A5051147546- Nanoparticle-Based Drug Delivery
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Protein Hydrolysis and Bioactive Peptides
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Cannabis and Cannabinoid Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Analytical chemistry methods development
- GABA and Rice Research
- Meat and Animal Product Quality
- Mycotoxins in Agriculture and Food
- Biochemical Analysis and Sensing Techniques
- Characterization and Applications of Magnetic Nanoparticles
- Polymer Surface Interaction Studies
- Pesticide Residue Analysis and Safety
- Identification and Quantification in Food
- Phytochemicals and Antioxidant Activities
- Effects and risks of endocrine disrupting chemicals
- Biosensors and Analytical Detection
- Protein Interaction Studies and Fluorescence Analysis
- Diet, Metabolism, and Disease
- Pharmaceutical and Antibiotic Environmental Impacts
- Lipid Membrane Structure and Behavior
Sapienza University of Rome
2016-2025
European University of Rome
2022
University of Naples Federico II
2019-2020
University of Campania "Luigi Vanvitelli"
2019-2020
Naples Anesthesia & Physician Associates
2019-2020
Multidisciplinary Digital Publishing Institute (Switzerland)
2019
Institute for the Chemistry of OrganoMetallic Compounds
2019
When nanoparticles (NPs) enter a physiological environment, medium components compete for binding to the NP surface leading formation of rich protein shell known as "protein corona". Unfortunately, opsonins are also adsorbed. These proteins immediately recognized by phagocyte system with rapid clearance NPs from bloodstream. Polyethyleneglycol (PEG) coating (PEGylation) is most efficient anti-opsonization strategy. Linear chains PEG, grafted onto surface, able create steric hindrance,...
When nanoparticles (NPs) enter a biological fluid (e.g., human plasma (HP)), proteins and other biomolecules adsorb on the surface leading to formation of rich protein shell, referred as "protein corona". This corona is dynamic in nature its composition varies over time due continuous association dissociation events. Understanding evolution time-scales particle's lifetime blood fundamental predict fate vivo. In this study, we used lipid NPs, cationic...
Abstract In vivo liposomes, like other types of nanoparticles, acquire a totally new ‘biological identity’ due to the formation biomolecular coating known as protein corona that depends on and modifies liposomes’ synthetic identity. The liposome–protein is dynamic interface regulates interaction liposomes with physiological environment. Here we show biological identity clearly linked their sequestration from peripheral blood mononuclear cells (PBMCs) healthy donors ultimately leads removal...
(-)-Trans-Δ9-tetrahydrocannabinol (Δ9-THC) is the main compound responsible for intoxicant activity of Cannabis sativa L. The length side alkyl chain influences biological this cannabinoid. In particular, synthetic analogues Δ9-THC with a longer have shown cannabimimetic properties far higher than itself. attempt to define phytocannabinoids profile that characterizes medicinal cannabis variety, new phytocannabinoid same structure but seven-term was identified. natural isolated and fully...
A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers vivo, can be used for targeting. Here, we show that nanoparticles acquire a selective targeting capability with corona adsorbed on surface. We demonstrate lipid particles made of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and DNA, upon interaction human plasma components, spontaneously become coated vitronectin promotes efficient uptake cancer cells expressing high levels...
The structure and composition of the liposome–protein corona are dependent on incubation protocol (<italic>i.e.</italic> dynamic <italic>vs.</italic> static incubation).
When injected in a biological milieu, nanomaterial rapidly adsorbs biomolecules forming biomolecular corona. The corona changes the interfacial composition of giving it identity that determines physiological response. Characterization structure and has received increasing attention mostly due to its detrimental impact on nanomaterial's metabolism vivo. It is generally accepted an opsonin-enriched promotes immune system recognition rapid clearance from circulation. Here we applied dynamic...
As soon as nanomaterials, such nanoparticles (NPs), are injected into a physiological environment rich coating of biomolecules known the "protein corona" rapidly covers them. This protein dress is main factor, which affects interaction NPs with living systems. While relationship between NP features and biomolecule corona has been extensively investigated, whether how changes in affect NP–protein remains under-investigated. one most important steps translating results animal models to clinic....
Protein corona sensor array technology identifies diseases through specific proteomics pattern recognition.
For several decades, surface grafted polyethylene glycol (PEG) has been a go-to strategy for preserving the synthetic identity of liposomes in physiological milieu and preventing clearance by immune cells. However, limited clinical translation PEGylated is mainly due to protein corona formation subsequent modification liposomes' identity, which affects their interactions with cells blood residency. Here we exploit electric charge DNA generate unPEGylated liposome/DNA complexes that, upon...
Abstract Cannabis is a multifaceted plant with numerous therapeutic properties on one hand, and controversial psychotropic activities the other which are modulated by CB1 endocannabinoid receptors. Δ9-Tetrahydrocannabinol (Δ9-THC) has been identified as main component responsible for effects, while its constitutional isomer cannabidiol (CBD) shown completely different pharmacological properties. Due to reported beneficial gained global popularity openly sold in shops online. To circumvent...
The concept that the effective unit of interest in cell-nanomaterial interaction is particle and its corona associated proteins emerging. Here we investigate compositional evolution protein 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) DOTAP/DNA lipoplexes over a wide range plasma concentrations (2.5-80%). composition hard quite stable, but CLs does evolve considerably. We show made both low-affinity competitive-binding whose relative abundance changes with...