A5060651752- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Pneumonia and Respiratory Infections
- Boron Compounds in Chemistry
- Tryptophan and brain disorders
- Organoboron and organosilicon chemistry
- Radiopharmaceutical Chemistry and Applications
- Complement system in diseases
- T-cell and B-cell Immunology
- Pharmacological Receptor Mechanisms and Effects
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Stress Responses and Cortisol
- Bacterial Infections and Vaccines
- RNA Interference and Gene Delivery
- Gut microbiota and health
- Cardiac Fibrosis and Remodeling
- Boron and Carbon Nanomaterials Research
- Infrared Thermography in Medicine
- Mast cells and histamine
- Bipolar Disorder and Treatment
- Pharmacological Effects and Toxicity Studies
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2015-2024
Istituto di Farmacologia Traslazionale
2014-2015
University of Pavia
2005-2007
Istituti di Ricovero e Cura a Carattere Scientifico
2005
Phenolic compounds exert cytoprotective effects; our purpose was to investigate whether the isosteric polyphenolic clovamide and rosmarinic acid are neuroprotective.
Aim: Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two antigens related serotypes 19F and 14 of Streptococcus pneumoniae evaluated their immunogenicity in vivo. Results: A tetrasaccharide fragment serotype (Tetra-14), a trisaccharide (Tri-19F), T-helper peptide d -glucose were loaded onto GNP different ratios. Mice immunization showed that the...
In this study, a successful medicinal chemistry campaign that exploited virtual, biophysical, and biological investigations led to the identification of novel class IDO1 inhibitors based on benzimidazole substructure. This family compounds is endowed with an extensive bonding network in protein active site, including interaction pocket C, region not commonly by previously reported inhibitors. The tight packing selected within enzyme contributes strong binding IDO1, inhibitory potency at low...
The adaptive changes that develop in the pressure-overloaded left ventricular (LV) myocardium include cardiomyocyte hypertrophy and interstitial fibrosis. Although former is known to depend a sizeable extent on sympathetic (over)activity, little information exists whether same applies latter, ie, excess catecholamine exposure contributes imbalance between collagen deposition by fibroblasts degradation matrix metalloproteases (MMPs), eventually leading LV accumulation. Sprague-Dawley rats...
We have synthesized and characterized nearly monodisperse highly pure gold nanoparticles (2 5 nm) coated with non-immunoactive mono- disaccharides, modelled after the capsular polysaccharide of serogroup A Neisseria meningitidis bacterium. used them to test their ability induce immune cell responses as a consequence multivalency. The results indicate that they are indeed immunoactive immunoactivity is strongly dependent on size, larger, nm perform far better than smaller, 2 ones. Immune...
Antigen analogs of polyclonal antibodies specific for type A Neisseria mengitidis are prepared by covering gold clusters (2–5 nm in size) with a monolayer saccharides that have the structure repeating unit capsular polysaccharide this bacterium. These systems may find use as synthetic antigens immunostimulation.
In osteosarcoma (OS) patients, only a limited number of drugs are active and the regimens currently in use include combination at least two these drugs: doxorubicin, cisplatin, methotrexate ifosfamide. Today, 30-40% patients still die OS highlighting urgent need for new treatments. Invariant NKT (iNKT) cells lymphocyte lineage with features both T NK cells, playing important roles tumour suppression. Our aim was to test whether cytoxicity induced by doxorubicin against can be enhanced iNKT...
A virtual library of 50 000 compounds synthesizable <italic>via</italic> the van Leusen MCR was created, screened and filtered to afford a series disubstituted imidazoles with improved properties compared 4-phenylimidazole.
Some new phosphonoester-linked oligomers, stabilized analogues of the corresponding phosphate-bridged oligomers Neisseria meningitidis A (MenA) capsular polysaccharide (CPS), were conjugated to human serum albumin (HSA), as a protein carrier model, and studied for immunological activities. We determined (i) in vitro, their biocompatibility (CAM test) activity inducing both T cell proliferation (CFSE method) IL-2 release (ELISA), (ii) vivo, ability stimulate specific IgG antibody production...
IDO1, a key dioxygenase in tryptophan-kynurenine metabolism, appeared the last 10 years at vanguard of druggable targets cancer therapy due to its well-established role both immune escape and inflammatory neovascularization. Among pool IDO1 inhibitors that have entered clinical trials, none reached approval. The identification novel endowed with better profile, together further comprehension interactions residues active site, are still need. In this context, we synthesized class...
Dodecaborate anions represent a suitable boron source for neutron capture therapy (BNCT). Sugar derivatives of dodecaborate have been prepared through the ring‐opening reaction cyclic oxonium ions by alkoxide nucleophiles. According to this general approach, convenient and short synthesis three dodecaborate‐sugar conjugates is herein reported. Glucose, galactose fructose chosen their expected preferential uptake tumor cells compared healthy cells. The free hydroxyl group isopropylidene...
A new practical synthesis of α-GalCer and its analogues is presented, opening the chance to easily modify sphingosine chain. The common precursor a disaccharide, obtained by coupling tetra-O-benzyl-d-galactose with allyl 2,3-O-isopropylidene-d-lyxofuranoside. Introduction alkyl chains via Wittig reaction (for OCH) or Williamson oxa analogues) followed standard synthetic steps allows one efficiently obtain such compounds. are able activate iNKT cells when presented CD1d expressing cells.
During the transition of pressure overload hypertrophy (POH) to heart failure (HF) there is intense interstitial cardiac remodeling, characterized by a complex balance between collagen deposition and degradation matrix metalloproteases (MMPs). This study was aimed at investigating process remodeling during different phases POH HF.Guinea pigs underwent thoracic descending aortic banding or sham operation. Twelve weeks after surgery, left-ventricular (LV) end-diastolic internal dimension...
The synthesis of a carba-analogue corresponding to the trisaccharide repeating unit Streptococcus pneumoniae type 19F capsular polysaccharide, where residue carba-L-rhamnose has been inserted into natural in place L-rhamnose, is described. conformational properties analogue were investigated with aid molecular dynamics simulations and strictly analogous those compound. biological activity was comparable that unit, thus suggesting this compound, more stable hydrolysis, good mimic structure.
Among new therapies to fight tumors, immunotherapy is still one of the most promising and intriguing. Thanks ongoing structural elucidation several tumor antigens development innovative antigen carriers, in constant evolution it largely used either alone or synergy with chemotherapy/radiotherapy. With aim develop fully synthetic immunostimulants we have recently developed a mimetic α-Tn mucin antigen, relevant antigen. The (4)C1 blocked 1, unique example an was functionalized ω-phosphonate...
To date, sugar analogues that contain boronic acids as substitutes for hydroxyl groups are a class of compounds nearly unknown in the literature. The challenging synthesis two sugar–boronic acid is described, and data retrieved on their solution behavior, stability, toxicity. As these were expected to mimic behavior carbohydrates, they tested regards future development potential boron neutron capture therapy agents.
Specific drugs and/or immunotherapies are widely used to treat allergies, but drug-induced adverse effects recently led explore new additional strategies. We studied whether a probiotic preparation (iPROB®; Anallergo SpA, Florence, Italy) is effective in allergic patients and the mechanisms underlying clinical outcomes.Eligible (n = 28), all suffering from rhinitis with/without bronchial asthma, were consecutively recruited at Allergology Medical Unit (Novara, treated with this probiotic....
After the development of accelerators as neutron source, access to new suitable agents for boron capture therapy (BNCT) became a major need. Among many others, sugar boronic acids have recently attracted attention carriers. Herein we report synthesis and preliminary biological studies two analogues containing acid at anomeric position. The were obtained by hydroboration proper open-chain terminal alkenes that, after quenching with water, spontaneously afforded cyclic hemiacetal-like structures.