoparasite of the honey bee (Apis mellifera) and one major threats to apiculture worldwide. We previously reported that bees fed on double-stranded RNA (dsRNA) with a sequence homologous Israeli acute paralysis virus are protected from viral disease. Here we show dsRNA ingested by transferred parasitized bee. This cross-species, reciprocal exchange between engendered targeted gene silencing in latter, resulted over 60% decrease population. Thus,...
Systemic RNAi, initiated by double-stranded RNA (dsRNA) ingestion, has been reported in diverse invertebrates, including honey bees, demonstrating environmental uptake that undermines homologous gene expression. However, the question why any organism would take up from environment remained largely unanswered. Here, we report on horizontal flow among bees mediated secretion and ingestion of worker royal jelly diets. We demonstrate transmission jelly-secreted dsRNA to larvae is biologically...
RNA flow between organisms has been documented within and among different kingdoms of life. Recently, we demonstrated horizontal transfer honeybees involving secretion ingestion worker royal jellies. However, how the jelly facilitates is still unknown. Here, show that jellies harbor robust RNA-binding activity. We report a highly abundant component, major protein 3 (MRJP-3), acts as an extracellular non-sequence-specific RNA-aggregating factor. Multivalent binding stimulates higher-order...
Mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses other parasitic microorganisms. The emergence spread insecticide resistance is threatening the effectiveness current control measures for common mosquito vector borne diseases, malaria, dengue Zika. Therefore, emerging widely used pyrethroid insecticides an alarming problem public health. Herein we demonstrated use RNA interference (RNAi) increase susceptibility adult mosquitoes...
Abstract Multigene expression is required for metabolic engineering, i.e. coregulated of all genes in a pathway the production desired secondary metabolite. To that end, several transgenic approaches have been attempted with limited success. Better success has achieved by transforming plastids operons. IL-60 platform constructs driven from geminivirus Tomato yellow leaf curl virus. We demonstrate enables nontransgenic an entire bacterial operon tomato (Solanum lycopersicum) plants without...
Gene editing may be used to excise the human immunodeficiency virus type 1 (HIV-1) provirus from host cell genome, possibly eradicating infection. Here, using cells acutely or latently infected by HIV-1 and treated with long terminal repeat (LTR)-targeting CRISPR/Cas9, we show that excised persists for a few weeks rearrange in circular molecules. Although proviral DNA is naturally formed during replication, observed gene might increase circles restored LTRs. These extrachromosomal elements...
The IL-60 platform, consisting of a disarmed form tomato yellow leaf curl virus (TYLCV) and auxiliary components, was previously developed as nontransgenic universal vector system for gene expression silencing that can express an entire operon in plants. does not allow rolling-circle replication; hence, production viral single-stranded (ss) DNA progeny is prevented. We used this double-stranded (ds) DNA-restricted platform (uncoupled from the dsDNA→ssDNA replication phase DNA) functional...
ABSTRACT One of the characteristics RNA interference (RNAi) is systemic spread silencing signal among cells and tissues throughout organism. Systemic RNAi, initiated by double-stranded (dsRNA) ingestion, has been reported in diverse invertebrates, including honey bees, demonstrating environmental uptake that undermines homologous gene expression. However, question why any organism would take up from environment remained largely unanswered. Here, we report on horizontal flow bees mediated...
Abstract Gene editing may be used to cut out the human immunodeficiency virus type-1 (HIV-1) provirus from host cell genome and eradicate infection. Here, using cells acutely or latently infected by HIV treated with long terminal repeat-targeting CRISPR/Cas9, we show that excised persists for a few weeks and, means of Integrase, rearranges in circular molecules. Circularization integration restore proviral transcriptional activity is enhanced presence exogenous Tat Rev tumor necrosis...
Gene editing may be used to cut out the human immunodeficiency virus type-1 (HIV-1) provirus from host cell genome and eradicate infection. Here, using cells acutely or latently infected by HIV treated with long terminal repeat-targeting CRISPR/Cas9, we show that excised persists for a few weeks and, means of Integrase, rearranges in circular molecules. Circularization integration restore proviral transcriptional activity is enhanced presence exogenous Tat Rev tumor necrosis factor-α,...