A5103197871- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Nanocluster Synthesis and Applications
- Immunotherapy and Immune Responses
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Molecular Junctions and Nanostructures
- Chemical Synthesis and Analysis
- Force Microscopy Techniques and Applications
- Metabolism and Genetic Disorders
- Listeria monocytogenes in Food Safety
- Mitochondrial Function and Pathology
- MRI in cancer diagnosis
- Magnetic properties of thin films
- Analytical Chemistry and Chromatography
- Asymmetric Synthesis and Catalysis
- Neurogenesis and neuroplasticity mechanisms
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2008-2017
CIC biomaGUNE
2008-2017
Centro de Investigación Biomédica en Red
2012-2017
Research Network (United States)
2017
Ayuntamiento de San Sebastián
2017
Bioengineering Center
2014
CIC bioGUNE
2012
Ikerbasque
2012
Consejo Superior de Investigaciones Científicas
1986-2009
Universidad de Zaragoza
2009
Abstract A simple and versatile methodology is described for tailoring sugar‐functionalised gold nanoclusters ( glyconanoparticles ) that have 3D polyvalent carbohydrate display globular shapes. This allows the preparation of with biologically significant oligosaccharides as well differing density. Fluorescent been also prepared labelling cells in biological tests. The materials are water soluble, stable under physiological conditions present an exceptional small core size. All them...
Coupling of capsular polysaccharides pathogens to immunogenic protein carriers (conjugate vaccines) improves carbohydrate immune response. Our idea is explore gold nanoclusters as prepare fully synthetic vaccines.Gold glyconanoparticles bearing a tetrasaccharide epitope related the Streptococcus pneumoniae type 14 polysaccharide (Pn14PS), T-helper ovalbumin 323-339 peptide (OVA(323-339)), and D-glucose were prepared by one-pot method. Their immunogenicity was tested in mice. Cytokine levels...
Poly(ethylene glycol) (PEG) has become the gold standard for stabilization of plasmonic nanoparticles (NPs) in biofluids, because it prevents aggregation while minimizing unspecific interactions with proteins. Application Au NPs biological environments requires use ligands that can target selected receptors, even presence protein-rich media. We demonstrate here stabilizing effect low-molecular-weight glycans on both spherical and rod-like under physiological conditions, as bench-marked...
New tools and techniques to improve brain visualization assess drug permeability across the blood-brain barrier (BBB) are critically needed. Positron emission tomography (PET) is a highly sensitive, noninvasive technique that allows evaluation of BBB under normal disease-state conditions. In this work, we have developed synthesis novel water-soluble biocompatible glucose-coated gold nanoparticles (GNPs) carrying BBB-permeable neuropeptides chelator positron emitter (68)Ga as PET reporter for...
More evidence for carbohydrate self-interactions as a first step in cell adhesion and recognition has been found experiments with gold glyconanoparticles self-assembled monolayers presenting epitopes (see picture). The Ca2+-ion-dependent interactions were studied quantified by surface plasmon resonance. Gray circles=gold nanoparticles, black circles=Ca2+ ions.
Dendritic cells are the most potent of professional antigen-presenting which display a pivotal role in generation and regulation adaptive immune responses against HIV-1. The migratory nature dendritic is subverted by HIV-1 to gain access lymph nodes where viral replication occurs. express several calcium-dependent C-type lectin receptors including cell-specific ICAM-3 grabbing non-integrin (DC-SIGN), constitute major receptor for DC-SIGN recognizes N-linked high-mannose glycan clusters on...
The development of sensors to detect specific weak biological interactions is still today a challenging topic. Characteristics carbohydrate-protein (lectin) include high specificity and low affinity. This work describes the nanostructured impedimetric for detection concanavalin A (Con A) binding immobilized thiolated carbohydrate derivatives (D-mannose or D-glucose) onto screen-printed carbon electrodes (SPCEs) modified with gold nanoparticles. Thiolated D-galactose derivative was employed...
In the search for an effective vaccine against human pathogen, Listeria monocytogenes (Listeria), gold glyconanoparticles (GNP) loaded with a listeriolysin O peptide LLO91–99 (GNP-LLO) were used to immunise mice, initially using dendritic cell (DC) approach, but subsequently standard parenteral immunisation approach. To enhance immunogenicity novel polysaccharide adjuvant based on delta inulin (Advax™) was also co-formulated GNP vaccine. Confirming previous results, DC in vitro GNP-LLO...
The therapeutic approach for the treatment of HIV infection is based on highly active antiretroviral therapy (HAART), a cocktail drugs. Notwithstanding HAART has shown different drawbacks like toxic side effects and emergence viral multidrug resistance. Nanotechnology offers new tools to improve drug prevention. In this scenario, gold nanoparticles are an interesting chemical tool design prepare smart efficient drug-delivery systems. Here we describe preparation antiviral activity...
Aim: Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two antigens related serotypes 19F and 14 of Streptococcus pneumoniae evaluated their immunogenicity in vivo. Results: A tetrasaccharide fragment serotype (Tetra-14), a trisaccharide (Tri-19F), T-helper peptide d -glucose were loaded onto GNP different ratios. Mice immunization showed that the...
Abstract CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that expression increased at inflammatory sites, pointing the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging expressing macrophages an interesting strategy order to detect atherosclerotic We prepared targeted probe based on gold-coated iron oxide nanoparticles vectorized with anti-CD163 antibody for specific detection MRI. Firstly,...
The adhesion forces between individual molecules of Lewis(X) trisaccharide antigen (Le(X) ) have been measured in water and calcium solution by using atomic force microscopy (AFM, see graph). These results demonstrate the self-recognition capability this antigen, reinforce hypothesis that carbohydrate-carbohydrate interaction could be considered as first step cell-adhesion process nature.
Combining sugar conjugates and DO3A-Gd complexes, paramagnetic gold glyconanoparticles (GNPs) with different relaxivity values were obtained tested in vivo as MRI probes.
Galactofuranose (Galf) is the five-membered ring form of galactose exclusively found in nonmammalian species, among which several are pathogens. To determine putative role this carbohydrate host–pathogen interactions, we synthesized multivalent gold nanoparticles carrying Galf (Galf-GNPs) and show that they recognized by EB-A2 antibody, widely used to detect Galf-containing galactomannan serum Aspergillosis patients. We demonstrated human monocyte-derived dendritic cells bound Galf-GNPs via...
Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present targeting device developed using gold nanoparticles functionalized α-fucosyl-β-alanyl amide. The bound to cellular induced internalization as effectively similar particles...