A5088483888- Meat and Animal Product Quality
- Mycotoxins in Agriculture and Food
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Biochemical Analysis and Sensing Techniques
- Protein Hydrolysis and Bioactive Peptides
- Antioxidant Activity and Oxidative Stress
- Estrogen and related hormone effects
- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Plant biochemistry and biosynthesis
- Phytoestrogen effects and research
- Phytase and its Applications
- Proteins in Food Systems
- Plant and fungal interactions
- Indoor Air Quality and Microbial Exposure
- Mathematics Education and Teaching Techniques
- Aquaculture disease management and microbiota
- Pesticide Residue Analysis and Safety
- Cognitive and developmental aspects of mathematical skills
- Bone health and treatments
- Plant Pathogens and Fungal Diseases
- Seed and Plant Biochemistry
Sapienza University of Rome
2012-2016
This paper describes a novel and efficient analytical method to define the profile of fat-soluble micronutrients in milk from different animal species. Overnight cold saponification was optimized as simultaneous extraction procedure. Analytes were separated by nonaqueous reversed-phase (NARP) chromatography: carotenoids on C30 column vitamins tandem C18 system. Besides 12 target analytes for which standards are available (all-trans-lutein, all-trans-zeaxanthin, all-trans-β-cryptoxanthin,...
A novel method for the simultaneous determination of enniatins A, A1, B and B1 beauvericin, both in human urine plasma samples, was developed validated. The consisted a simple easy pretreatment, specific each matrix, followed by solid phase extraction (SPE) detection high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. optimized SPE performed on graphitized carbon black cartridges after suitable dilution extracts, which allowed mycotoxin absolute...
A simple, fast, and reproducible method for the simultaneous determination of natural estrogens mycoestrogens (resorcylic acid lactones) in milk by ultrahigh-performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (UHPLC/ESI-MS/MS) is described. The extraction was carried out solid-phase (SPE) using graphitized carbon black as solid sorbent. use allowed us to avoid any type sample pretreatment, performed simply diluting samples...
A shotgun proteomics approach was used to characterize the quinoa seed proteome. To obtain comprehensive proteomic data from seeds three different precipitation procedures were employed: MeOH/CHCl 3 /double‐distilled H 2 O, acetone either alone or with trichloroacetic acid; isolated proteins then in‐solution digested and resulting peptides analyzed by nano‐liquid chromatography coupled tandem mass spectrometry. However, since is a nonmodel plant species, only few protein sequences are...
This paper presents an analytical strategy for a large-scale screening of carotenoids in tomato fruits by exploiting the potentialities triple quadrupole-linear ion trap hybrid mass spectrometer (QqQLIT). The method involves separation on C30 reversed-phase column and identification means diode array detection (DAD) atmospheric pressure chemical ionization-mass spectrometry (APCI-MS). authentic standards six model compounds were used to optimize separative conditions predict chromatographic...
Phenolic acids are secondary metabolites of plants. Although less studied other phenolic compounds such as flavonoids, the interest in their determination is also due to antioxidant activity. In past, gas chromatography coupled with mass spectrometry detection (GC–MS), well liquid (LC) UV detection, have been widely used for determining acids. Recently, significant improvements LC technology (for example, introduction ultra-high performance systems) MS instrumentation (with development last...
UV filters, contained in sunscreens and other cosmetic products, as well some plastics industrial are nowadays considered contaminants of emerging concern because their widespread increasing use has lead to presence the environment. Furthermore, filters suspected have endocrine disruption activity. In present work, we developed an analytical method based on liquid chromatography with tandem mass spectrometry for determination tap lake waters. Sixteen were extracted from water samples by...
Natural estrogens are synthesized by mammals in different amounts depending on the developmental stage and pregnancy/lactation period, they may pass into milk, where mostly present as glucuronated sulfated forms. In modern dairy practices, about 75% of milk is produced from pregnant cows; therefore, amount hormones that could be concern. While estrogen determination has been investigated depth, individual their conjugated forms products not fully addressed. The aim this work was to develop...
Recently, magnetic solid-phase extraction has gained interest because it presents various operational advantages over classical extraction. Furthermore, nanoparticles are easy to prepare, and materials can be used in their synthesis. In the literature, there only few studies on determination of mycoestrogens milk, although carryover milk occurred. this work, we wanted develop first (to best our knowledge) protocol for six from followed by liquid chromatography tandem mass spectrometry...
This paper describes an analytical approach, based on LC-diode array detector-MS/MS (LC-DAD-MS/MS), for characterizing the fat-soluble micronutrient fraction in rainbow trout (Oncorhynchus mykiss). Two different procedures were applied to isolate analytes from liver and muscle tissue: overnight cold saponification hydrolyze bound forms simplify analysis; matrix solid-phase dispersion avoid artifacts maintain unaltered naturally occurring forms. Analytes separated a C30 column by using...
Rationale Sulfur‐vulcanized rubber is a three‐dimensional polymer network, insoluble in all organic solvents. For this reason, vulcanization products are difficult to study and identify by conventional analytical techniques. To simplify task, low molecular weight olefins have been used as model compounds (MCs) place of experiments. Methods In work, the process was investigated using squalene (SQ) MC. By‐products, intermediates were separated semipreparative reversed‐phase liquid...